1640 medium

Manufactured by Wisent

Sourced in China, United States

The 1640 medium is a laboratory culture medium used for the in vitro cultivation of cells. It provides the necessary nutrients and growth factors to support the growth and maintenance of various cell types. The composition and formulation of the 1640 medium are designed to support the specific requirements of cell culture applications.

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Lab products found in correlation

Jetprime transfection reagent, by Polyplus Transfection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Wisent (2 mentions) Dmem medium, by Wisent (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Wisent (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by ExCell Bio (1 mentions) Bovine serum albumin (bsa), by Wisent (1 mentions) Protein assay kit, by Bio-Rad (1 mentions) Polyethylene glycol, by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Dulbecco s modified eagle medium dmem, by Wisent (1 mentions) J774 macrophage like cells, by American Type Culture Collection (1 mentions)

8 protocols using 1640 medium

Kupffer Cell Modulation in NASH

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Kupffer cells (NASH-associated mouse Kupffer cell lines, BeNa Culture Collection, China, BNCC340733) were cultured in Roswell Park Memorial Institute 1640 medium (Cat No. 350-000-CL, Wisent, Nanjing, China) containing 10% (v/v) fetal bovine serum at 37 °C in an atmosphere of 5% CO₂. At 70% confluent growth Kupffer cells were pretreated with 50 μM DHA/AA for 4 h and then treated with 100 ng/mL LPS for 6 or 12 h.
For GPR120 knockdown, specific small interfering RNAs (GPR120 siRNA1, GPR120 siRNA2, and GPR120 siRNA3; GenePharma, Shanghai, China) were transfected into Kupffer cells using jetPRIME^® transfection reagent (Polyplus Transfection, Beijing, China). The scramble siRNA was used as a negative control (NC siRNA). The sequences are listed in Supplementary Table S1.

Fan G., Li Y., Chen J., Zong Y, & Yang X. (2021). DHA/AA alleviates LPS-induced Kupffer cells pyroptosis via GPR120 interaction with NLRP3 to inhibit inflammasome complexes assembly. Cell Death & Disease, 12(1), 73.

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Cell Culture Protocol for Cancer Research

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NCI‐H82, G‐401, MDA‐MB‐453, SW48, 786‐O, CFPAC‐1 cells, and HEK293T were sourced from ATCC, WSU‐DLCL2 cell was from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) and SF126 cell was from JCRB Cell Bank. NCI‐H82, G‐401, WSU‐DLCL2, and 786‐O were cultured in 1640 medium (WISENT, 350‐000‐CL) with 10% FBS, 1% Pen/Strep. SW48, SF‐126, and HEK293T were cultured in DMEM medium (WISENT, 319‐005‐CL) with 10% FBS, 1% Pen/Strep. CFPAC‐1 were cultured in IMDM medium (MINGZHOUBIO, MPM150510) with 10% FBS, 1% Pen/Strep. MDA‐MB‐453 was cultured in L‐15 medium (WISENT, 323‐050‐CL) with 10% FBS, 1% Pen/Strep. MDA‐MB‐453 was maintained in 100% air at 37 °C and other cells were in 5% CO₂ at 37 °C.

Wu C., Liu Y., Liu W., Zou T., Lu S., Zhu C., He L., Chen J., Fang L., Zou L., Wang P., Fan L., Wang H., You H., Chen J., Fang J., Jiang C, & Shi Y. (2022). NNMT‐DNMT1 Axis is Essential for Maintaining Cancer Cell Sensitivity to Oxidative Phosphorylation Inhibition. Advanced Science, 10(1), 2202642.

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Xenograft Model of Human Gastric Cancer

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The MKN-45 human gastric cancer cell line was obtained from the Shanghai Institute of Cell Biology. MKN-45 cells were cultured in Roswell Park Memorial Institute 1640 medium (Wisent, Nanjing, People’s Republic of China) with 10% fetal bovine serum (Gibco, Carlsbad, CA, USA). Cultures were maintained at 37°C with 5% CO₂ in a humidified incubator. Male Balb/c nude athymic mice at 4–6 weeks old were supplied by the Department of Experimental Animals, Yangzhou University. All animal experiments were carried out in accordance with the Guide for the Care and Use of Laboratory Animals approved by Nanjing Drum Tower Hospital Laboratory Animals Welfare & Ethical Committee, People’s Republic of China.

Zhang L., Li R., Chen H., Wei J., Qian H., Su S., Shao J., Wang L., Qian X, & Liu B. (2017). Human cytotoxic T-lymphocyte membrane-camouflaged nanoparticles combined with low-dose irradiation: a new approach to enhance drug targeting in gastric cancer. International Journal of Nanomedicine, 12, 2129-2142.

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ACC2 Knockdown in FaDu and NP69 Cells

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The FaDu cells and NP69 cells were purchased from American Type Culture Collection. The FaDu cells or NP69 cells were cultured in minimum essential medium Eagle’s (Mod.) or 1,640 medium (Wisent, USA) supplemented with 10% fetal bovine serum (Gibco, USA) and antibiotics (100 KU/L penicillin and 100 mg/L streptomycin) in an incubator at 37 °C with 5% CO₂. The FaDu and NP69 cells were transiently transfected with ACC2 siRNA (sense, CCUGCCUACUUUCUUCUAUTT; antisense, AUAGAAGAAAGUAGGCAGGTT) (GenePharma, Shanghai, China) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions and were cultured for another 48 h before being used in experiments.

