Nod scid or nci scid ncr athymic nude mice

Animal studies were approved by the Institutional Animal Care and Use Committee (IACUC) at The University of Pennsylvania and/or The University of Michigan. Animal use and care was in strict compliance with institutional guidelines and all experiments conformed to the relevant regulatory standards by the Universities. The maximal tumor size in our study models (mice) never exceeded 1 cm in diameter as allowed by the above ethics committee. NOD SCID or NCI SCID/NCr athymic nude mice were obtained from the Jackson Laboratory (strain code: 005557) and Charles River (strain code: 561). Prostate-specific Pten knockout mice were generated by crossing Pbsn-Cre male mice Tg (Pbsn-cre) 4Prb/J (Jackson laboratory, Strain: 026662) with Pten floxed mice (B6.129S4-Ptentm1Hwu/J, Strain: 006440). All mice were housed in a pathogen-free animal barrier facility and all in vivo experiments were initiated with male mice aged 5-8 weeks.
The maintenance of mice and the experimental procedures (immunizations) have been conducted according to the Austrian Animal Experiments Act and have been approved by the Austrian Federal Ministry of Science and Research BMWFW-66.009/0211-WF/V/3b/2015 and the animal experiments ethics committee of the Medical University of Vienna.

Animal studies were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Pennsylvania and/or the University of Michigan. Animal use and care were in strict compliance with institutional guidelines, and all experiments conformed to the relevant regulatory standards by the universities. NOD SCID or NCI SCID/NCr athymic nude mice were obtained from the Jackson Laboratory (strain code: 005557) and Charles River (strain code: 561). All mice were housed in a pathogen-free animal barrier facility, and all in vivo experiments were initiated with male mice aged 5–8 weeks.