Anti atm

Manufactured by GeneTex

The Anti-ATM product is a laboratory equipment designed to detect and measure the levels of ATM (Ataxia Telangiectasia Mutated) protein in biological samples. ATM is a key regulator of cellular response to DNA damage and is involved in various cellular processes. The Anti-ATM product provides a reliable and accurate method for researchers to study the expression and activity of ATM in their investigations.

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Lab products found in correlation

3 protocols using anti atm

Cellular Protein Expression Analysis by Western Blotting

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Cellular proteins were extracted using kits from Active Motif (Carlsbad, CA). Expression levels were examined following standard Western blotting protocols as described (4 (link)). Primary antibodies used: anti-G6PD (#12263S, Cell signaling Technology), anti-ATM (#NB100-306, GeneTex), anti-phospho-ATM (#GTX70103, Novus Biologicals). HRP-conjugated anti-GAPDH (#3683, Cell signaling technology) or anti-β-actin (#5125, Cell signaling technology) served as internal controls.

Yang Z., Shen Y., Oishi H., Matteson E.L., Tian L., Goronzy J.J, & Weyand C.M. (2016). Restoring oxidant signaling suppresses pro-arthritogenic T-cell effector functions in rheumatoid arthritis. Science translational medicine, 8(331), 331ra38.

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Western Blot Analysis of Cell Signaling

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Samples were treated with lysis buffer [Tris–HCl 50mM pH7.4, 10% NP-40, 0.25% NaDesoxycholate, EDTA 1mM, NaCl 150mM, PMSF 1mM, protease inhibitor cocktail (Roche)] and loaded onto pre-cast 4–12% gradient acrylamide gels (NuPAGE, Invitrogen). After electro-blotting, filters were incubated with anti-AKTIP (Sigma), anti-TRF2 (Novus Biologicals), anti-actin-HRP conjugated (Santa Cruz), anti-cyclin A (Santa Cruz), anti-cyclin B (Santa Cruz), anti-cyclin E (Upstate Biotechnology), anti-p53-pSer15 (Cell Signaling Technology), anti-p53 (DakoCytomation), anti-ATM-pS1981 (Rockland Immunochemicals), anti-ATM (Genetex), anti-ChK1-PSer345 (Cell Signaling Technology), anti-AKT (Cell Signaling Technology), anti-PCNA (Santa Cruz), anti-RPA70 (Santa Cruz), or anti-TRF1 (Santa Cruz). Filters were then incubated with appropriate HRP-conjugated secondary antibodies (Santa Cruz), which were detected using the enhanced chemiluminescence system (ECL plus, Amersham). Signals were quantified with Image J software.

Burla R., Carcuro M., Raffa G.D., Galati A., Raimondo D., Rizzo A., La Torre M., Micheli E., Ciapponi L., Cenci G., Cundari E., Musio A., Biroccio A., Cacchione S., Gatti M, & Saggio I. (2015). AKTIP/Ft1, a New Shelterin-Interacting Factor Required for Telomere Maintenance. PLoS Genetics, 11(6), e1005167.

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Comprehensive Antibody Characterization for Research

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Detailed information of antibodies used in this study: anti‐Flag (Sigma, Rabbit, F7425), anti‐TopBP1 (Santa Cruz, Mouse, sc‐271043; BETHYL, Rabbit, A300‐111A), anti‐γH2AX (Novus, Rabbit, NB100‐384; CST, Rabbit, 9718), anti‐SCP3 (Santa Cruz, Mouse, sc‐74569; Novus, Rabbit, NB300‐232), anti‐rRNA (Novus, Mouse, NB100‐662), anti‐RPL7A (ABclonal, Rabbit, A13713), anti‐RPS3 (ABclonal, Rabbit, A2533), anti‐FBL (CST, Rabbit, 2639S; Santa cruz, Mouse, sc‐166001), IgG (Rabbit, CST, 2729), IgG (Mouse, CST, 3420), anti‐ATR (BETHYL, Rabbit, A300‐137A), anti‐Chk1 (Novus, Rabbit, NB100‐464), anti‐pATR (T1989) (GeneTex, Rabbit, GTX128145), anti‐pChk1 (Ser345) (CST, Rabbit, 2348), anti‐GAPDH (CST, Mouse, 97 166), anti‐pH3 (Ser10) (CST, Rabbit, 9701), anti‐Puromycin (Millipore, Mouse, MABE343), anti‐GAPDH (HUABIO, Rabbit, ET1601‐4), anti‐Histone H3 (CST, Rabbit, 4499S), anti‐ATM (GeneTex, Mouse, GTX70107), anti‐pATM (S1981) (ROCKLAND, Mouse, 200‐301‐400), anti‐pRPA (Novus, Rabbit, NBP1‐23017), anti‐Rad9 (Santa Cruz, Mouse, sc‐74464), anti‐RAD51 (abcam, Rabbit, ab63801).

Xin D., Gai X., Ma Y., Li Z., Li Q, & Yu X. (2023). Pre‐rRNA Facilitates TopBP1‐Mediated DNA Double‐Strand Break Response. Advanced Science, 10(28), 2206931.

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