Copper (II) Acetate monohydrate (CuAc, 98%) and Cobalt (II) acetate tetra hydrate (CoAc, 98%) were bought from Showa chemicals co Ltd Japan and Junsei chemicals co Ltd Japan, respectively. Poly (Vinyl alcohol) PVA with a molecular weight 65000 g/mol was obtained from Aldrich, USA, Distilled water was used as solvent.
Poly vinyl alcohol pva
Manufactured by Merck Group
Sourced in United States, United Kingdom
Poly(vinyl alcohol) (PVA) is a synthetic, water-soluble polymer. It is a versatile material commonly used in various laboratory applications due to its unique properties, such as high tensile strength, flexibility, and chemical resistance.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Maleic anhydride, by Merck Group (1 mentions)
Vitamin b12, by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
N n dimethylacrylamide, by Merck Group (1 mentions)
Sulfuric acid, by Carlo Erba (1 mentions)
Acetic acid, by Carlo Erba (1 mentions)
Glycerol, by Merck Group (1 mentions)
Sodium tetraborate, by Merck Group (1 mentions)
Polydiallyldimethylammonium chloride pdadmac, by Merck Group (1 mentions)
Sodium borohydride, by Thermo Fisher Scientific (1 mentions)
Citric acid, by Thermo Fisher Scientific (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Polyethylene glycol peg, by Thermo Fisher Scientific (1 mentions)
Polycaprolactone, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Potassium phosphate dibasic, by Merck Group (1 mentions)
Hepes, by Lonza (1 mentions)
9 protocols using poly vinyl alcohol pva
1
Copper-Cobalt Acetate Composite Synthesis
2
Helical Coronary Artery Scaffold Design
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Materials: Poly (vinyl alcohol) (PVA, 99% hydrolyzed, number average MW 89,000-98,000 g/mol) were obtained from Aldrich Chemical Co (UK). Gelatin (Ge) Type A (Approx. 300
Bloom) from porcine skin were obtained in powder form (Aldrich Chemical Co., UK).
Methanol (99% Pure, HPLC grade), glacial acetic acid (99% pure) were obtained from Fisher scientific; Human Coronary Artery Endothelial Cells (HCAEC), passage 4 (PromoCell, Germany). Physiological Salt Solution (PSS) was prepared as previously described. 33 (link) Scaffold design and fabrication: Using computational fluid dynamic (CFD) simulation, a new scaffold structure with a small diameter of 4mm was designed with inner helices to enable a
Bloom) from porcine skin were obtained in powder form (Aldrich Chemical Co., UK).
Methanol (99% Pure, HPLC grade), glacial acetic acid (99% pure) were obtained from Fisher scientific; Human Coronary Artery Endothelial Cells (HCAEC), passage 4 (PromoCell, Germany). Physiological Salt Solution (PSS) was prepared as previously described. 33 (link) Scaffold design and fabrication: Using computational fluid dynamic (CFD) simulation, a new scaffold structure with a small diameter of 4mm was designed with inner helices to enable a
3
Protein-Functionalized Polymer Synthesis
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Bovine serum albumin, BSA (M w 68,000 g mol -1 , ≥98.0%), maleic anhydride, MAy (≥99.0%), ammonium persulfate, APS, (≥98.0%) and vitamin B 12 (≥98.5%) were purchased from Sigma-Aldrich. N ' ,N ' -dimethylacrylamide, DMAAm (99 %) and poly(vinyl alcohol) -PVA (87-89% hydrolyzed, M w 31.000 g mol -1 ) were obtained from Aldrich. Benzyl alcohol (99.0%) and absolute ethyl alcohol (≥99.3%) were supplied by Fmaia, whilst N,N,N',N'-tetramethylethylenediamine, TEMED (d = 0.780 g.mL -1 , ≥99.0%) was obtained from Invitrogen. For buffer solutions, sodium phosphate, dibasic, anhydrous (98.0%) were supplied by Nuclear, and potassium phosphate, monobasic, (99.0%) from Labsynth. Buffer solutions of pH 7 and 10 were obtained from Synth. The hydrochloric acid (36.5-38.0%) and sodium chloride (Synth, 99.0%) were supplied by Chemco and Synth, respectively. All chemical were used as received. Protein-functionalizing solution was prepared by adding 0.5 g of BSA and 0.05 g of MAy to a phosphate buffer solution (0.1 M) with pH 5.5 while stirring. After homogenization, the solution was stirred for 3 h at 37 ºC. The obtained product was precipitated with cold ethanol (2 °C), subsequently separated by centrifugation at 9500 rpm, and washed with deionized-distilled water three times.
