ATRA (Sigma-Aldrich, St. Louis, MO, USA), dissolved in dimethyl sulfoxide, and cytarabine (Sigma-Aldrich), dissolved in water, were stored as 1 mM stock solutions in small aliquots at −20 °C. Tranylcypromine (TCP), a nonreversible LSD1 inhibitor purchased from Sigma-Aldrich, was prepared as a 10 mM stock solution in dissolved dimethyl sulfoxide and stored at −20 °C.
Tranylcypromine tcp
Manufactured by Merck Group
Sourced in United States
Tranylcypromine (TCP) is a monoamine oxidase inhibitor (MAOI) compound used as a laboratory reagent. It is primarily utilized in research and development applications to understand the role of monoamine oxidase enzymes in biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Cytarabine, by Merck Group (1 mentions)
Nifedipine, by Merck Group (1 mentions)
Picrotoxin, by Merck Group (1 mentions)
Bicuculline, by Merck Group (1 mentions)
Mefloquine, by Merck Group (1 mentions)
D apv, by Merck Group (1 mentions)
Ory 1001, by Cayman Chemical (1 mentions)
Ns 398, by Merck Group (1 mentions)
Ah6809, by Merck Group (1 mentions)
Al 8810, by Merck Group (1 mentions)
3 protocols using tranylcypromine tcp
1
Stock Preparation of Anticancer Agents
2
Synaptic and Ion Channel Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Synaptic and ion channel inhibition experiments were performed with bicuculline (10 μM, Cat# 14340, Sigma), picrotoxin (10 μM, Cat# P1675, Sigma), NBQX (10 μM, Cat# N183, Sigma), D-APV (40 μM, Cat# A8054, Sigma), Nifedipine (10 μM, Cat# N7634, Sigma), TTX (1 μM, Cat# 1078, Tocris) and Mefloquine (25 μM, Cat# M2319, Sigma). The rescue experiments were performed with Tranylcypromine (TCP, 600 nM, Sigma, P8511) and ORY-1001 (0.6–0.9 nM, Cayman Chemical, 19136). All the compounds were added to the culture media as described in the results.
3
Preparation and Storage of Vasoactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetylcholine chloride (ACh), noradrenaline hydrochloride (NA), AH-6809 (No.: 33458-93-4), AL-8810 (No.: 246246-19-5), NS-398 (No.: 123653-11-2), and tranylcypromine-TCP (No.: 1986-47-6) were shipped from Sigma-Aldrich (St. Louise, MO, USA). Stock solutions (10 mM) of AH-6809, AL-8810, NS-398, TCP were dissolved in DMSO. Noradrenaline was dissolved in a NaCl + ascorbic acid (0.9% + 0.01% w/v) solution. The solvent concentration was of less than 0.01% (v/v). These solutions were stored at −20 °C, and appropriate dilutions were made in Krebs–Henseleit solution (KH in mM: NaCl 115; CaCl2 2.5; KCl 4.6; KH2PO4 1.2; MgSO4 1.2; NaHCO3 25; glucose 11.1) on the day of the experiment [13 (link)]. Copper carbonate of ≥99% purity was obtained from Poch (Gliwice, Poland).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!