Li K., Zhang C., Chen L., Wang P., Fang Y., Zhu J., Chen S., Du J., Shen B., Wu K, & Liu Y. (2019). The role of acetyl-coA carboxylase2 in head and neck squamous cell carcinoma. PeerJ, 7, e7037.

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Culturing Clear Cell Renal Cancer Lines

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Human clear cell renal cell carcinoma cell lines 786-O and 769-P, and human kidney cell line 293T were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). 786-O and 769-P cells were cultured in 1640 medium (Wisent, Nanjing, China). 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) medium (Wisent, Nanjing, China). The culture medium was supplemented with 1% penicillin/streptomycin (Wisent, Nanjing, China) and 10% fetal bovine serum (FBS) (Wisent, Nanjing, China). Cells were cultured in a 37 °C humidified incubator containing 5% CO₂.

Xu J., Zhu S., Xu L., Liu X., Ding W., Wang Q., Chen Y, & Deng H. (2020). CA9 Silencing Promotes Mitochondrial Biogenesis, Increases Putrescine Toxicity and Decreases Cell Motility to Suppress ccRCC Progression. International Journal of Molecular Sciences, 21(16), 5939.

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KRAS G12C Lung Adenocarcinoma Cell Lines

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Lung adenocarcinoma cell lines harbor KRAS G12C mutations were obtained from American Type Culture Collection. NCI-H23 (H23) and NCI-H358 (H358) cells were cultured in Roswell Park Memorial Institute 1640 medium (WISENT Inc., Nanjing, China) and supplemented with 10% fetal bovine serum (Excell Bio Inc., Taicang, China) and 50 µg/ml penicillin–streptomycin (Invitrogen). All cells used in this study were maintained in a 5% CO₂ cell culture incubator at 37 °C. AMG-510 and Cisplatin (Targetmol Co. Ltd., Shanghai, China) were dissolved in dimethyl sulfoxide and H2O, respectively, then stored at -80 °C until use. Untreated cell lines were subdued until cell viability assays for determining the concentration inhibiting 50% of cell viability (IC50).

Wu L.L., Jiang W.M., Liu Z.Y., Zhang Y.Y., Qian J.Y., Liu Y., Huang Y.Y., Li K., Li Z.X., Ma G.W, & Xie D. (2023). AMG-510 and cisplatin combination increases antitumor effect in lung adenocarcinoma with mutation of KRAS G12C: a preclinical and translational research. Discover. Oncology, 14, 91.

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Kupffer Cell LPS Response via Mtfmt Knockdown

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Kupffer cells (BeNa Culture Collection, Kunshan, China, BNCC340733) were cultured at 37 °C in a 5% CO₂ atmosphere in 1640 medium (Wisent, cat. no.: 350-000-CL, Nanjing, China) containing 10% (v/v) fetal bovine serum. During 70% confluent growth, Kupffer cells were treated for 6 or 12 h with 100 ng/mL LPS [48 (link)]. Using jetPRIME^® transfection reagent (Polyplus Transfection, Beijing, China), specific small interfering RNAs (Mtfmt siRNA1, Mtfmt siRNA2, and Mtfmt siRNA3; GenePharma, Shanghai, China) were transfected into Kupffer cells to knock down Mtfmt. The scramble siRNA served as a negative control (NC siRNA). The sequences are listed in Supplementary Table S2.

Sun X., Liu S., Cai J., Yang M., Li C., Tan M, & He B. (2023). Mitochondrial Methionyl-tRNA Formyltransferase Deficiency Alleviates Metaflammation by Modulating Mitochondrial Activity in Mice. International Journal of Molecular Sciences, 24(6), 5999.

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Macrophage-Mediated Lipoprotein Analysis

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Sodium dodecyl sulfate (SDS), ethylenediaminetetraacetic acid (EDTA), and polyethylene glycol (PEG) were purchased from Sigma-Aldrich (Oakville, ON, Canada). Bovine serum albumin (BSA), fetal bovine serum (FBS), Dulbecco’s modified eagle medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640 medium, and penicillin/streptomycin were purchased from Wisent (St-Bruno, QC, Canada). J774 macrophage-like cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Bio-Rad Protein Assay kits were obtained from Bio-Rad Laboratories (Mississauga, ON, Canada). Lipoprint^® HDL kits were obtained from Quantimetrix (Redondo Beach, CA, USA). Extra virgin olive oil (EVOO) was obtained from Atlas Olive Oils sarl (Casablanca, Morocco).

Otrante A., Trigui A., Walha R., Berrougui H., Fulop T, & Khalil A. (2021). Extra Virgin Olive Oil Prevents the Age-Related Shifts of the Distribution of HDL Subclasses and Improves Their Functionality. Nutrients, 13(7), 2235.

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