The following antibodies were used in this study: mouse anti-FLAG (M2) from Sigma (RRID:AB_439685), rabbit anti-human PSAP antibodies from Proteintech Group (RRID:AB_2172462), goat anti-human PGRN (RRID:AB_2114489), and sheep anti-mouse PGRN (RRID:AB_2114504) from R&D systems, rat anti-mouse LAMP1 (1D4B) from BD Biosciences (RRID:AB_2134499). Rabbit anti-human saposin B antibodies (Leonova et al., 1996 (link)) is a gift from Dr. Xiaoyang Qi (University of Cincinnati School of Medicine, Cincinnati, OH).
Rat anti mouse lamp1 1d4b
Manufactured by BD
Rat anti-mouse LAMP1 (1D4B) is a primary antibody that recognizes the lysosome-associated membrane protein 1 (LAMP1) in mouse samples. LAMP1 is a glycoprotein found in the membrane of lysosomes and late endosomes. This antibody can be used in various laboratory techniques to detect and study LAMP1 expression.
Automatically generated - may contain errors
Lab products found in correlation
Sheep anti mouse pgrn, by R&D Systems (1 mentions)
Mouse anti flag m2, by Merck Group (1 mentions)
Goat anti human pgrn, by R&D Systems (1 mentions)
Mouse anti beta 3 tubulin, by Promega (1 mentions)
Mouse anti v5, by Thermo Fisher Scientific (1 mentions)
Mouse anti gapdh, by Proteintech (1 mentions)
3 protocols using rat anti mouse lamp1 1d4b
1
Antibody Characterization for Protein Studies
2
Antibody Characterization in Cell Studies
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The following antibodies were used in this study: rat anti-mouse LAMP1 (1D4B) from BD Biosciences, goat anti-mouse Cathepsin D (C-20) from Santa Cruz and mouse anti-beta III tubulin from Promega. Rabbit anti-subunit c of mitochondrial ATP synthase (SCMAS) antibodies [32 (link)] were a gift from Dr. Elizabeth F. Neufeld (David Geffen School of Medicine, University of California, Los Angeles, CA). Rabbit anti-TMEM106B antibodies were generated against the cytosolic domain of human TMEM106B as previously described [6 (link)].
3
Antibody Characterization for Cell Biology
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The following antibodies were used in this study: mouse anti-FLAG (M2) and rabbit anti-LRP1 from Sigma-Aldrich, rabbit anti-PDI from Thermo Fisher Scientific, mouse anti-GAPDH from Proteintech Group, mouse anti-V5 from Invitrogen, and rat anti–mouse LAMP1 (1D4B) from BD. Sheep anti–mouse PGRN and goat anti–human PGRN antibodies were obtained from R&D Systems. Rabbit anti–mouse PSAP and PGRN antibodies were generated by Pocono Rabbit Farm and Laboratory using the recombinant Gst-PSAP proteins purified from bacteria or FLAG-tagged PGRN from HEK293T cells as the antigen. Rabbit anti-–human saposin B antibodies (Leonova et al., 1996 (link)) and goat anti–mouse PSAP antibodies (Sun et al., 2005 (link)) were described previously. Rabbit anti–cathepsin D and anti-M6PR antibodies were homemade by W. Brown’s laboratory (Cornell University, Ithaca, NY; Park et al., 1991 (link)). The homemade anti-TMEM106B antibodies have been characterized previously (Brady et al., 2013 (link)).
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