Sensolyte adhp hydrogen peroxide assay kit

Manufactured by AnaSpec

Sourced in United States

The SensoLyte ADHP Hydrogen Peroxide Assay kit is a fluorometric assay for the detection and quantification of hydrogen peroxide. The kit utilizes the non-fluorescent ADHP (10-acetyl-3,7-dihydroxyphenoxazine) probe, which becomes highly fluorescent upon reaction with hydrogen peroxide.

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Lab products found in correlation

Tumor necrosis factor (tnf), by Merck Group (1 mentions) Gst rhotekin beads, by Cytoskeleton (1 mentions) Anti gfp antibody, by Thermo Fisher Scientific (1 mentions) Anti rhoa antibody, by Cytoskeleton (1 mentions) Anti phospho src y416 antibody, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Amaxa nucleofector kit, by Lonza (1 mentions) Protease inhibitor, by Thermo Fisher Scientific (1 mentions)

2 protocols using sensolyte adhp hydrogen peroxide assay kit

Myristoylated G13BP Modulates VE-Cadherin

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Myristoylated G13BP and scrambled peptide were synthesized and purified at the Research Resource Center at University of Illinois, Chicago. Anti-Gα13 (sc-410), anti-Src (sc-18, sc-5266), and anti–VE-cadherin (sc-9989 and sc-6458) antibodies, as well as human VE-cadherin siRNA were purchased from Santa Cruz Biotechnology; mouse monoclonal anti-Gα13antibody (26004) was from Neweast Biosciences; anti–phospho-Src Y416 antibody was obtained from Cell Signaling Technology; anti–phospho-VE-cadherin Y658 antibody, Src kinase assay kit, and anti–v-Src antibody were obtained from Millipore; anti–β-actin and anti-Flag (M2) antibodies, TNF, GDT and GTPγS, NAC, and DPI were purchased from Sigma-Aldrich; Lipofectamine 2000, anti-GFP antibody, and Lipofectamine 2000 were purchased from Invitrogen; HMVEC-L and Amaxa Nucleofector kit (VPB-1002) were obtained from Lonza; and HisPur Ni-NTA Spin Purification kit was obtained from Pierce. SensoLyte ADHP Hydrogen Peroxide Assay kit was from purchased AnaSpec. Anti-RhoA antibody and GST-Rhotekin beads were purchased from Cytoskeleton Inc.

Gong H., Gao X., Feng S., Siddiqui M.R., Garcia A., Bonini M.G., Komarova Y., Vogel S.M., Mehta D, & Malik A.B. (2014). Evidence of a common mechanism of disassembly of adherens junctions through Gα13 targeting of VE-cadherin. The Journal of Experimental Medicine, 211(3), 579-591.

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Measuring Kidney Hydrogen Peroxide

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To examine hydrogen peroxide levels in the kidney, whole kidney tissue was homogenized in a tissue protein extraction reagent with a protease inhibitor (Thermo Fisher Scientific). Hydrogen peroxide levels were measured using the SensoLyte ADHP Hydrogen Peroxide Assay Kit (AnaSpec, Fremont, CA, USA).

Mizokami T., Shimada M, & Suzuki K. (2024). Neutrophil depletion attenuates acute renal injury after exhaustive exercise in mice. Experimental Physiology, 109(4), 588-599.

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