Tris tricine gel
Tris-Tricine gels are a type of polyacrylamide gel electrophoresis (PAGE) system used for the separation and analysis of proteins, particularly low-molecular-weight proteins. These gels utilize a Tris-Tricine buffer system, which provides improved resolution and separation of proteins compared to traditional Tris-glycine gels. Tris-Tricine gels are commonly used in applications such as protein profiling, molecular weight determination, and analysis of protein expression patterns.
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11 protocols using tris tricine gel
Western Blot Analysis of Amyloid Plaques and ApoE
Western Blot Analysis of Amyloid Plaques and ApoE
Protein Extraction and Western Blot Analysis
For Pol II blots, home-made 6% acrylamide gels (37.5:1 acrylamide:bis-acrylamide ratio) were prepared and run for 1 h at 30 mA per minigel and transferred to 0.4-µm nitrocellulose membranes in standard Laemmli TB for 2 h at 200 mA with an ice block.
For histone blots, premade 12% Bolt Tris–tricine gels (Novex) were run in proprietary Novex MES running buffer according to the manufacturer’s manual and transferred to 0.2-µm nitrocellulose membranes in Novex transfer buffer for 1 h at 200 mA with an ice block.
After transfer, all membranes were blocked with 2% milk in TBS-T (50 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween-20), incubated overnight with primary antibodies (Supplementary Table
Protein Expression Analysis in Mycobacteria
Western Blot Analysis of Amyloid-Beta in CSF and Brain
Analyzing γ-Secretase Activity in PS1 Mutants
Photocrosslinking of RNA Polymerase
Amyloid-beta detection by western blot
Amyloid-Beta Protein Analysis by Western Blot
Western Blot Analysis of Amyloid-β Aggregation
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