Poly-L-lysine coated slides were placed in a 6 well plate and 150 k cells/well were seeded and incubated in serum containing DMEM media until 80% confluency was reached. Time based internalization studies were performed for the same conditions and concentrations as the binding studies. All the coverslips were fixed with 2% paraformaldehyde for 15 min and washed with 1x PBS post-fixation before mounting on the slides using ibidi mounting media (DAPI staining). All the slides were analyzed with a Nikon AIR-MP microscope under the DAPI and Cy5.5 channels.
Air mp microscope
Manufactured by Nikon
The AIR-MP microscope is a high-performance optical microscope designed for laboratory use. It features a motorized stage and advanced optics to enable precise sample observation and analysis. The microscope's core function is to provide clear, high-resolution imaging of specimens for scientific and research applications.
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Lab products found in correlation
2 protocols using air mp microscope
1
Time-Dependent Cell Internalization Assay
2
Multimodal Microscopy Characterization
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Scanning electron microscopy and electron microprobe (EDS) analysis were performed with a Zeiss Merlin field-emission SEM instrument. Transmission electron microscopy images were collected with a Zeiss Libra 120 EFTEM. Confocal laser scanning microscopy (CLSM) imaging of bacterial biofilms was performed with a Nikon AIR MP microscope equipped with a 60×, NA 1.4, oil immersion phase-contrast objective (excitation wavelength 488 nm).
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