4
Bismuth Oxide Nanopowder Textile Fabrication
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Bismuth(III) oxide (Bi2O3) nanopowder with the particle size of 90–210 nm and 99.9%
purity and Bi2O3 microparticles with the particle
size of 10 μm and 99.9% purity were purchased from Aldrich.
Poly(vinyl alcohol) (PVA), Mw 89 000–98 000
g/mol with 99% hydrolyzed, was also purchased from Aldrich. Glutaraldehyde
(1.2% w/v) was obtained from Fluka. Sulfuric acid, acetic acid, and
methanol were purchased from Carlo Erba Reagenti SpA, J. T. Baker,
and Fisher Chemicals, respectively. Polyester and cotton fabrics were
purchased from a local market in Chonburi Province, Thailand.
purity and Bi2O3 microparticles with the particle
size of 10 μm and 99.9% purity were purchased from Aldrich.
Poly(vinyl alcohol) (PVA), Mw 89 000–98 000
g/mol with 99% hydrolyzed, was also purchased from Aldrich. Glutaraldehyde
(1.2% w/v) was obtained from Fluka. Sulfuric acid, acetic acid, and
methanol were purchased from Carlo Erba Reagenti SpA, J. T. Baker,
and Fisher Chemicals, respectively. Polyester and cotton fabrics were
purchased from a local market in Chonburi Province, Thailand.
5
Hydrogel Formulation with PVA, Dextrose, Glycerol
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Poly(vinyl alcohol) (PVA), dextrose (≥99.5%), glycerol (≥99.0%) and sodium tetraborate (99.98%) were used as received from Sigma Aldrich. Scheme 2. Schematic representation for the hydrogel preparation.
6
Synthesis and Characterization of Modified PVA Polymers
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Poly(vinyl alcohol) (PVA, average Mw = 85,000–124,000 g·mol−1, degree of hydrolysis = 99%) was purchased from Aldrich (Milwaukee, WI, USA), poly (diallyl dimethyl ammonium chloride) (PDADMAC) was purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO, USA) and poly(vinyl alcohol-co-2-acrylamido-2-methyl propane sulfonic acid) (AP-2, average Mw = 70,000 g·mol−1, degree of hydrolysis = 99.5%) was obtained from KURARAY Co., Ltd. (Tokyo, Japan). AP-2 is a modified PVA that contains 2 mol% of 2-acrylamido-2-methylpropane sulfonic acid groups as a copolymer, and, hence, provides cation-exchange sites. Commercial IEMs, Neosepta® CMX and AMX were obtained from the ASTOM Corporation (Tokyo, Japan). Unless otherwise specified, special solvents and reagents purchased from Sigma-Aldrich Japan Co., Ltd. (Tokyo, Japan), Nacalai Tesque, Inc. (Kyoto, Japan) and Wako Pure Chemical Industries, Ltd. (Tokyo, Japan) were used as they were. Figure 2 shows the Chemical structure of PVA, AP-2, and PDADMAC polymers used in this study.
