The surface resonance plasmon absorption transition of ZnNPs was performed with 1 cm quartz cuvettes using the spectrophotometer (UV-260, Shimadzu Corp. Tokyo, Japan) and the absorbance was read in the wavelength of 200–700 nm.
Uv 260
Manufactured by Shimadzu
Sourced in Japan
The UV-260 is a UV-Vis spectrophotometer designed for general laboratory use. It features a wavelength range of 190-1100 nm and can perform absorbance measurements. The instrument is equipped with a deuterium lamp for the UV range and a tungsten-halogen lamp for the visible range.
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Lab products found in correlation
8 protocols using uv 260
1
ZnNPs Surface Plasmon Absorption
2
Protein and Nucleic Acid Leakage Measurement
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Protein and nucleic acid leakage was performed according to Li et al [20] (link). Supernatants (8000 × g, 4 °C, 10 min) of L. monocytogenes were collected. An UV spectrophotometer (UV-260, Shimadzu, Tokyo, Japan) was used to determine absorbance at 260 and 280 nm, which represent the highest absorption peaks of proteins and nucleic acids, respectively.
3
Spectrophotometric Quantification of GSH
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GSH content was measured by the method of Moron et al., [35 (link)]. Briefly, proteins were precipitated by 25% TCA, centrifuged and the supernatant was collected. The supernatant was mixed with 0.2 mol sodium phosphate buffer pH 8.0 and 0.06 mmol DTNB and incubated for 10 min at room temperature. The absorbance of the sample/s was read against the blank at 412 nm in a UV-Visible Spectrophotometer (Shimadzu UV-260, Shimadzu Corp., Tokyo, Japan) and the GSH concentration has been calculated from the standard curve.
4
Comprehensive Characterization of Fabricated CQDs
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The functional properties of the fabricated CQDs were analysed by FTIR Spectroscopy (Nicolet iS10). Gemini model 18 SUPRA 55VP-ZEISS Oberkochen (Germany) FESEM model with energy-dispersive X-ray 19 spectroscopy was used to get the morphological surface mapping of CQD (EDX). Scanning electron microscopy was used to examine the surface morphology (Hitachi S-3400N), and transmission electron microscopy (Thermo Fisher; Talos 120C) was used to acquire the nanostructure, including the CQDs' particle size. The optical properties of the material and the degradation potentials of the pollutants were determined using UV–vis spectrophotometry (UV260, Shimadzu) over a range of 200–800 nm. A nano-zeta potential analyser (Malvern) was used to examine the hydrodynamic characteristics of CQDs. The emission was examined using photoluminescence spectroscopy (FLS920 Edinburgh Instrument), and a detection range of 200–1000 nm was used to acquire the excitation light. Lastly, the elemental composition of the CQDs was obtained using the energy-dispersive X-ray analysis (Apollo X). The adsorbent's structure, composition and material properties were obtained using XRD DIFFRAC EVA software and Bruker AXS D8 Advance Karlsruhe Germany. Unless otherwise stated, all measurements were made in ambient circumstances.
5
Quantitative Carbohydrate Determination
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About 1.0 g of plant material was weighed and crushed with a pestle in a mortar with 10 mL of DW. This was transferred into a centrifuge tube and centrifuged for 10 minutes at 10 000 rpm. The supernatant was collected, then 1 mL of this solution was pipette out into a test tube, 4 mL freshly prepared 0.2% anthrone (Sisco Research Laboratories) was added to it and the mixture heated in a boiling water bath for 10 minutes at 100°C. This was allowed to cool to room temperature. The absorbance of the developed green to dark green color was measured at 630 nm using UV‐vis double beam spectrophotometer (UV‐260; Shimadzu, Tokyo, Japan) [20 ].
6
Cell Viability Assay of LFs
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LFs were cultured in 96-well plates (5×105/well; 100 µl) in serum-free DMEM for 24 h at 37°C. CCK-8 solution (cat. no. C0038; Beyotime Institute of Biotechnology, Haimen, China) was added to the wells (10 µl/well) for 1 h at 37°C. Absorbance was measured at 450 nm using an ELISA reader (UV-260; Shimadzu Corporation, Kyoto, Japan).
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LF Proliferation Assay with EGF/PD98059
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LFs were cultured for 24 h in serum-free DMEM and then divided into the four treatment groups in 24-well plates (5 × 105 cells/100 μL/well). LFs were incubated for 6, 12, or 24 h with EGF, PD98059, or EGF+PD98059 at 37°C. Cell counting kit-8 (CCK-8; #C0038; Beyotime, Shanghai, China) was added to each well (10 μL/well) for the final 1 h. The absorbance was calculated at 450 nm by enzyme-linked immunosorbent assay reader (Shimadzu UV-260, Kyoto, Japan).
8
Culturing E. coli and S. aureus for Experiments
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The Escherichia coli ATCC 25250 and Staphylococcus aureus ATCC 6020 strains were stored at 5℃ in tyrptic soy agar (TSA) containing a solution of pancreatic digest of casein 15 g/L, enzymatic digest of soybean meal 5 g/L, sodium chloride 5 g/L and agar 15 g/L (Merck, USA) . Before use, one colony of bacteria was transferred to 5 mL tryptic soy broth (TSB) composed of the following: pancreatic digest of casein 17.0 g/L, enzymatic digest of soybean meal 3.0 g/L, dextrose 2.5 g/L, sodium chloride 5.0 g/L, and potassium phosphate 2.5 g/L, and was cultured for 24 hours at 37℃. Bacterial suspensions were diluted to desired concentration by measurements of the optical density of the suspensions at 600 nm using a spectrophotometer (UV 260, Shimadzu, Japan) with reference to standard values established by a ten-fold dilutions of the suspension on TSA plates. Purity of the working culture was determined by gram staining 33) .
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