Alexa fluor 488

To investigate the correlation between the stromal nerve terminals and origin of the anterior zonules the anterior halves from 9 human and 5 monkey eyes that had been fixed in PFA were dissected into four quadrants. From each quadrant small 3–5 mm wide wedge shaped specimens containing ciliary body, sclera, adjacent parts of the iris and cornea as well as lens equator were prepared and partly frozen in liquid nitrogen for the preparation of cryostat sections or embedded in paraffin wax. Serial sections were cut at 6 to 25 μm in sagittal, frontal and horizontal planes, mounted on 0.1% poly-L-lysine–coated slides and allowed to dry for several hours. After preincubation in 1% dry milk solution for 15 minutes, the sections were incubated with the primary antibody (mouse anti-fibrillin/quartett, 1:30; rabbit anti-human elastin/Millipore 1:50; rabbit anti-pan neurofilaments/Biotrend 1:400; mouse anti-synaptophysin/Dako 1:10; rabbit anti-calretinin/swant 1:2000) and appropriate Alexa Fluor 488– or Alexa Fluor 555–labelled immunoglobulin G secondary antibody (MobiTec, 1:300). For light microscopy paraffin sections were stained with Weigert’s stain. All sections were viewed with a Keyence BZ-9000 (BZ-9000/models/BZ-9000e/index.jsp">keyence.de/products/microscope/fluorescence-microscope/BZ-9000/models/BZ-9000e/index.jsp).