Log In Sign up
The largest database of trusted experimental protocols

Pp242

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

PP242 is a small-molecule inhibitor that selectively targets the mTOR kinase. It is commonly used in biochemical and cell-based research applications.

Automatically generated - may contain errors

Lab products found in correlation

Akt inhibitor x, by Santa Cruz Biotechnology (4 mentions) Celltiter 96 aqueous one solution cell proliferation assay kit, by Promega (3 mentions) Anti cd19 magnetic fluorobeads b, by Thermo Fisher Scientific (2 mentions) Cadmium chloride, by Merck Group (1 mentions) 5 and 6 chloromethyl 2 7 dichlorodihydrofluorescein diacetate cm h2dcfda, by MP Biomedicals (1 mentions) Sp600125, by Merck Group (1 mentions) Neurobasal media, by Thermo Fisher Scientific (1 mentions) B27 supplement, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Resveratrol, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) 3 methyladenine, by Merck Group (1 mentions) Recombinant tnf α, by R&D Systems (1 mentions) β actin antibody, by Cell Signaling Technology (1 mentions) Lc3b antibody, by Merck Group (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Ad mcherry gfp lc3b, by Beyotime (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) Ml sa1, by Abcam (1 mentions) Verapamil hydrochloride, by MedChemExpress (1 mentions)

6 protocols using pp242

1

Pharmacological Inhibition of mTOR Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rapamycin (LC Laboratories, Woburn, MA, USA), PP242 and Akt inhibitor X (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were dissolved in dimethyl sulfoxide (DMSO) to prepare 100 μg/ml Rapamycin, 1 mM PP242 and 10 mM Akt inhibitor X as stock solutions, and stored at −20°C. IGF-1 (PeproTech, Rocky Hill, NJ, USA) was rehydrated in 0.1 M acetic acid to prepare a 10 μg/ml stock solution and stored at −80°C. Bovine collagen type IV (CN IV) was purchased from Rockland Immunochemicals (Gilbertsville, PA, USA). 7-methyl GTP Sepharose 4B was from GE Healthcare Life Sciences (Piscataway, NJ, USA). CellTiter 96® AQueous One Solution Cell Proliferation Assay Kit was from Promega (Madison, WI, USA). Enhanced chemiluminescence solution was from Pierce (Rockford, IL, USA). Antibodies included those against S6K1, Akt, S6, eIF4E, GSK3β, HA (Santa Cruz Biotechnology, Santa Cruz, CA, USA), mTOR, phospho-S6K1 (Thr389), phospho-Akt (Ser473), phospho-Akt (Thr308), phospho-S6 (Ser/235/236), 4E-BP1, phospho-4E-BP1 (Thr70) (Cell Signaling, Beverly, MA, USA), raptor, rictor (Bethyl Laboratories, Montgomery, TX, USA), phospho-GSK3β (Ser9) (Epitomics, Burlingame, CA, USA), β-tubulin, FLAG (Sigma, St. Louis, MO, USA), goat anti-rabbit IgG-horseradish peroxidase (HRP), goat anti-mouse IgG-HRP, and rabbit anti-goat IgG-HRP (Pierce). All other chemicals were purchased from Sigma.
Chen L., Xu B., Liu L., Liu C., Luo Y., Chen X., Barzegar M., Chung J, & Huang S. (2015). Both mTORC1 and mTORC2 are involved in the regulation of cell adhesion. Oncotarget, 6(9), 7136-7150.
+ Open protocol
+ Expand
2

Antioxidant and Cell Signaling Pathway Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Resveratrol, cadmium chloride, poly-d-lysine (PDL), U0126, SP600125 and protease inhibitor cocktail were purchased from Sigma (St Louis, MO, USA). Rapamycin was bought from ALEXIS (San Diego, CA, USA), whereas 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) was from MP Biomedicals (Solon, OH, USA). PP242 and Akt inhibitor X were provided by Santa Cruz Biotechnology (Santa Cruz Biotechnology, Dallas, TX, USA). Dulbecco’s modified Eagle medium (DMEM), 0.05% Trypsin-EDTA, NEURO-BASAL™ Media, and B27 Supplement were purchased from Invitrogen (Grand Island, NY, USA). Horse serum and fetal bovine serum (FBS) were supplied by Hyclone (Logan, UT, USA). Enhanced chemiluminescence solution was from Sciben Biotech Company (Nanjing, China). Other chemicals used in this work were obtained from local commercial sources and were of analytical grade.
Liu C., Zhang R., Yang L., Ji T., Zhu C., Liu B., Zhang H., Xu C., Zhang N., Huang S, & Chen L. (2022). Neuroprotection of resveratrol against cadmium-poisoning acts through dual inhibition of mTORC1/2 signaling. Neuropharmacology, 219, 109236.
+ Open protocol
+ Expand
3

Flow Cytometry Assay for Lymphocyte Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-CD19 magnetic fluorobeads-B was purchased from One Lambda (Canoga Park, CA, USA). PP242 (mTORC1/2 kinase inhibitor) and Akt inhibitor X were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rapamycin (mTOR inhibitor) was from ALEXIS (San Diego, CA, USA). Refolded human soluble BAFF (hsBAFF) was a recombinant form of the extracellular domain of the BAFF produced in Escherichia coli from this group (Cao et al., 2005 (link)). RPMI 1640 was from Gibco (Rockville, MD, USA). Fetal bovine serum (FBS) was supplied by Hyclone (Logan, UT, USA). CellTiter 96® AQueous One Solution Cell Proliferation Assay kit was from Promega (Madison, WI, USA). Other chemicals were purchased from local commercial sources and were of analytical grade.
Zeng Q., Qin S., Zhang H., Liu B., Qin J., Wang X., Zhang R., Liu C., Dong X., Zhang S., Huang S, & Chen L. (2017). Rapamycin attenuates BAFF-extended proliferation and survival via disruption of mTORC1/2 signaling in normal and neoplastic B-lymphoid cells. Journal of cellular physiology, 233(1), 516-529.
+ Open protocol
+ Expand
4

