Ascentis rp amide

Manufactured by Merck Group

Sourced in United States, Germany

The Ascentis RP-Amide is a reversed-phase high-performance liquid chromatography (HPLC) column. It is designed for the separation and analysis of a wide range of compounds, including polar, nonpolar, and slightly basic analytes. The column features a proprietary bonded amide stationary phase that provides excellent peak shape and good retention of polar compounds.

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Lab products found in correlation

Agilent 6510 q tof apparatus, by Agilent Technologies (3 mentions) Supelcosil lc si, by Merck Group (3 mentions) Agilent 1260 infinity 2, by Agilent Technologies (3 mentions) 1260 series pump, by Agilent Technologies (2 mentions) Synergi fusion rp, by Phenomenex (2 mentions) Amx 500, by Bruker (2 mentions) C18 reverse phase column, by Phenomenex (1 mentions) Agilent 1100, by Agilent Technologies (1 mentions) Kinetex pfp 100 a, by Phenomenex (1 mentions) Dionex ultimate 3000 system, by Thermo Fisher Scientific (1 mentions) Dionex ultimate 3000 rs, by Thermo Fisher Scientific (1 mentions) Dionex ultimate 3000, by Thermo Fisher Scientific (1 mentions) Diaion hp 20, by Merck Group (1 mentions) Silica gel, by Merck Group (1 mentions) Avance 3 700 ft nmr, by Bruker (1 mentions) 6410 triple quad lc ms, by Agilent Technologies (1 mentions) 343 polarimeter, by PerkinElmer (1 mentions) Seppak cartridge, by Waters Corporation (1 mentions) Rf 20a, by Shimadzu (1 mentions)

Spelling variants of this product

Ascentis Express RP-Amide (8 citations) Ascentis Express RP-Amide column (6 citations) Ascentis RP-Amide column (5 citations) Ascentis® Express 2.7 μ RP-Amide column (3 citations) Ascentis Express RP-amide C16 column (2 citations) Discovery Ascentis RP-Amide (2 citations) Ascentis Express RP-Amide analytical column (2 citations)

11 protocols using ascentis rp amide

Quantification of Organic Micropollutants by HPLC

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After the adsorption experiments, the concentrations of the selected pollutants were determined by High Performance Liquid Chromatography (HPLC) using a liquid chromatograph Agilent 1100 equipped with a UV detector. The stationary phase was an Ascentis RP-Amide 5 µm of 150 mm x 4.6 mm (Sigma-Aldrich, Steinheim, Germany) equipped with a pre-column Ascentis RP-Amide 5 µm of 20 mm x 4.0 mm (Sigma-Aldrich). Samples (20 µL) were manually injected into the mobile phase, which consisted of a mixture of acetonitrile/ultrapure water (0.1% H 3 PO 4 ) (55:45, v/v), at a flow rate of 1.0 mL/min. The detection wavelength was fixed at 220nm for atrazine, simazine, carbamazepine and estrone; 254nm for the parabens and 278nm for bisphenol A.
Peak identification was performed by comparison with retention times of standard solutions.
The linearity of the calibration curves was verified by triplicate analysis of standard solutions containing 0.05, 0.1, 0.3, 0.5, 1.0, 3.0 and 5.0 mg/L for atrazine, simazine, carbamazepine, estrone and parabens. Table 1 shows the coefficient of determination (r 2 ) and limits of detection (LOD) and quantification (LOQ) for each compound. Linearity was checked by means of the lack of fit test in all cases through the manuscript.

López-Ortiz C.M., Sentana-Gadea I., Varó-Galvañ P.J., Maestre-Pérez S.E, & Prats-Rico D. (2018). Effect of magnetic ion exchange (MIEX^®) on removal of emerging organic contaminants. Chemosphere, 208.

