HK-2 cells were purchased from Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China) and was maintained (at 37°C in a humidified atmosphere of 5% CO2) in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Los Angeles, CA, USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. For high glucose challenge, HK-2 cells were cultured in DMEM medium containing various concentrations (15, 30, 45 mM) of D-glucose (Sigma-Aldrich, St. Louis, MO, USA) for 48 h.
Hk 2 cells
Manufactured by Shanghai Cell Bank
Sourced in China
HK-2) cells are a human proximal tubular epithelial cell line derived from normal adult human kidney. These cells are commonly used in research studies to model various aspects of human kidney physiology and pathology.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by Dojindo Laboratories (2 mentions)
D glucose, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hk 2 cells, by Merck Group (1 mentions)
Facsaria, by BD (1 mentions)
Lsm 510, by Zeiss (1 mentions)
Th4 200, by Olympus (1 mentions)
Fluo 4 am, by Beyotime (1 mentions)
Acridine orange, by Solarbio (1 mentions)
U hglgps, by Olympus (1 mentions)
Monodansylcadaverine mdc, by Solarbio (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Keygen Biotech (1 mentions)
Annexinv fitc propidium iodide, by Keygen Biotech (1 mentions)
Annexin 5 fitc, by Beyotime (1 mentions)
Propidium iodide, by Beyotime (1 mentions)
Lactate dehydrogenase ldh kit, by Beyotime (1 mentions)
Hematoxylin eosin dye, by Beyotime (1 mentions)
4 protocols using hk 2 cells
1
Hyperglycemic Impact on Kidney Cells
2
In Vitro Kidney Cell Culture
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Human kidney proximal
tubular epithelial (HK-2) cells were purchased from the Shanghai Cell
Bank, Chinese Academy of Sciences (Shanghai, China). Dulbecco’s
modified Eagle’s medium (DMEM) and fetal bovine serum were
purchased from HyClone Biochemical Products Co., Ltd. (UT). Cell Counting
Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan).
Lactate dehydrogenase (LDH) kit, acridine orange (AO, Sigma), 2′,7′-dichlorofluorescein
diacetate dye (DCFH-DA), hematoxylin–eosin (HE) dye, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine
iodide (JC-1), rabbit antimouse IgG conjugated with fluorescein isothiocyanate
(FITC-IgG), annexin V-FITC/propidium iodide (PI), and AO/PI were all
purchased from Shanghai Beyotime Bio-Tech Co., Ltd. (Shanghai, China).
Cell culture plates were purchased from Wuxi Nest Bio-Tech Co., Ltd.
(Wuxi, China). Calcium chloride (CaCl2), potassium oxalate
(K2Ox), trisodium citrate (Na3cit·2H2O), sodium chloride (NaCl), and the other conventional reagents
were all analytically pure and purchased from Guangzhou Chemical Reagent
Factory of China (Guangzhou, China).
tubular epithelial (HK-2) cells were purchased from the Shanghai Cell
Bank, Chinese Academy of Sciences (Shanghai, China). Dulbecco’s
modified Eagle’s medium (DMEM) and fetal bovine serum were
purchased from HyClone Biochemical Products Co., Ltd. (UT). Cell Counting
Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan).
Lactate dehydrogenase (LDH) kit, acridine orange (AO, Sigma), 2′,7′-dichlorofluorescein
diacetate dye (DCFH-DA), hematoxylin–eosin (HE) dye, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine
iodide (JC-1), rabbit antimouse IgG conjugated with fluorescein isothiocyanate
(FITC-IgG), annexin V-FITC/propidium iodide (PI), and AO/PI were all
purchased from Shanghai Beyotime Bio-Tech Co., Ltd. (Shanghai, China).
Cell culture plates were purchased from Wuxi Nest Bio-Tech Co., Ltd.
(Wuxi, China). Calcium chloride (CaCl2), potassium oxalate
(K2Ox), trisodium citrate (Na3cit·2H2O), sodium chloride (NaCl), and the other conventional reagents
were all analytically pure and purchased from Guangzhou Chemical Reagent
Factory of China (Guangzhou, China).