Regulation of Intestinal Barrier Integrity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Caco-2 cells and IEC-6 cells were obtained from the Cell Bank of the Chinese Academy of Sciences (analog no. TCHu146, Shanghai, China) and Shanghai Fuxiang Biotechnology (catalog no. CRL-1592, Shanghai, China) respectively. Cells were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Cells were maintained in a 5% CO2/95% air atmosphere at 37 °C. Recombinant TNF-α was obtained from R&D Systems (catalog no. 510-RT-010, Minneapolis, MN, USA). Bafilomycin A1 (catalog no. 189490) and 3-methyladenine (catalog no. 19-148) were purchased from EMD Millipore (Temecula, CA, USA). PP242 was purchased from Santa Cruz Biotechnology (catalog no. sc-301606, Santa Cruz, CA, USA). Claudin-2 antibody (catalog no. 51-6100, Invitrogen, Carlsbad, CA, USA), LC3B antibody (catalog no. L7543, Sigma, St. Louis, MO, USA), P62 antibody (catalog no. sc-25575, Sancta Cruz Biotechnology, Santa Cruz, CA, USA), and β-actin antibody (catalog no. 4970, Cell Signaling Technology, Danvers, MA, USA) were purchased from relevant manufacturers. Ad-mCherry-GFP-LC3B was purchased from Beyotime Biotechnology (catalog no. C3011, Shanghai, China).
Zhang C., Yan J., Xiao Y., Shen Y., Wang J., Ge W, & Chen Y. (2017). Inhibition of Autophagic Degradation Process Contributes to Claudin-2 Expression Increase and Epithelial Tight Junction Dysfunction in TNF-α Treated Cell Monolayers. International Journal of Molecular Sciences, 18(1), 157.
+ Open protocol
+ Expand
5

Anti-CD19 Magnetic Fluorobeads-B Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-CD19 magnetic fluorobeads-B was purchased from One Lambda (Canoga Park, CA, USA). Akt inhibitor X and PP242 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Refolded human soluble BAFF (hsBAFF) was a recombinant form of the extracellular domain of the BAFF produced in Escherichia coli from this group [35 (link)]. RPMI 1640 medium and fetal bovine serum (FBS) were supplied by Gibco (Rockville, MD, USA). CellTiter 96®AQueous One Solution Cell Proliferation Assay kit was from Promega (Madison, WI, USA). Enhanced chemiluminescence solution was from Sciben Biotech Company (Nanjing, China). 1,2-bis(oAminophenoxy) ethane-N,N,N’,N’-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM) and 2-aminoethoxydiphenyl borane (2-APB) were purchased from Calbiochem (San Diego, CA, USA), whereas ethylene glycol tetra-acetic acid (EGTA) was purchased from Sigma (St. Louis, MO, USA). ML-SA1 was supplied by Abcam Technology (Cambridge, UK). Verapamil hydrochloride was from MedChemExpress (Monmouth Junction, NJ, USA). Rapamycin and KN93 were from ALEXIS (San Diego, CA, USA), while U0126 was from Sigma. Other chemicals were purchased from local commercial sources and were of analytical grade.
Zeng Q., Zhou Z., Qin S., Yao Y., Qin J., Zhang H., Zhang R., Xu C., Zhang S., Huang S, & Chen L. (2020). Rapamycin inhibits B-cell activating factor (BAFF)-stimulated cell proliferation and survival by suppressing Ca2+-CaMKII-dependent PTEN/Akt-Erk1/2 signaling pathway in normal and neoplastic B-lymphoid cells. Cell calcium, 87, 102171.
+ Open protocol
+ Expand
6

Amino Acid Starvation and Protein Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
For amino acid starvation, cells were washed two times with starvation medium (140 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM glucose, 1% BSA, and 20 mM HEPES (pH: 7.4), and incubated with the same starvation medium for 1 h. Pp242 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was dissolved in DMSO in a 1 mM stock solution and applied over 1 h at a 1 μM final concentration. Chloroquine diphosphate salt (Sigma Aldrich, Darmstadt, Germany) was dissolved in PBS in a 10 mM stock and applied over 16 or 24 h at a 50 μM final concentration, or over 2 to 8 h at a 100 50 μM concentration. MG132 (PeptaNova, Sandhausen, Germany) was dissolved in DMSO in a 10 mM stock solution and applied over 6 h at a 10 μM final concentration. Cycloheximide (CHX) (Sigma-Aldrich) was applied over 2, 4, 6, 8, or 16 h at a 100 μg/mL concentration. MLN4924 (Selleckchem, Houston, TX, USA) was dissolved in DMSO in a 5 mM stock solution and was applied over 5 h at a 1 μM concentration.
Renna F.J., Enriqué Steinberg J.H., Gonzalez C.D., Manifava M., Tadic M.S., Orquera T., Vecino C.V., Ropolo A., Guardavaccaro D., Rossi M., Ktistakis N.T, & Vaccaro M.I. (2023). Ubiquitination Is a Novel Post-Translational Modification of VMP1 in Autophagy of Human Tumor Cells. International Journal of Molecular Sciences, 24(16), 12981.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!