We measured hs-cTnT on stored blood samples collected during Visit 4 (1996–1998) with a high-sensitivity assay, Elecsys Troponin T (Roche Diagnostics®), on an automated Cobas e411 analyzer.25 (link) We used hs-cTnT cutoffs based on prior studies, with the limit of detection of 5 ng/L.25 (link),42 (link), Similarly, we also measured NT-proBNP on the automated Cobas e411 analyzer (Roche Diagnostics) using an electrochemiluminescent immunoassay with a measurement range of 5–35,000 pg/mL and a limit of quantification of 35 pg/mL.26 (link)
Cobas e411 analyzer
Manufactured by Roche
Sourced in Germany, Switzerland, United States, Japan, France
The Cobas e411 analyzer is a fully automated, random-access immunoassay analyzer designed for clinical diagnostics. It is capable of performing a variety of immunoassay tests to aid in the diagnosis and monitoring of various medical conditions.
Automatically generated - may contain errors
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Cobas c311 analyzer, by Roche (3 mentions)
Elecsys troponin t, by Roche (3 mentions)
Elecys 2010 analyzer, by Roche (3 mentions)
Modular ppe, by Roche (3 mentions)
Cobas e411, by Roche (2 mentions)
Elecsys anti sars cov 2 s immunoassay, by Roche (2 mentions)
Elecsys anti sars cov 2, by Roche (2 mentions)
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Elecsys nt probnp 2 kits, by Roche (2 mentions)
Advia 2120 hematology system analyzer, by Bayer (2 mentions)
Cobas integra 400 plus analyzer, by Roche (2 mentions)
Cobas e601 analyzer, by Roche (2 mentions)
Electrochemiluminescence technology, by Roche (2 mentions)
256 protocols using cobas e411 analyzer
1
Quantifying Cardiac Biomarkers in Blood Samples
2
Cardiometabolic Risk Biomarker Profiling
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Information on medical history, medication use, alcohol use, and current smoking was obtained using standardized self‐report questionnaires. Systolic and diastolic BP measurements were recorded as the mean of 2 readings. Body mass index was calculated as weight in kilograms divided by the square of height in meters, and obesity was defined as body mass index ≥30 kg/m2. Serum glucose was measured using the hexokinase method. Plasma glucose was measured using the hexokinase method. Serum, triglycerides, and high‐density lipoprotein cholesterol concentrations were measured by using automated enzymatic assays. eGFR was calculated from serum creatinine and cystatin C–based new equation.
19 (link) NT‐proBNP (N‐terminal pro‐B‐type natriuretic peptide) was measured using an electrochemiluminescent immunoassay on an automated Cobas e411 analyzer (Roche Diagnostics, Mannheim, Germany), and high‐sensitivity cardiac troponin T levels were measured with a highly sensitive assay, Elecsys Troponin T (Roche Diagnostics, Indianapolis, IN) on an automated Cobas e411 analyzer. CRP (C‐reactive protein) was measured via immunophelometric assay.
19 (link) NT‐proBNP (N‐terminal pro‐B‐type natriuretic peptide) was measured using an electrochemiluminescent immunoassay on an automated Cobas e411 analyzer (Roche Diagnostics, Mannheim, Germany), and high‐sensitivity cardiac troponin T levels were measured with a highly sensitive assay, Elecsys Troponin T (Roche Diagnostics, Indianapolis, IN) on an automated Cobas e411 analyzer. CRP (C‐reactive protein) was measured via immunophelometric assay.
3
S100 and NSE Quantification in CSF and Serum
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CSF and serum S100 concentration was tested on the COBAS e411 analyzer (Roche Diagnostics, Penzberg, Germany) according to the manufacturer’s instructions. The assay is dedicated to the quantitative determination of S100 (S100 A1B and S100 BB) in human samples. Detection range is between 0.005 and 39 µg/l. The test is unaffected by icterus (bilirubin < 680µmol/l or <40 mg/dl), hemolysis (hemoglobin <0.621 mmol/l or <1.0 g/dl), lipemia (lipid <1500 mg/dl), and biotin (<205 nmol/l or <50 ng/ml).
