Srl india

All chemicals used in the study were of analytical grade and were either procured from an Indian manufacturer (SRL India, HiMedia chemicals) or obtained from Sigma Aldrich (St Louis, MO), Thermo Fisher Scientific Inc (USA) etc. Mannan oligosaccharide (MOS), 1,3,5-trihydroxybenzene, phloroglucinol, D-xylose, ethylene diaminetetracetic acid (EDTA), BCA kit, hematoxylin, eosin, thiobarbituric acid, BSA, Mops, Sucrose, TBA, DTNB, NEM, neutral buffered formalin, decalcifying solution-Lite, were obtained from Sigma-Aldrich Chemical Co., St. Louis, MO, USA. Phosphate buffer saline (PBS), acetic acid, hydrochloric acid, and all other chemicals obtained were of analytical grade from SRL India. Reagents for hematology analyzer (Isotonac 3, Hemolynac 5, Hemolyzing Reagent, Hemolynac 3, Cleanac 3) were procured from Nihon Kohden, Japan.

All chemicals used in study were of analytical grade and were either procured from Indian manufacturer (SRL India, HiMedia chemicals) or obtained from Sigma Aldrich (St Louis, MO), Thermo Fisher Scientific Inc. (USA) etc. Roswell Park Memorial Institute (RPMI-1640) medium, High glucose Dulbecco’s Modified Eagle’s medium (DMEM), Eagle’s Minimum Essential Medium (EMEM), penicillin, streptomycin, blasticidin, trypsin, mannan, rotenone, antimycin A, bovine serum albumin (BSA), ethidium bromide, sodium pyruvate, uridine, protease and phosphatase inhibitor cocktails, REDTaq ReadyMix PCR reaction mix, antibodies (anti MnSOD, anti-SOD1, phospho JNK, phospho p38, anti rabbit/ mouse-HRP, anti β-actin-HRP) etc. were obtained from either Sigma Aldrich (St Louis, MO) or Cell Signaling technology, USA, whereas 3, 3’-DihexyloxacarbocyanineIodide [DiOC₆ (3)], JC-1, 5-(and-6)-chlormethyl 2′,7’dichlorodihydrofluorescein diacetate acetyl ester [CM-H₂DCFDA], 10-N-nony1–3, 6-bis [dimethylamino] acridine (NAO), Dihydroethidine (DHE), sulphorhodamine-B (SRB), fetal bovine serum (FBS), Keratinocyte-SFM (1X) were procured from Thermo Fisher Scientific Inc. (USA).