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Crl 6509

Manufactured by American Type Culture Collection
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The CRL-6509 is a cell line originating from human breast tissue. It is a commonly used model system for cancer research and drug development studies.

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Lab products found in correlation

Nunc lab tek 2 cc2 chamber slide system, by Thermo Fisher Scientific (2 mentions) Vr 1238, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) A375 ma2, by American Type Culture Collection (1 mentions)

3 protocols using crl 6509

1

Optimization of KRV Infection Assay

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Subconfluent monolayers of normal rat kidney (NRK) cells (CRL-6509; American Type Culture Collection, Manassas, VA) were grown in DMEM supplemented with 10% FBS on the Nunc Lab-Tek II CC2 Chamber Slide System (Thermo Fisher Scientific) for 24 h. A total of 5 × 104 NRK cells were infected with either KRV or vesicular stomatitis virus (VSV; VR-1238; American Type Culture Collection) at a multiplicity of infection of 1 for 24 h. Cell fixation was performed with 10% neutral buffered formalin, followed by gentle rinsing with 1× PBS at room temperature. Cells were then dehydrated with serial wash steps of ethanol. The fixed, dehydrated slides were stored at −20°C until used in RNAscope ISH assays.
KRV-infected weanling WT and Ifnar1−/− rats were euthanized, and fresh spleens were harvested. Immediately following dissection, tissues were fixed in 10% neutral buffered formalin for 16-32 h at room temperature. Paraffin-embedded sections were sectioned at 5 μm in the UMass Medical School Morphology Core laboratory (www.umassmed.edu/morphology/protocols).
Qaisar N., Arowosegbe A., Derr A.G., Kucukural A., Satish B., Racicot R., Guo Z., Trombly M.I, & Wang J.P. (2021). Type I IFN–Driven Immune Cell Dysregulation in Rat Autoimmune Diabetes. ImmunoHorizons, 5(10), 855-869.
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2

Culturing Human Melanoma and Rat Kidney Cells

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Human melanoma cells lines A375-P (CRL-3224) and A375-MA2 (CRL-3223) [49 (link)], and Normal Rat Kidney (NRK, CRL-6509) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were cultured in Dulbecco’s Modified Eagle Medium 1X (DMEM 1X) containing 4.5 g/L d-glucose, l-glutamine, 110 mg/L sodium pyruvate, 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific; Waltham, MA, USA), 100 units/mL penicillin, and 0.1 mg/mL streptomycin. All cells were incubated at 37 °C at 5% CO2. Trypsin (0.25%, 1 mM EDTA 1X; (Gibco, Thermo Fisher Scientific) was used to dissociate cells from culture dishes. Exogenous expression of human PANX1 in NRK cells was conducted as previously described [45 (link)], and used as a positive control.
Freeman T.J., Sayedyahossein S., Johnston D., Sanchez-Pupo R.E., O’Donnell B., Huang K., Lakhani Z., Nouri-Nejad D., Barr K.J., Harland L., Latosinsky S., Grant A., Dagnino L, & Penuela S. (2019). Inhibition of Pannexin 1 Reduces the Tumorigenic Properties of Human Melanoma Cells. Cancers, 11(1), 102.
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3

Optimization of KRV Infection Assay

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Subconfluent monolayers of normal rat kidney (NRK) cells (CRL-6509; American Type Culture Collection, Manassas, VA) were grown in DMEM supplemented with 10% FBS on the Nunc Lab-Tek II CC2 Chamber Slide System (Thermo Fisher Scientific) for 24 h. A total of 5 × 104 NRK cells were infected with either KRV or vesicular stomatitis virus (VSV; VR-1238; American Type Culture Collection) at a multiplicity of infection of 1 for 24 h. Cell fixation was performed with 10% neutral buffered formalin, followed by gentle rinsing with 1× PBS at room temperature. Cells were then dehydrated with serial wash steps of ethanol. The fixed, dehydrated slides were stored at −20°C until used in RNAscope ISH assays.
KRV-infected weanling WT and Ifnar1−/− rats were euthanized, and fresh spleens were harvested. Immediately following dissection, tissues were fixed in 10% neutral buffered formalin for 16-32 h at room temperature. Paraffin-embedded sections were sectioned at 5 μm in the UMass Medical School Morphology Core laboratory (www.umassmed.edu/morphology/protocols).
Qaisar N., Arowosegbe A., Derr A.G., Kucukural A., Satish B., Racicot R., Guo Z., Trombly M.I, & Wang J.P. (2021). Type I IFN–Driven Immune Cell Dysregulation in Rat Autoimmune Diabetes. ImmunoHorizons, 5(10), 855-869.
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