7
Development of Pesticide Nanoemulsion Formulations
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Polylactide (PLA) was kindly provided by Dongguan Zhuyou Plastic Co., Ltd (Dongguan, China). Bovine serum albumin (BSA) was obtained from Beijing Biodee Biotechnology Co., Ltd (Beijing, China). Commercial avermectin (Av) (18 g L−1) was purchased from Shandong Zouping Pesticide Co., Ltd. Commercial chlorantraniliprole (CAP) (200 mg mL−1) was purchased from Shanghai Shengnong Pesticide Co., Ltd. Technical Av (94.3%) and CAP (95%) were purchased from Qilu Pharmaceutical (Inner Mongolia) Co., Ltd (Huhhot, China) and DuPont Co., Ltd, respectively. Poly(vinyl alcohol) (PVA) with a Mw of 30 000–70 000 and a hydrolysis of 87–89% was purchased from Aldrich. The specification of the dialysis membrane purchased from Beijing Tianan Technology Co., Ltd, (Beijing, China). The fast membrane emulsification equipment (FMEM-500M) and Shirasu Porous Glass (SPG) membrane were purchased from National Engineering Research Center for Biotechnology. The SPG membranes were annulus cylinders with pore sizes of 7.0 μm. Other chemical reagents were of analytical grade and purchased from Beijing Chemical Works (Beijing, China).
8
Synthesis and Characterization of Ruthenium Nanoparticles
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Ru(NO)(NO3)3 solution (1.5 wt% Ru) was purchased from STREM Chemicals. Sodium borohydride and citric acid were provided by Acros Organics, and sodium hydrogenocarbonate by Aldrich Chemicals (Saint Quentin-Fallavier, France), and they were used without any further purification. Poly(citric acid-β-cyclodextrin), PCD, was synthesized by polyesterification in the presence of citric acid according to a previously reported procedure.25 (link) Poly(vinyl alcohol), PVA, was purchased from Aldrich Chemicals, 87–89% hydrolyzed, LMW = 31–50 kg mol−1, MMW = 85–124 kg mol−1 and HMW = 146–186 kg mol−1 (respectively named LMW-PVA, MMW-PVA and HMW-PVA), and used as received. Purified deionized water was purchased from Fresenius Kabi.
9
Doxorubicin-Loaded Polymeric Nanoparticles
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Polycaprolactone (Mw. 10 000 Da), polyvinyl alcohol (PVA) (Mw: 13,000–23,000 Da), potassium phosphate dibasic, and ethyl acetate were obtained from (Aldrich, UK).
Doxorubicin.HCl solution was purchased from (Ebewe Pharma, Australia). Dichloromethane (DCM) was from (Carlo, UK), Polyethylene glycol (PEG) (Mw: 8000 Da) was from (Fisher, USA), sodium chloride was from (Fluka, Germany), potassium chloride was from (Sigma, Germany), and sodium phosphate monobasic was from (Biobasic Company, Canada). The normal Vero cell line and liver cancer HepG2 cell line were purchased from the tissue culture laboratory of (VACSERA, Egypt). The 3-(4, 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide (MTT) was from (Sigma, USA), phosphate buffer saline (PBS) was from (Biowest Nuaille, France), DMEM Earle’s medium with L-Glutamine, HEPES and Pen-Strep antibiotics were purchased from (Lonza, Switzerland). Dimethylsulfoxide (DMSO) was purchased from (Sigma, Germany).
Doxorubicin.HCl solution was purchased from (Ebewe Pharma, Australia). Dichloromethane (DCM) was from (Carlo, UK), Polyethylene glycol (PEG) (Mw: 8000 Da) was from (Fisher, USA), sodium chloride was from (Fluka, Germany), potassium chloride was from (Sigma, Germany), and sodium phosphate monobasic was from (Biobasic Company, Canada). The normal Vero cell line and liver cancer HepG2 cell line were purchased from the tissue culture laboratory of (VACSERA, Egypt). The 3-(4, 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide (MTT) was from (Sigma, USA), phosphate buffer saline (PBS) was from (Biowest Nuaille, France), DMEM Earle’s medium with L-Glutamine, HEPES and Pen-Strep antibiotics were purchased from (Lonza, Switzerland). Dimethylsulfoxide (DMSO) was purchased from (Sigma, Germany).
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