All qPCRs were performed on an Applied Biosystems 7300 real-time PCR system. A 25 µl reaction contained 12.5 µl of FastStart SYBR Green Master Mix with Rox (Roche, 04913914001), 8 µl DNase/RNase-free water, 0.75 µl of forward and reverse (10 µM) primers (
Faststart sybr green master mix with rox
The FastStart SYBR Green Master Mix with Rox is a ready-to-use solution for quantitative real-time PCR (qPCR) experiments. It contains a FastStart DNA polymerase, SYBR Green I dye, and a ROX passive reference dye.
Lab products found in correlation
2 protocols using faststart sybr green master mix with rox
Quantitative PCR of Salmonella Typhimurium
All qPCRs were performed on an Applied Biosystems 7300 real-time PCR system. A 25 µl reaction contained 12.5 µl of FastStart SYBR Green Master Mix with Rox (Roche, 04913914001), 8 µl DNase/RNase-free water, 0.75 µl of forward and reverse (10 µM) primers (
Quantifying Gene Expression in Macrophages
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!