H e staining kit

Manufactured by Keygen Biotech

Sourced in China

The H&E staining kit is a laboratory product used for the histological staining of tissue samples. It contains the necessary reagents and protocols to perform hematoxylin and eosin (H&E) staining, a common technique in pathology and histology. The kit allows for the visualization and differentiation of various cellular and tissue structures under a microscope.

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Lab products found in correlation

Rpmi 1640 cell culture medium, by Thermo Fisher Scientific (2 mentions) Reactive oxygen species detection kit, by Beyotime (2 mentions) Cell cycle detection kit, by Beyotime (2 mentions) Protease inhibitor, by Boster Bio (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (2 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (2 mentions) Anti ki67, by Wuhan Servicebio Technology (2 mentions) Tunel reaction solution, by Boster Bio (2 mentions) Annexin 5 fitc pi apoptosis detection reagent, by BD (2 mentions) Membrane 2 kd, by Solarbio (2 mentions) Anti caspase 9, by Wuhan Servicebio Technology (2 mentions) Anti caspase 3, by Wuhan Servicebio Technology (2 mentions) Cell lysate, by Boster Bio (2 mentions) Anti p53, by Wuhan Servicebio Technology (2 mentions) Dm3000, by Leica (1 mentions) Wheat germ agglutinin (wga), by Merck Group (1 mentions) Deadend fluorometric tunel system, by Promega (1 mentions) Hoechst 33342, by Keygen Biotech (1 mentions) P akt, by Cell Signaling Technology (1 mentions)

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H&E staining (2 citations)

8 protocols using h e staining kit

Quantifying Muscle Fiber Morphology and Apoptosis

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Hematoxylin and eosin (H&E)-stained and wheat germ agglutinin (WGA) staining were used to determine the cross-sectional area of myofibers. 10 μm thickness paraffin sections were subjected to H&E staining using an H&E staining kit (KeyGEN) according to published protocols. 10 μm cryosections were subjected to WGA staining using standard protocols. WGA (1:100; Sigma) and Hoechst 33342 (1:2000, KeyGEN) were used to label the cell membrane and nucleus, separately. The images of the sections were taken with microscope Leica (Wetzlar, Germany, DM3000). Cross-sectional areas of myofibers were measured by using ImageJ software.
TdT mediated dUTP nick end labeling (TUNEL) staining was used to detect and quantitate apoptotic myofibers. 10 μm thick frozen sections were subjected to DeadEnd Fluorometric TUNEL System (Promega) according to standard protocols. Images were taken by Confocal Imaging Microscope System (20× magnification) (Olympus). TUNEL-positive cells (FITC) and all cells (DAPI) were counted in a slide.

Liu Q., Yuan W., Yan Y., Jin B., You M., Liu T., Gao M., Li J., Gokulnath P., Vulugundam G., Li G., Xu B, & Xiao J. (2023). Identification of a novel small-molecule inhibitor of miR-29b attenuates muscle atrophy. Molecular Therapy. Nucleic Acids, 31, 527-540.

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Histological Analysis of Ocular Tissue

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After the irritation test was completed, H&E staining was performed to investigate the safety of the gelling in vivo. Corneas were carefully dissected and washed twice with physiological saline. The samples were then fixed in 4% formaldehyde. The fixed samples were dehydrated using different gradient concentrations of alcohol. Then, ocular tissues were embedded in paraplast plus and cut into 5-μm-thick sections. Histological sections were fixed in 4% paraformaldehyde, and then stained using the KeyGEN H&E staining kit according to the kit instructions.

Xu S., Ke L., Zhao S., Li Z., Xiao Y., Wu Y., Ren J, & Qiu Y. (2021). Thermosensitive Poly(DHSe/PEG/PPG Urethane)-Based Hydrogel Extended Remdesivir Application in Ophthalmic Medication. Pharmaceutics, 14(1), 50.

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Molecular Mechanisms of Alzheimer's Disease

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Erzhi pills were purchased from Jiangxi Yaodu Zhangshu Pharmaceutical Co., Ltd. (Jiangxi, China). Specnuezhenide (purity > 98%) was obtained from Jiangxi Bencao Tiangong Biotechnology Co., Ltd. (Jiangxi, China). Oestradiol valerate tablets were purchased from the Guangzhou branch of Bayer (Guangzhou, China). The rat ELISA kit was supplied by Andy Gene Biotechnology Co., Ltd. (Beijing, China). The H&E staining kit and Nissl staining kit were obtained from Jiangsu KeyGEN BioTECH Co., Ltd. (Jiangsu, China). The SP Rabbit HRP (DAB) immunohistochemical kit was obtained from Jiangsu Kang Wei Shi Ji Biotechnology Co., Ltd. (Jiangsu, China). dl-Dithiothreitol, RIPA, and PMSF were obtained from Thermo Fisher Scientific Inc. (USA). Aβ_1–40, d-galactose, iodoacetamide, acetone, formic acid, trifluoro acetic acid, and acetic acid were supplied by Sigma Aldrich Co. (St. Louis, MO, USA). Sequencing trypsin was purchased from Promega Corporation (Madison, WI, USA). Anti Aβ_1–40, p-tau⁴⁰⁴, GSK3β, p-GSK3β, 14-3-3β/α, Bad, Bcl-xl, and Bcl-2 antibodies were obtained from Abcam Inc. (Cambridge, UK). p-Akt, Akt, p-PI3K, PI3K, and anti-β-actin antibodies were produced by Cell Signalling Technology (Danvers, MA, USA). All other chemicals were of analytical grade.

