One of the two stimuli comprised the picture taken of the participant wearing standardized gray underwear in their size, under standardized lighting conditions using a digital camera (Canon EOS 1200D with stigma 17–50 mm). The other stimulus was generated using the Rendering Software DAZ Studio 4.9 Pro. The 3D model Victoria 6.0 HD functioned as the basic figure and was rendered to represent an obese female body (see also Voges et al., 2017 ) wearing gray underwear comparable to the real one of the participants. Both stimuli were depicted in frontal view from the neck down, as the head would have drawn too much attention due to its relevance for social information (Hewig et al., 2008 ) and to enable comparison to previous studies which depicted stimuli in the same fashion (Bauer et al., 2017 ; Horndasch et al., 2012 ). Pictures were aligned in terms of body position, with the legs spread hip‐wide and arms spread to the side at a 45° angle. Figure 1 illustrates the obese stimulus, depicting the areas of interest (AOIs) that corresponded to the body parts which participants were asked to rate in terms of attractiveness (see instruments).
Eos 1200d
Manufactured by Canon
Sourced in Japan
The EOS 1200D is a digital single-lens reflex (DSLR) camera manufactured by Canon. It features a 18.0 megapixel CMOS sensor, DIGIC 4 image processor, and a 3-inch LCD screen. The camera supports Full HD 1080p video recording.
Automatically generated - may contain errors
Lab products found in correlation
Compound microscope, by Zeiss (2 mentions)
Cfi plan 10 0.25na 10 5mm wd objective, by Canon (2 mentions)
Ef 70 200mm f 4l usm zoom lens, by Canon (2 mentions)
Stackshot macro rail, by Helicon (2 mentions)
Focus pro 7, by Helicon (2 mentions)
Bx51 compound microscope, by Canon (1 mentions)
Eos utility software, by Canon (1 mentions)
Discovery v8, by Zeiss (1 mentions)
Fv1200, by Olympus (1 mentions)
Fv1000, by Olympus (1 mentions)
Eos 600d, by Canon (1 mentions)
17 protocols using eos 1200d
1
Standardized Stimuli for Body Image Evaluation
2
Detailed Morphological Documentation of Insects
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Legs and details of the male and female genitalia were drawn by means of an Abbe’s drawing apparatus on a compound microscope (Zeiss Jenaval) at magnification 130–500×. Wings were photographed on an Olympus BX51 compound microscope with an attached digital camera (Canon EOS 1200D). Whole adult (dry-mounted) specimens and heads were photographed by means of a Canon EOS 5D Mark III digital camera with a Nikon CFI Plan 10×/0.25NA 10.5mm WD objective attached to a Canon EF 70–200mm f/4L USM zoom lens. The specimen photographed by means of the latter equipment was repositioned upwards between each exposure using a Cognisys StackShot Macro Rail and the final photograph was compiled from multiple layers (~ 40) using Helicon Focus Pro 7.0.2. The final images were edited in Adobe Photoshop CS6. All morphological illustrations were prepared by the first author.
3
Simultaneous Imaging of Strawberry Plant
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An imaging rig was constructed (80 × 70 × 133 cm) to allow simultaneous root and shoot imaging. Cameras were fixed 1 m from the rhizotron surface and 65 cm above the plant canopy. Images were taken with an 18-megapixel full-frame digital single-lens reflex camera (Canon; EOS 1200D) equipped with an 18–55 mm lens (Canon EFS). Illumination was provided by LED-panels with constant illumination. Two cameras were controlled remotely by one laptop with EOS Utility software (Canon, USA Inc., Lake Success, NY) to trigger simultaneous image capture. The minimum detectable size of the colour 24-bit RGB image was ~ 0.1 mm pixel− 1. The resolution of images (230 μm per pixel) could distinguish fine scale strawberry roots. Root and shoot images were taken simultaneously over 6 time points between 7 and 21 days after plant establishment.
4
Microscopic Imaging and Cell Quantification
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Colorimetric images were taken on Zeiss Discovery V8 (Carl Zeiss) microscope with a Canon EOS 600D or Canon EOS 1200D camera. Fluorescent images were taken on either Inverted Olympus FV1000 or FV1200 Confocal microscope. Cells were counted via Adobe Photoshop CS6 or FIJI software and the count was normalized to image area in mm2.
5
Automated Time-Lapse Imaging of Bacterial Growth
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Overnight cultures or washed and resuspended biofilms were plated onto blood agar plates (Columbia + 5% sheep blood, Biomerieux) and placed in a 37 °C incubator. Images were taken by Canon EOS 1200D reflex cameras every 10 min for 48 h. Cameras were triggered by Arduino Uno board and optocouplers. Colonies’ growth curves and radial growth were obtained by analyzing the images with an in-house software. The following number of colonies were analyzed for stationary phase (1A: 75, 14G: 44, 16L: 66) and for biofilms (1A: 71, 14G: 65, 16L: 114).
6
Macroscopic Analysis of Lymphoid Organs
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As part of the macroscopic study, the colour and weight of the lymphoid organs were recorded. The colour of the organs was visually investigated. The weight was measured in gram using an FGH Series high precision balance (AND Company Limited, Korea). The relative weights of the lymphoid organs were calculated using the following formula: (organ weight/body weight) × 100. Required photographs were then captured by using a digital camera (Canon EOS 1200D, Japan) for a better illustration of the macroscopic attributes of lymphoid organs.
