Adhesive silane mas coated glass slide

Three, 7 and 28 days post-operatively, mice were anesthetized with 40 mg/kg pentobarbital sodium and euthanized by transcardial perfusion with cold phosphate buffered saline (PBS). L3-5 lumbar spinal cord segments were harvested and immediately embedded in a pre-cooled solution of 2% carboxymethyl cellulose (CMC) sodium salt (Wako, Osaka, Japan), and then frozen in powdered dry ice. The solid tissues embedded in CMC were stored at −80 °C until sectioning. Tissues were sliced into 10 μm thick axial serial sections with a cryostat (CM1950; Leica, Wetzler, Germany). Tissue sections from sham and SNT mice were mounted onto the same indium-tin-oxide (ITO)-coated glass slides (Bruker Daltonics, Billerica, MA, USA) for MALDI-IMS analysis, and consecutive sections of those were also mounted onto the Matsunami Adhesive Silane (MAS)-coated glass slide (Matsunami, Osaka, Japan) for immunohistochemistry. All the sections were stored at −80 °C until matrix application.

The samples from cases 1, 4, and 7 were embedded in 2 % CMC at −80 °C before sectioning, and the other samples were sectioned without embedding. The tissue blocks were sliced to a thickness of 10 μm at −20 °C with a cryostat (CM1950; Leica, Wetzler, Germany). The consecutive tissue sections were mounted on an indium tin oxide (ITO)-coated glass slide (Bruker Daltonics) and a Matsunami Adhesive silane (MAS)-coated glass slide (Matsunami, Osaka, Japan) for the IMS analysis and HE staining, respectively. All slides with sample sections were stored at −20 °C until their use in the analyses.