Dopet

Manufactured by Merck Group

Sourced in Germany

DOPET is a laboratory equipment product developed by Merck Group. It is designed for the precise measurement and analysis of dopamine levels. The core function of DOPET is to provide accurate and reliable data on dopamine concentrations in various sample types.

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Lab products found in correlation

Dopac, by Merck Group (2 mentions) Thioflavin t tht, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Hplc grade, by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Tolcapone, by Orion Pharma (1 mentions) Rat pheochromocytoma pc12 cells, by American Type Culture Collection (1 mentions) Human glioblastoma cells, by American Type Culture Collection (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

4 protocols using dopet

FENÒLIA® Olive Oil Capsule Protocol

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Ethylenediaminetetraacetic Acid (EDTA), citric acid, L-ascorbic acid, β-glucuronidase type H2 from Helix pomatia, LC-MS grade formic acid (HCOOH), methanol, HPLC-grade (purity ≥ 97%) DOPET, 3,4-dihydroxyphenylacetic acid (DOPAC), 4-hydroxy-3-methoxyphenethanol (MOPET) and homovanillic acid (HVA) were purchased from Merck KGaA (Darmstadt, Germany).
The pharmaceutical formulation FENÒLIA^® enteric-coated capsules, kindly provided by P&P Farma (Turin, Italy), consists of extra virgin organic olive oil (Olea Europaea L., oleum ex fructibus), gelatin (shell component), coating agent: E1420, anti-caking agents: talc, silicon dioxide, dry olive extract (Olea europaea L., fructus) 15% titrated in DOPET, vitamin E (DL-alpha tocopheryl acetate), stabilizer: glycerol and pigment: E 171, E 141, E 161b.

Di Renzo L., Smeriglio A., Ingegneri M., Gualtieri P, & Trombetta D. (2023). The Pharmaceutical Formulation Plays a Pivotal Role in Hydroxytyrosol Pharmacokinetics. Pharmaceutics, 15(3), 743.

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Preparation of Catechol Solutions

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DOPAC, DOPET, Thioflavin T (ThT), and lyophilized bovine catalase (CAT) were purchased from Merck (Darmstadt, Germany). The catechol powder was dissolved in water solution at 100 mM final concentration and stored at −20°C.⁵⁰ All reagents and chemicals were obtained from Sigma or Fluka (St. Louis, MO, USA) and were of analytical reagent grade.

Fongaro B., Cappelletto E., Sosic A., Spolaore B, & de Polverino de Laureto P. (2022). 3,4‐Dihydroxyphenylethanol and 3,4‐dihydroxyphenylacetic acid affect the aggregation process of E46K variant of α‐synuclein at different extent: Insights into the interplay between protein dynamics and catechol effect. Protein Science : A Publication of the Protein Society, 31(7), e4356.

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Catecholamine Metabolism in Cell Lines

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Rat pheochromocytoma PC12 cells and human glioblastoma cells were from the American Type Culture Collection (ATCC, Manassas, VA; PC12 cells catalog no. CRL-1721, glioblastoma cells catalog no. HTB-15). The cell culture media, F12K and DMEM, were from Gibco Life Technologies (Grand Islands, NY); tolcapone (to block catechol-O-methyltransferase) from Orion Pharma (Espoo, Finland); and dopamine, norepinephrine, DOPAC, DOPET, and DHPG from Sigma. 6F-Dopamine was obtained in solution (about 1.35 mM) from the NIH PET Department after having passed quality control for i.v. administration to humans. DOPAL standard was synthesized in the laboratory of and provided by Dr. Kenneth L. Kirk (NIDDK).

Goldstein D.S., Sullivan P., Cooney A., Jinsmaa Y., Kopin I.J, & Sharabi Y. (2015). Rotenone Decreases Intracellular Aldehyde Dehydrogenase Activity: Implications for the Pathogenesis of Parkinson Disease. Journal of neurochemistry, 133(1), 14-25.

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Reagent Acquisition for Bioassay

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DOPET and LPS were obtained from Sigma-Aldrich, USA. The other reagents used in the study were obtained from Merck, USA.

Zhang L., Wen K., Zhang Z., Ma C, & Zheng N. (2021). 3,4-Dihydroxyphenylethanol ameliorates lipopolysaccharide-induced septic cardiac injury in a murine model. Open Life Sciences, 16(1), 1313-1320.

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