Blood dna extraction qiaamp dna mini kit

Manufactured by Qiagen

Sourced in Germany

The QIAamp® DNA Mini Kit is a laboratory tool designed for the extraction and purification of DNA from blood samples. It utilizes a silica-based membrane technology to efficiently capture and concentrate DNA, enabling its separation from other cellular components. The kit provides a standardized protocol and the necessary reagents to facilitate a reliable and consistent DNA extraction process.

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2 protocols using blood dna extraction qiaamp dna mini kit

Blood and Amniotic Fluid DNA Extraction

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DNA was extracted from blood or amniotic fluid by Blood DNA Extraction QIAamp® DNA Mini Kit (Cat No./ID: 51304, QIAGEN) following the optimal instruction from QIAGEN Producer. Purified DNA was qualified and quantified by NanoDrop One Spectrophotometer with criteria: OD A260/A280 between 1.7-2.0 and concentration above 10 ng/µl.

Truong D.T., Minh N.V., Nhung D.P., Luong H.V., Quyet D., Anh T.N., Son T.T., Tung N.T., Ha N.T., Anh D.T., Hoang L., Thuy N.L., Hoa N.T., Bac N.D., Nga V.T, & Dinh T.C. (2019). Short Tandem Repeats Used in Preimplantation Genetic Testing of Β-Thalassemia: Genetic Polymorphisms For 15 Linked Loci in the Vietnamese Population. Open Access Macedonian Journal of Medical Sciences, 7(24), 4383-4388.

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Genotyping of Healthy Females and HEMA Carriers

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Blood samples were collected from 50 unrelated healthy females and 103 HEMA carriers at the National Institute of Hematology and Blood Transfusion (NIHBT) in Hanoi, Vietnam from September 2018 to June 2019. DNA extraction was performed using the Blood DNA Extraction QIAamp® DNA Mini Kit (Qiagen, Hilden, Germany) following manufacturer’s protocol. Extracted DNAs were genotyped for STR markers to determine markers’ heterozygosity values. This study was approved by the Ethics Council in Biomedical Research of Vietnam Military Medical University. Written informed consent was obtained from all participants.

Nguyen M.T., Nguyen T.T., Nguyen D.B., Nguyen T.M., Nguyen K.N., Ngo V.N., Nguyen V.D., Tran N.A., Lian M., Tan A.S., Chong S.S, & Dang T.T. (2023). Robust preimplantation genetic testing of the common F8 Inv22 pathogenic variant of severe hemophilia A using a highly polymorphic multi-marker panel encompassing the paracentric inversion. Thrombosis Journal, 21, 108.

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