Ly 6g

Manufactured by Merck Group

Ly-6G is a cell surface marker that is primarily expressed on the surface of neutrophils, a type of white blood cell. It is commonly used in flow cytometry and other immunological assays to identify and quantify neutrophil populations in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Cd11b, by Merck Group (2 mentions) Luminex mouse 32 plex mcytmag 70k px32, by Merck Group (2 mentions) Zombie nir, by BioLegend (2 mentions) Cd11c pe cy7, by BioLegend (2 mentions) Ly6c fitc, by BioLegend (2 mentions) Ic fixation buffer, by Thermo Fisher Scientific (2 mentions) Ly6g bv650, by BioLegend (1 mentions)

2 protocols using ly 6g

Comprehensive Immune Characterization of BALF

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BAL fluid was collected from each animal through cannulation of the exposed trachea, and flushing twice with .7mL of PBS. BALF samples were centrifuged at 500xG for 5 minutes, and supernatant was immediately frozen for later cytokine analyses. For flow cytometry, pelleted cells were resuspended in FACS (PBS + 5% FBS) buffer for staining and with Zombie NIR (Biolegend, 423105, SanDiego, CA), CD45, Alexa Fluor 532 (eBioscience, 58-0459-42,San Diego, CA), CD11c, PE-Cy7 (Biolegend, 117317, SanDiego, CA), CD11B, BV480 (BD Biosciences, 566117, SanJose, CA), SIGLEC F, AF700 (eBioscience, 56-1702-80, SanDiego, CA), Ly-6c, FITC (Biolegend, 128005, San Diego, CA),and Ly-6G, BV650 (Biolegend, 127641, San Diego, CA) and then fixed in IC-fixation buffer (eBioscience, 00-8222-49, San Diego, CA). Samples were run on a Cytek Aurora Borealis at the University of Virginia flowcytometry core. Neutrophils are Zombie NIR−, CD45+,CD11C−, CD11B+, and Ly-6G+; eosinophils are Zombie NIR−,CD45+, CD11C−, CD11B+, and Siglec-F+; inflammatory monocytes are Zombie NIR−, CD45+, CD11C−, CD11B+, Ly-6G−, and Ly-6C+.
Cytokine analyses were performed via Luminex Mouse 32-plex (MCYTMAG-70K-PX32, Millipore-sigma). Samples were run following manufacturers protocol after an 18-hour incubation before being run on Luminex® analyzer (MAGPIX®).

Donlan A.N., Sutherland T.E., Marie C., Preissner S., Bradley B.T., Carpenter R.M., Sturek J.M., Ma J.Z., Moreau G.B., Donowitz J.R., Buck G.A., Serrano M.G., Burgess S.L., Abhyankar M.M., Mura C., Bourne P.E., Preissner R., Young M.K., Lyons G.R., Loomba J.J., Ratcliffe S.J., Poulter M.D., Mathers A.J., Day A., Mann B.J., Allen J.E, & Petri WA J.r. (2021). IL-13 is a driver of COVID-19 severity. medRxiv.

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Analyzing Immune Cell Subsets in BALF

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BAL fluid was collected from each animal through cannulation of the exposed trachea and flushed twice with 0.7mL of PBS. BALF samples were centrifuged at x500 g for 5 minutes, and supernatant was immediately frozen for later cytokine analyses. For flow cytometry, pelleted cells were resuspended in FACS (PBS + 5% FBS) buffer for staining and with Zombie NIR (BioLegend, 423105), CD45, Alexa Fluor 532 (eBioscience, 58-0459-42), CD11c, PE-Cy7 (BioLegend, 117317), CD11B, BV480 (BD Biosciences, 566117), SIGLEC F, AF700 (eBioscience, 56-1702-80), Ly-6c, FITC (BioLegend, 128005), and Ly-6G, BV650 (BioLegend, 127641) and then fixed in IC-fixation buffer (eBioscience, 00-8222-49). Samples were run on a Cytek Aurora Borealis at the University of Virginia flow cytometry core. Neutrophils are Zombie NIR−, CD45+, CD11C−, CD11B+, and Ly-6G+; eosinophils are Zombie NIR−, CD45+, CD11C−, CD11B+, and Siglec-F+; and inflammatory monocytes are Zombie NIR−, CD45+, CD11C−, CD11B+, Ly-6G−, and Ly-6C+.
Cytokine analyses were performed via Luminex Mouse 32-plex (MCYTMAG-70K-PX32, MilliporeSigma). Samples were run following manufacturers protocol after an 18-hour incubation before being run on Luminex analyzer (MAGPIX).

Donlan A.N., Sutherland T.E., Marie C., Preissner S., Bradley B.T., Carpenter R.M., Sturek J.M., Ma J.Z., Moreau G.B., Donowitz J.R., Buck G.A., Serrano M.G., Burgess S.L., Abhyankar M.M., Mura C., Bourne P.E., Preissner R., Young M.K., Lyons G.R., Loomba J.J., Ratcliffe S.J., Poulter M.D., Mathers A.J., Day A.J., Mann B.J., Allen J.E, & Petri WA J.r. (2021). IL-13 is a driver of COVID-19 severity. JCI Insight, 6(15), e150107.

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