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Rpmi 1640

Manufactured by Keygene
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Sourced in China

RPMI-1640 is a media formulation commonly used for the in vitro culture of various cell types, including lymphocytes and other mammalian cells. It provides a balanced salt solution with amino acids, vitamins, and other nutrients essential for cell growth and maintenance.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Keygene (2 mentions) H1975, by Keygene (1 mentions) H1299, by Keygene (1 mentions) Nci h1299, by Keygene (1 mentions)

Close spelling variants of this product

RPMI 1640 medium (10 citations)

2 protocols using rpmi 1640

1

Glucose Starvation in Human Lung Cells

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Human bronchial epithelial cell (HBE) and lung adenocarcinoma cell lines (A549, H1299, PC9 and H1975) were purchased from Shanghai Institutes for Biological Science (Shanghai, China). HBE and H1975 were cultivated in DMEM (KeyGene, Nanjing, China), while A549, H1299 and PC9 cells were cultivated in RPMI-1640(KeyGene, Nanjing, China), all supplemented with 10% FBS (KeyGene, Nanjing, China) and cultured in a standard 37℃ incubator. For glucose starvation experiments, cells were cultured in medium with different concentrations of glucose.
Luo J., Wang H., Wang L., Wang G., Yao Y., Xie K., Li X., Xu L., Shen Y., & Ren B. (2020). Long Noncoding RNA GAS6-AS1 Inhibits Progression and Glucose Metabolism Reprogramming in Lung Adenocarcinoma Via Repressing E2F1-Mediated Transcription of GLUT1.
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2

Cell Culture Conditions for Cancer Research

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All cell lines [PC9, A549, NCI-1975, NCI-H1299, and human bronchial epithelial cell (HBE)] were purchased from the Shanghai Institute for Biological Science, China. PC9, A549, NCI-1975, and NCI-H1299 cells were cultivated in DMEM (KeyGene, Nanjing, China) with high glucose (4.5 g/L) and 10% fetal bovine serum, and HBE cells were cultivated in RPMI 1640 (KeyGene, Nanjing, China), supplemented with 10% fetal bovine serum. All cell lines were maintained in a humidified incubator at 37 °C (5% CO2).
Xie K., Feng J., Fan D., Wang S., Luo J., Ren Z., Zheng C., Diao Y., De Mello R.A., Tavolari S., Brandi G., Roden A.C., Ren B., Shen Y, & Xu L. (2022). BARX2/FOXA1/HK2 axis promotes lung adenocarcinoma progression and energy metabolism reprogramming. Translational Lung Cancer Research, 11(7), 1405-1419.
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