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[3H]-H2O is a radiolabeled chemical compound, where the hydrogen atoms in water are substituted with the radioactive isotope tritium (3H). This product is primarily used as a tracer in various scientific and research applications to study water movement, labeling, and other processes that involve water.

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Lab products found in correlation

U 14c glucose, by PerkinElmer (1 mentions) N hexane, by Merck Group (1 mentions) 2 propanol, by Merck Group (1 mentions) 1 n h2so4, by Merck Group (1 mentions)

2 protocols using 3h h2o

1

In Vivo Metabolic Tracing

Cited 1 time
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Ad lib-fed or sucrose re-fed mice received a bolus of 5mCi of [3H]-H2O (American Radiolabeled Chemicals) and 10μCi of [U-14C]-glucose (Perkin Elmer), and were sacrificed 1h later. Lipids were extracted from tissue samples and rates of incorporation of 3H and 14C tracers were calculated as described (Herman et al., 2012 (link)).
Vijayakumar A., Aryal P., Wen J., Syed I., Vazirani R.P., Moraes-Vieira P.M., Camporez J.P., Gallop M.R., Perry R.J., Peroni O.D., Shulman G.I., Saghatelian A., McGraw T.E, & Kahn B.B. (2017). Absence of Carbohydrate Response Element Binding Protein in Adipocytes Causes Systemic Insulin Resistance and Impairs Glucose Transport. Cell reports, 21(4), 1021-1035.
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2

Plasma Triglyceride Extraction Protocol

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Ad libitum-fed mice were i.p. injected with 1 mCi 3H-H2O (American Radiolabeled Chemicals, St. Louis, Missouri) and sacrificed with i.p.160 mg/kg ketamine (Streuli Pharma, Uznach, Switzerland) and 24 mg/kg xylazine (Streuli Pharma, Uznach, Switzerland). For triglyceride extraction, plasma samples were mixed with 1 mL of 2-propanol (Merck, Burlington, Massachusetts) /n-hexane (Merck, Burlington, Massachusetts) /1 N H2SO4 (Sigma-Aldrich, St. Louis, Missouri) (4:1:1) and incubated for 30 min. ddH2O and n-hexane were added and the resulting n-hexane phase was collected, dried, and counted by a scintillation counter.
Shimobayashi M., Thomas A., Shetty S., Frei I.C., Wölnerhanssen B.K., Weissenberger D., Vandekeere A., Planque M., Dietz N., Ritz D., Meyer-Gerspach A.C., Maier T., Hay N., Peterli R., Fendt S.M., Rohner N, & Hall M.N. (2023). Diet-induced loss of adipose hexokinase 2 correlates with hyperglycemia. eLife, 12, e85103.
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