7
Photographic Capture of Facial Features
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The participants were photographed by one author (JC), who used a background template (Supplementary Figure S1 ) for guidance and reference of the anatomical parameters (trichion, glabella, mentonian [horizontal], and bipupillary lines [vertical]). A Canon EOS 1200D® digital camera was used with a 105 mm Macro lens and Yongnuo YN-14EX flash. The operator kept the same reference in the ground to take the pictures (1.5 m away from the wall for extraoral photos and 30 cm away for the intraoral photo). All of the photographs were taken in the same place. For intraoral photographs, manual mode was selected (f = 29; ISO = 200; shutter speed = 1/125), and for extraoral pictures, TV mode (ISO = 200; shutter speed = 1/125) was applied. Instructions were given to the participants to take two extraoral frontal photographs: (i) to have the lips relaxed and (ii) afterward, smiling. Intraoral photography was performed using similar retractors and conditions. The picture collection resulted in three pictures per participant (two frontal extraoral photos [resting and smiling] and one frontal intraoral), which were analyzed by another professional (PCL).
8
Wound Healing Efficacy of hEGF Delivery Systems
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Mice were anesthetized with ketamine 40 mg/Kg BW intramuscularly, then placed on a surgical tub in the prone position. The hair around the middle back of the mice was shaved, then cleaned with alcohol-soaked cotton. The wound was made in a circular form with a diameter of 4 mm using a punch biopsy. The test animals were then grouped and marked as follows:
Group I: negative control (without any treatment);
Group II: given the water-soluble chitosan base solution;
Group III: given hEGF KIT solution (dose 75 µg);
Group IV: given recombinant hEGF solution (dose 75 µg);
Group V: given hEGF–liposome suspension (dose 75 µg);
Group VI: given FFSWSC containing hEGF-liposomes (dose 75 µg).
Groups II–VI were given the treatment at intervals of 2 days. Administration of the preparation at intervals of 2 days aimed to observe the effect of controlled release of the chitosan film matrix on the effectiveness of hEGF. The wound area was observed digitally using a camera (Canon EOS 1200D), and the wound area was calculated by ImageJ software until the wound was completely closed.
Group I: negative control (without any treatment);
Group II: given the water-soluble chitosan base solution;
Group III: given hEGF KIT solution (dose 75 µg);
Group IV: given recombinant hEGF solution (dose 75 µg);
Group V: given hEGF–liposome suspension (dose 75 µg);
Group VI: given FFSWSC containing hEGF-liposomes (dose 75 µg).
Groups II–VI were given the treatment at intervals of 2 days. Administration of the preparation at intervals of 2 days aimed to observe the effect of controlled release of the chitosan film matrix on the effectiveness of hEGF. The wound area was observed digitally using a camera (Canon EOS 1200D), and the wound area was calculated by ImageJ software until the wound was completely closed.
9
Basidiocarp Morphology and Microscopy
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Fresh basidiomata were collected from Darjeeling Hills of West Bengal, India during the month of July 2019. Field photographs of the fresh basidiomata were taken at the field with Canon EOS 1200D (Canon, India) camera. For colour notations, Kornerup & Wanscher (1978) was followed. Collected basidiocarps were dried with a field drier at 50-60 °C.
For microscopic observations, free-hand sections were prepared from the dried basidiomata and 5% KOH solution was used to revive those hand-made sections. After staining with Congo red, and Melzer's reagents, sections were observed with Dewinter 'crown' trinocular microscope (Dewinter Optical Inc., New Delhi). Spores were measured with atleast 20 measurements from each of the collected three basidiocarps. In spore statistics, values in parentheses represent minimum or maximum measured values; X m denotes the mean of the spore length by its width (± standard deviation); Q represents range variation of the quotient of basidiospore length/ width ratio in any one basidiospore; Q m , the mean of Q-values (± standard deviation); and n, the total number of spores measured. For future reference, voucher specimens were deposited in the Calcutta University Herbarium (CUH).
For microscopic observations, free-hand sections were prepared from the dried basidiomata and 5% KOH solution was used to revive those hand-made sections. After staining with Congo red, and Melzer's reagents, sections were observed with Dewinter 'crown' trinocular microscope (Dewinter Optical Inc., New Delhi). Spores were measured with atleast 20 measurements from each of the collected three basidiocarps. In spore statistics, values in parentheses represent minimum or maximum measured values; X m denotes the mean of the spore length by its width (± standard deviation); Q represents range variation of the quotient of basidiospore length/ width ratio in any one basidiospore; Q m , the mean of Q-values (± standard deviation); and n, the total number of spores measured. For future reference, voucher specimens were deposited in the Calcutta University Herbarium (CUH).
10
In-situ ROS Detection in Leaves
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In-situ detection of ROS was conducted in the youngest fully expanded leaf as described (Wu et al., 2017) . ROS formation was visualized as brown precipitation, documented by a camera (EOS 1200D, Canon, Tokyo, Japan).
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