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Radio-HPLC Purification and Analysis

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Solvents and starting material
were obtained from commercial sources and were used as received. Radio-HPLC
was performed with a 1260 series pump (Agilent Technologies, Stuttgart,
Germany) with a built-in UV detector operated at 254 nm and a radioactivity
detector with a single-channel analyzer (labLogic) using a semipreparative
C18 reverse-phase column (00G-4041-N0: Luna 5 μm, 250 ×
10 mm, Phenomenex) and an analytical C18 column (5 μm, 150 ×
4.6 mm, ASCENTIS RP-AMIDE, Sigma). An acetonitrile/ammonium acetate
buffer (MeCN/NH₄OAc: 20 mM) solvent was used for quality
control analyses at a flow of 1 mL/min. R-TLC was performed using
TLC aluminum sheets and silica gel 60 matrix with fluorescent indicator.

Turkman N., Xu S., Huang C.H., Eyermann C., Salino J, & Khan P. (2023). High-Contrast PET Imaging with [18F]NT160, a Class-IIa Histone Deacetylase Probe for In Vivo Imaging of Epigenetic Machinery in the Central Nervous System. Journal of Medicinal Chemistry, 66(8), 5611-5621.

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Analytical Characterization of Compounds

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Solvents and starting material were obtained from commercial sources and were used as received. High-Performance Liquid Chromatography (HPLC) was performed with a 1260 series pump (Agilent Technologies, Stuttgart, Germany) with a built-in UV detector operated at 250 nm and a radioactivity detector with a single-channel analyzer (labLogic) using a semipreparative C18 reverse-phase column (10 × 250 mm, Phenomenex) and an analytical C18 column (4.6 × 250 mm, ASCENTIS RP-AMIDE, Sigma). An acetonitrile/ammonium acetate buffer (MeCN/NH4OAc: 20 mM) or acetonitrile/water (MeCN/water) solvents with varying composition (solvent systems were developed specific to each compound) was used for quality control analyses at a flow of 1 mL/min. High resolution mass spectroscopy (HRMS) was performed using Agilent 1260HPLC/G6224A-TOF MS. NMR spectroscopy was performed using 400 MHz Bruker instrument.

Turkman N., Liu D, & Pirola I. (2021). Novel late-stage radiosynthesis of 5-[18F]-trifluoromethyl-1,2,4-oxadiazole (TFMO) containing molecules for PET imaging. Scientific Reports, 11, 10668.

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Analytical Techniques for Compound Characterization

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Optical rotations were measured on a JASCO P-2000 spectropolarimeter (Easton, MD, USA) at 20 °C in MeOH with Spectramanager software.
HPLC used configuration of analytical system by Agilent 1100 Series (Degasser G1322A, Quaternary Pump G1311A, Autosampler ALS G1313A, Column Compartment G1316, DAD G1315B, Loop 20 µL, UV spectrum 200–900 nm) with column Kinetex^® PFP 100 A, 250 mm × 4.6 mm I.D., 5 µm (Phenomenex, CA, USA), and flow rate of 1 mL/min. Semi-preparative HPLC was carried out using Dionex UltiMate 3000 system (Pump Dionex UltiMate 3000 UPLC+ Focused, Dionex UltiMate 3000 RS Variable Wavelength Detector, fraction collector Dionex UltiMate 3000 with 6 positions, LCO 101 ECOM column oven, constant temperature 40 °C, autosampler Dionex UltiMate 3000, loop 100 µL), column Ascentis^® RP-AMIDE, 250 mm × 10 mm, 5 µm (Supelco, PA, USA), and flow rate of 5 mL/min. TLC was carried out on precoated Silica gel plates (Supelco Kieselgel G, F254, 60, Merck, Darmstadt, Germany) with the solvent systems EtOAc:MeOH:H₂O (100:13.5:10, v/v/v). Spots were visualized under UV light (365 nm) after spraying with NTS/PEG reagent. Column chromatography (CC) was performed using Diaion HP-20 (Supelco, PA, USA), Ø = 80 mm, height 70 cm ~ 700 g and Silica gel (40−63 μm, Sigma-Aldrich^®, St. Louis, MO, USA) Ø = 35 mm, height 60 cm.

Vasilev H., Ross S., Šmejkal K., Maršík P., Jankovská D., Havlík J, & Veselý O. (2019). Flavonoid Glycosides from Endemic Bulgarian Astragalus aitosensis (Ivanisch.). Molecules, 24(7), 1419.