Xie Y., Yan B., Hou M., Zhou M., Liu C., Sun M., He K., Fang C., Chen Y, & Huang L. (2021). Erzhi pills ameliorate cognitive dysfunction and alter proteomic hippocampus profiles induced by d-galactose and Aβ1–40 injection in ovariectomized Alzheimer’s disease model rats. Pharmaceutical Biology, 59(1), 1400-1412.

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Histological Examination of Synovial Tissue

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Synovial tissue sections were dewaxed with 4% xylene and dehydrated with gradient alcohol.31 (link) For H&E staining, tissues were stained with H&E staining kit (toluidine blue, Keygen Biotech. Nanjing, China). For Masson, tissues were stained with Masson trichrome staining kit (toluidine blue, Keygen Biotech. Nanjing, China). For Safranin O staining, tissues were stained with Safranin O solution (ScyTek, Logan, UT, USA). All staining was performed according to the manufacturer’s instruction, and the morphological changes were observed with an optical microscope (BX51; Olympus Corp., Tokyo, Japan).

Gao H., Peng L., Li C., Ji Q, & Li P. (2020). Salidroside Alleviates Cartilage Degeneration Through NF-κB Pathway in Osteoarthritis Rats. Drug Design, Development and Therapy, 14, 1445-1454.

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Mesoporous Silica Nanoparticle Anticancer Assay

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Mesoporous silica dispersion was purchased from nanoComposix Company (USA), and Isoimperatorin from DESITE (China). Fetal bovine serum and RPMI1640 cell culture medium were extracted by Gibco (USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo laboratories (Japan), and the Annexin V-FITC/PI apoptosis detection reagent was produced by BD (USA). Cell cycle detection kit, reactive oxygen species (ROS) detection kit, and DAPI were acquired from Beyotime Biotechnology (China). Rhodamine 123 (Rh123) was provided by Yeasen Biotechnology (China). Cy5 and membrane (2 kD) were purchased from Solarbio (China). Cell lysate, Protease inhibitor and TUNEL reaction solution were obtained from Boster (China). Anti-Ki-67, anti-P53, anti-Caspase-9 and anti-Caspase-3 antibodies were all purchased from Servicebio (China), while anti-Pan-Cadherin and anti-E-Cadherin antibodies were acquired from CST (USA). H&E staining kit was purchased from keygen BioTECH (Chnia), and human lymphoma cell line OCI-LY10 was acquired from the Shanghai Institute of Cell Research, Chinese Academy of Sciences.

Zhao Q., Sun X., Wu B., Shang Y., Huang X., Dong H., Liu H., Chen W., Gui R, & Li J. (2021). Construction of homologous cancer cell membrane camouflage in a nano-drug delivery system for the treatment of lymphoma. Journal of Nanobiotechnology, 19, 8.

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Histological Analysis of Bone Formation

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Sections were stained with an H&E Staining Kit (KeyGen) and TRAP staining kit (Sigma-Aldrich). An alkaline phosphatase (ALP) staining kit (Jiancheng) was utilized to detect bone formation. For immunofluorescence staining, the primary antibodies used were rabbit anti-HIF-1α (1:200; Abcam) and mouse anti-CTSK (1:200; BBI). Then, the slides were incubated with DyLight 488-conjugated goat anti-mouse IgG (1:500; Abbkine) or DyLight 594-conjugated goat anti-rabbit IgG (1:500; Abbkine) followed by DAPI (1:800; Sigma-Aldrich).

Tian Y., Shao Q., Gu J., Tang Y., Bie M., Zhou Y., Cheng C., Liang Y., Zhang Q, & Kang F. (2022). LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1α. Frontiers in Genetics, 13, 909095.

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IHC Staining of Tissue Samples

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IHC was performed as describe previously (16 (link)). Briefly, the tissues were exposed to primary antibodies like macrophage inflammatory protein-1α (CCL3; 1:200, DF8572, Affinity), MMP3 Monoclonal antibody (1:200, 66338-1-Ig, Proteintech), and TIMP1 polyclonal antibody (1:200, 16644-1-AP, Proteintech). The tissues were stained with hematoxylin-eosin (HE) using the HE staining Kit (KGA224, keyGEN BioTECH, Nanjing, Jiangsu).

Pan Z., Lin H., Fu Y., Zeng F., Gu F., Niu G., Fang J, & Gu B. (2023). Identification of gene signatures associated with ulcerative colitis and the association with immune infiltrates in colon cancer. Frontiers in Immunology, 14, 1086898.

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Mesoporous Silica Nanoparticle Cytotoxicity

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Mesoporous silica dispersion was purchased from nanoComposix Company (USA), and Isoimperatorin from DESITE (China). Fetal bovine serum and RPMI1640 cell culture medium were extracted by Gibco (USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo laboratories (Japan), and the Annexin V-FITC/PI apoptosis detection reagent was produced by BD (USA). Cell cycle detection kit, reactive oxygen species (ROS) detection kit, JC-1 detection kit and DAPI were acquired from Beyotime Biotechnology (China). Cy5 and membrane (2 kD) were purchased from Solarbio (China). Cell lysate, Protease inhibitor and TUNEL reaction solution were obtained from Boster (China). Anti-Ki-67, anti-P53, anti-Caspase-9 and anti-Caspase-3 antibodies were all purchased from Servicebio (China), while anti-Pan-Cadherin and anti-E-Cadherin antibodies were acquired from CST (USA). H&E staining kit was purchased from keygen BioTECH (Chnia), and human lymphoma cell line OCI-LY10 was acquired from the Shanghai Institute of Cell Research, Chinese Academy of Sciences.

Zhao Q., Song Y., Wu B., Shang Y., Huang X., Dong H., Liu H., Chen W., Gui R., & J L. (2020). Construction of homologous cancer cell membrane camouflage in a nano-drug delivery system for the treatment of lymphoma.

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