3
Evaluation of Cellular Responses in HK-2 Cells
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Reagents:
DMEM/F-12 culture medium and fetal bovine serum were purchased from
Gibco. The CCK-8 kit was purchased from Dojindo Laboratory (Kumamoto,
Japan). Cell membrane red fluorescent probe 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
perchlorate (DiI), 4,6-diamidino-2-phenylindole (DAPI) staining solution,
and Fluo-4 AM were purchased from Shanghai Biyuntian Biotechnology
Co., Ltd. Annexin V-FITC/propidium iodide (PI), 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi-dazolylcarbocyanine
iodide (JC-1), and 2′,7-dichlorodihydrofluorescein diacetate
(DCFH-DA) were purchased from KeyGEN BioTECH Co. Ltd. (Nanjing, China).
Acridine orange (AO) and monodansylcadaverine (MDC) were purchased
from Beijing Solarbio Technology Co., Ltd. Human kidney proximal tubular
epithelial (HK-2) cells were purchased from the Shanghai Cell Bank
of the Chinese Academy of Sciences (Shanghai, China).
Instruments:
multifunctional microplate reader (SafireZ, Tecan, Switzerland); flow
cytometer (FACS Aria, BD, USA); inverted fluorescence microscope (OLYMPUS,
U-HGLGPS, Japan); laser confocal microscope (LSM510 META DUO SCAN,
ZEISS, Germany); and optical microscope (OLYMPUS, TH4-200, Japan).
DMEM/F-12 culture medium and fetal bovine serum were purchased from
Gibco. The CCK-8 kit was purchased from Dojindo Laboratory (Kumamoto,
Japan). Cell membrane red fluorescent probe 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
perchlorate (DiI), 4,6-diamidino-2-phenylindole (DAPI) staining solution,
and Fluo-4 AM were purchased from Shanghai Biyuntian Biotechnology
Co., Ltd. Annexin V-FITC/propidium iodide (PI), 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi-dazolylcarbocyanine
iodide (JC-1), and 2′,7-dichlorodihydrofluorescein diacetate
(DCFH-DA) were purchased from KeyGEN BioTECH Co. Ltd. (Nanjing, China).
Acridine orange (AO) and monodansylcadaverine (MDC) were purchased
from Beijing Solarbio Technology Co., Ltd. Human kidney proximal tubular
epithelial (HK-2) cells were purchased from the Shanghai Cell Bank
of the Chinese Academy of Sciences (Shanghai, China).
Instruments:
multifunctional microplate reader (SafireZ, Tecan, Switzerland); flow
cytometer (FACS Aria, BD, USA); inverted fluorescence microscope (OLYMPUS,
U-HGLGPS, Japan); laser confocal microscope (LSM510 META DUO SCAN,
ZEISS, Germany); and optical microscope (OLYMPUS, TH4-200, Japan).
4
HK-2 Cell Cytotoxicity Assay Protocol
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Human kidney proximal tubular epithelial (HK-2) cells were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). Dulbecco’s modified Eagle’s medium (DMEM) and Fetal bovine serum were purchased from HyClone Biochemical Products Co., Ltd. (UT, USA). Penicillin and streptomycin were purchased from Beijing Pubo Biotechnology Co., Ltd. (Beijing, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). Lactate dehydrogenase (LDH) kit, hematoxylin–eosin (H&E) dye, 2′,7′-dichlorofluorescein diacetate dye (DCFH-DA) and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1), Annexin V-FITC, Propidium iodide (PI), and Rabbit anti-mouse IgG conjugated with fluorescein isothiocyanate (FITC-IgG) were all purchased from Shanghai Beyotime Bio-Tech Co., Ltd. (Shanghai, China). Cell culture plates were purchased from Wuxi Nest Bio-Tech Co., Ltd. (Wuxi, China). Calcium chloride (CaCl2), sodium oxalate (Na2Ox), ethylenediaminetetraacetic acid (Na2EDTA), dodecyl sodium sulfate (SDS) and the other conventional reagents were all analytically pure and purchased from Guangzhou Chemical Reagent Factory of China (Guangzhou, China).
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