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Characterization of Organic Compounds

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Specific rotation, PerkinElmer 343 Polarimeter (PerkinElmer, Waltham, MA, USA); NMR, Bruker Avance III 700 Bruker FT-NMR (Bruker BioSpin GmbH, Rheinstetten, Germany) (700.00/176.03 MHz) (¹H/¹³C) spectrometer; ESI MS (positive and negative ion modes), Agilent 6510 Q-TOF apparatus (Agilent Technology, Santa Clara, CA, USA), sample concentration 0.01 mg/mL; HPLC, Agilent 1260 Infinity II with a differential refractometer (Agilent Technology, Santa Clara, CA, USA); columns Supelcosil LC-Si (4.6 × 150 mm, 5 μm) and Ascentis RP-Amide (10 × 250 mm, 5 μm) (Supelco, Bellefonte, PA, USA), Phenomenex Synergi Fusion RP (10 × 250 mm, 5 μm) (Phenomenex, Torrance, CA, USA).

Silchenko A.S., Kalinovsky A.I., Avilov S.A., Andrijaschenko P.V., Popov R.S., Dmitrenok P.S., Chingizova E.A, & Kalinin V.I. (2021). Unusual Structures and Cytotoxicities of Chitonoidosides A, A1, B, C, D, and E, Six Triterpene Glycosides from the Far Eastern Sea Cucumber Psolus chitonoides. Marine Drugs, 19(8), 449.

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Quantification of Auxin Compounds by HPLC-MS

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Auxs fraction was eluted from SPE column with methanol, evaporated to dryness and reconstituted in 50 μl methanol. Samples prepared in this manner were analysed on HPLC column Supelco Ascentis RP-Amide (7.5 cm × 4.6 mm, 2.7 μm). Mobile phases were 0.1 % formic acid solution in water (solvent A) and acetonitrile/methanol (1/1) mixture. Gradient elution was applied under the flow rate of 0.5 ml/min. HPLC apparatus was Agilent Technologies 1260 equipped with Agilent Technologies 6410 Triple Quad LC/MS with ESI (Electrospray Interface). Two most abundant secondary ions were monitored (MRM—multiple reaction monitoring mode). One of them was used for quantification, whereas the second was used for additional confirmation of identity. The monitored ions were: indole-3-acetic acid (IAA)—m/z 176.1 primary, 130.3, 77.2 secondary; indolebutyric acid (IBA)—m/z 204.1 primary, 186.4, 130.3 secondary; D-IAA (deuterated IAA used as internal standard)—m/z 181.1 primary, 134.7, 81.4 secondary.

Żur I., Dubas E., Krzewska M., Waligórski P., Dziurka M, & Janowiak F. (2014). Hormonal requirements for effective induction of microspore embryogenesis in triticale (× Triticosecale Wittm.) anther cultures. Plant Cell Reports, 34(1), 47-62.

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Spectroscopic Analysis of Chemical Compounds

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We used specific rotation with a PerkinElmer 343 Polarimeter (PerkinElmer, Waltham, MA, USA); NMR, Bruker AMX 500 (Bruker BioSpin GmbH, Rheinstetten, Germany) (500.12/125.67 MHz (¹H/¹³C) spectrometer; ESI MS (positive and negative ion modes), Agilent 6510 Q-TOF apparatus (Agilent Technology, Santa Clara, CA, USA)), sample concentration 0.01 mg/mL; HPLC, Agilent 1260 Infinity II with a differential refractometer (Agilent Technology, Santa Clara, CA, USA); and columns Supelcosil LC-Si (4.6 × 150 mm, 5 µm) and Ascentis RP-Amide (10 × 250 mm, 5 µm) (Supelco, Bellefonte, PA, USA) and Phenomenex Synergi Fusion RP (10 × 250 mm, 5 µm) (Phenomenex, Torrance, CA, USA).

