Single cell suspensions from nasal mucosa were resuspended in PBS/0.1% bovine serum albumin (BSA) seeded in a 96-well plate at 106 cells/ml, blocked with 5% goat serum and stained with Phycoerythrin conjugated anti-CD107a (eBioscience, San Diego, California, USA) and anti-Siglec-F (BD biosciences) for the detection of total degranulation40 (link) and eosinophils, respectively. For in vitro assessment of BMMC expression of FCεRI, cells were stained with anti-FCεRI (eBioscience, San Diego, California, USA) and cell viability was determined by propidium iodide staining (Sigma Aldrich, Rehovot, Israel). Data were analyzed by a FACSCalibur and CellQuest software (Becton Dickinson, San Jose California, USA).
Anti fcεri
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-FCεRI is a laboratory equipment product designed to detect and measure the expression of the high-affinity IgE receptor, FcεRI, on the surface of cells. It is a useful tool for researchers studying the role of FcεRI in various biological processes, such as allergic responses and immune function.
Automatically generated - may contain errors
Lab products found in correlation
Anti siglecf, by BD (2 mentions)
Phycoerythrin conjugated anti cd107a, by Thermo Fisher Scientific (2 mentions)
Facscalibur, by BD (2 mentions)
Cellquest, by BD (2 mentions)
Thymol, by Merck Group (1 mentions)
Ruthenium red, by Merck Group (1 mentions)
Probenecid, by Merck Group (1 mentions)
Fluo 4 am, by Thermo Fisher Scientific (1 mentions)
Anti dnp ige, by Merck Group (1 mentions)
Dnp hsa, by Merck Group (1 mentions)
Annexin 5, by Thermo Fisher Scientific (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Anti cd117, by BD (1 mentions)
Anti cd16 32, by BD (1 mentions)
2 apb, by Merck Group (1 mentions)
Hc 030031, by Merck Group (1 mentions)
3 protocols using anti fcεri
1
Quantification of Degranulation and Eosinophils in Nasal Mucosa
2
Quantification of Degranulation and Eosinophils in Nasal Mucosa
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single cell suspensions from nasal mucosa were resuspended in PBS/0.1% bovine serum albumin (BSA) seeded in a 96-well plate at 106 cells/ml, blocked with 5% goat serum and stained with Phycoerythrin conjugated anti-CD107a (eBioscience, San Diego, California, USA) and anti-Siglec-F (BD biosciences) for the detection of total degranulation40 (link) and eosinophils, respectively. For in vitro assessment of BMMC expression of FCεRI, cells were stained with anti-FCεRI (eBioscience, San Diego, California, USA) and cell viability was determined by propidium iodide staining (Sigma Aldrich, Rehovot, Israel). Data were analyzed by a FACSCalibur and CellQuest software (Becton Dickinson, San Jose California, USA).
3
Mast Cell Activation Assay
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