Anti viperin

Mouse monoclonal anti-poliovirus 2C and 2B were described in [105 (link)]. Rabbit polyclonal anti-polio 3D antibodies were developed by Chemicon using recombinant 3D protein as immunogen. Rabbit monoclonal anti-CCTα, anti-eIF2α, anti-eIF2α (phospho Ser51), anti-IRF3, anti-IRF3 (phospho Ser396), anti-HSL, anti-ATGL, anti-STAT1, anti-Viperin, anti-ISG15 and anti-HA antibodies used in western blots and immunofluorescence were from Cell Signaling. Mouse monoclonal anti-dsRNA antibodies were from English and Scientific Consulting Kft. Mouse monoclonal anti-GBF1 antibodies were from BD Biosciences; rabbit anti-PI4KIIIβ were from EMD Millipore. Mouse monoclonal antibodies against Iκβ were a kind gift from Dr. John Patton (University of Maryland). Alexa dyes conjugated antibodies were from Molecular Probes (Thermo Fisher); secondary HRP-conjugated antibodies were from Amersham. Mouse monoclonal anti-HA antibody used for co-IP was from Santa Cruz Biotechnology.

Primary antibodies used were mouse anti-HA (901502, Biolegend), rabbit anti-HA (51064-2-AP, Proteintech), anti-FLAG (F7425, Sigma), anti-puromycin (12D10, Sigma), anti-viperin (MaP.VIP) (44 (link)), anti-RIG-I (3743, Cell Signaling Technology), anti-MDA5 (5321, Cell Signaling Technology), anti-TRIM21 (12108-1-AP, Proteintech), anti-ISG15 (200401438, Rockland), anti-STING (13647, Cell Signaling Technology), anti-GRP94 (9G10, Enzo), and anti-GAPDH (10494-1-AP, Proteintech). Anti-SARS-CoV ORF3a was a kind gift from Carolyn Machamer (Johns Hopkins University, Baltimore, MD). All secondary antibodies used for immunofluorescence imaging and immunoblotting were purchased from Invitrogen and Jackson ImmunoResearch, respectively.

The primary antibodies used were rabbit anti-hCMPK2 (peptide sequence: amino acids 48–61 made by GenScript), anti-Flavivirus E protein (MAB10216; Millipore), anti-FLAG (F7425; Sigma), anti-viperin (MaP.VIP) [78 (link)], anti-RIG-I (3743; Cell Signaling Technology), anti-GRP94 (9G10; Enzo), anti-GAPDH (10494-1-AP; Proteintech) and anti-dsRNA J2 (RNT-SCI-10010200; Jena Bioscience). All secondary antibodies used for immunofluorescence imaging and immunoblotting were purchased from Invitrogen and Jackson ImmunoResearch, respectively.