Silchenko A.S., Avilov S.A., Andrijaschenko P.V., Popov R.S., Chingizova E.A., Dmitrenok P.S., Kalinovsky A.I., Rasin A.B, & Kalinin V.I. (2022). Structures and Biologic Activity of Chitonoidosides I, J, K, K1 and L-Triterpene Di-, Tri- and Tetrasulfated Hexaosides from the Sea Cucumber Psolus chitonoides. Marine Drugs, 20(6), 369.

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Quantification of Urinary 1-Aminopyrene

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Urinary 1-AP levels were measured from 2 h urine samples. One milliliter of 10 M hydrochloric acid was added to each 10 mL urine sample, and the urine was stirred in a 90-°C water bath for 2 h. After adjusting the pH to 7.0–8.0, the supernatant was extracted with Sep-Pak cartridge (C18, 3 mL, 200 mg, Waters, Milford, UK). Before extraction, it was pre-conditioned with 5 mL of methanol and 5 mL of water. After loading the sample, it was washed with 5 mL of 20% methanol in water and then extracted with 4 mL of 100% methanol. The extract was dried with N₂ gas and dissolved in 200 μL of methanol. The extract was analyzed by injecting 20 μL into the HPLC system equipped with a fluorescence detector (Shimadzu, RF-20A, Kyoto, Japan). A reverse phase-amide column (Ascentis RP-Amide, 25 cm × 4.6 mm, 5 μm, SUPELCO, Bellefonte, PA, USA) was used. The mobile phase was composed of methanol and 50 mM sodium acetate buffer pH 7.2 (80:20, v/v) at a flow rate of 1.0 mL/min, and the excitation and emission wavelengths were 254 nm and 425 nm, respectively (Fig. 1). Urinary 1-AP concentration was corrected using the urinary creatinine level.Fig. 1

Chromatograms for 1-aminopyrene (a 1-aminopyrene standard 10 ng/ml, b urine sample)

Ochirpurev B., Eom S.Y., Toriba A., Kim Y.D, & Kim H. (2021). Urinary 1-aminopyrene level in Koreans as a biomarker for the amount of exposure to atmospheric 1-nitropyrene. Toxicological Research, 38(1), 45-51.

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Inotodiol Purity Analysis via HPLC

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Inotodiol purity was analyzed using an Agilent 1100 chromatograph equipped with a differential refractometer on reversed phase column Supelco Ascentis RP-Amide (10 × 250 mm, 5 µm) with methanol as the mobile phase (flow rate, 2 mL/min). Inotodiol was dissolved in dimethylsulfoxide (DMSO) and stock solutions were diluted with DC culture medium.

Maza P.A., Lee J.H., Kim Y.S., Sun G.M., Sung Y.J., Ponomarenko L.P., Stonik V.A., Ryu M, & Kwak J.Y. (2021). Inotodiol From Inonotus obliquus Chaga Mushroom Induces Atypical Maturation in Dendritic Cells. Frontiers in Immunology, 12, 650841.

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Comprehensive Analytical Characterization

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Specific rotation, PerkinElmer 343 Polarimeter (PerkinElmer, Waltham, MA, USA); NMR, Bruker AMX 500 (Bruker BioSpin GmbH, Rheinstetten, Germany) (500.12/125.67 MHz (¹H/¹³C) spectrometer; ESI MS (positive and negative ion modes), Agilent 6510 Q-TOF apparatus (Agilent Technology, Santa Clara, CA, USA), sample concentration 0.01 mg/mL; HPLC, Agilent 1260 Infinity II with a differential refractometer (Agilent Technology, Santa Clara, CA, USA); columns Supelcosil LC-Si (4.6 × 150 mm, 5 µm) and Ascentis RP-Amide (10 × 250 mm, 5 µm) (Supelco, Bellefonte, PA, USA).

Silchenko A.S., Kalinovsky A.I., Avilov S.A., Andrijaschenko P.V., Popov R.S., Chingizova E.A., Kalinin V.I, & Dmitrenok P.S. (2021). Triterpene Glycosides from the Far Eastern Sea Cucumber Psolus chitonoides: Chemical Structures and Cytotoxicities of Chitonoidosides E1, F, G, and H. Marine Drugs, 19(12), 696.

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