CSF and serum NSE concentrations were tested on the COBAS e411 analyzer (Roche Diagnostics, Penzberg, Germany) according to the manufacturer’s instructions. Detection range is between 0.050 and 370 ng/ml. The cytokines: IL-1α, −1β, −2, −3, −4, −8, interferon-ɣ, and tumor necrosis factor-α in the concentration of 50 ng/ml do not show cross-reactions. The assay is unaffected by icterus (bilirubin <1231 µmol/l or <72 mg/dl), hemolysis (hemoglobin <0.621 mmol/l or <1.0 g/dl), lipemia (triglycerides <22.8 mmol/l or <2000 mg/dl), and biotin (<409 nmol/l or <100 ng/ml).
CSF and serum NSE concentrations were tested on the COBAS e411 analyzer (Roche Diagnostics, Penzberg, Germany) according to the manufacturer’s instructions. Detection range is between 0.050 and 370 ng/ml. The cytokines: IL-1α, −1β, −2, −3, −4, −8, interferon-ɣ, and tumor necrosis factor-α in the concentration of 50 ng/ml do not show cross-reactions. The assay is unaffected by icterus (bilirubin <1231 µmol/l or <72 mg/dl), hemolysis (hemoglobin <0.621 mmol/l or <1.0 g/dl), lipemia (triglycerides <22.8 mmol/l or <2000 mg/dl), and biotin (<409 nmol/l or <100 ng/ml).
4
Cardiac Biomarker Measurement Protocols
5
Cortisol and ACTH Immunoassay Validation
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Cortisol was determined using an immunoassay (Elecsys Cortisol II assay, Cobas e411 analyzer, Roche Diagnostics GmbH, Mannheim, Germany), with intra- and inter-assay CV of 1.8–7.1% and 2.7–12.7%, respectively. ACTH was also determined using an immunoassay (Elecsys ACTH assay, Cobas e411 analyzer, Roche Diagnostics GmbH, Mannheim, Germany) with intra- and inter-assay CV of 2.0–2.9% and 2.4–5.4%, respectively.
6
Routine Clinical Chemistry Analysis
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Measurements of the plasmatic AST and ALT activities, and concentrations of glucose, cholesterol, proteins and urea, were determined using the Cobas e411 analyzer (Roche Diagnostics, Mannheim, Germany), according to the controls and calibration of the Cobas e411 analyzer (Roche Diagnostics, Mannheim, Germany).
7
Fasting Lipid and Insulin Profiling
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During the lab visit, blood samples were collected by a trained nurse after a fasting period of 8–10 h. The levels of fasting glucose and lipid profile (including high-density lipoprotein (HDL), total cholesterol (TC), and TG levels) were measured using the VITROS ECi Immunodiagnostic System and Roche COBAS e411. Low-density lipoprotein (LDL) cholesterol was calculated using the Friedewald formula (LDL = TC-(HDL + 0.20 × TG)) [38 ]. The Roche COBAS e411 Analyzer was used to measure insulin levels.
8
Detailed Biochemical Profiling in PCOS
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Plasma glucose, TC, TGs, HDL-C, and LDL-C were measured by appropriate enzymatic methods on the DP Modular Analytics (Roche Diagnostics). Fasting insulin and SHBG were measured using Immulite 1000 Analyzer (Siemens Healthcare Diagnostics). Serum LH, FSH, total estradiol, and total testosterone levels were measured by electro-chemiluminescent immunoassays on the Roche E170 system (Roche Diagnostics). Serum total testosterone at follow-up was measured by the liquid chromatography tandem mass spectrometry (LC-MS/MS) method (Waters Quattro Micro) [13 ]. Based on the results of 170 archived samples from women with PCOS measured using both platforms and on the Passing and Bablok regression analysis conducted [13 ], a conversion calculation was performed on the follow-up testosterone levels to facilitate a comparison between the baseline and follow-up total testosterone results. AMH was measured using Elecsys AMH electro-chemiluminescent immunoassay Roche Cobas E411 analyzer (Roche Diagnostics). These measurements were performed using standard reagent kits supplied by the manufacturers. The analytical performance of these assays was within the specifications of the analysers.
9
Quantifying SARS-CoV-2 S Antibodies via ECLIA
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The Elecsys anti-SARS-CoV-2 S test (Roche S-RBD tAb) was used to assess total anti-SARS-CoV-2 S antibody levels. This test is based on the electrochemiluminescence immunoassay (ECLIA) which is used for in vitro quantitative determination of total antibodies (IgG/IgA/IgM) against the SARS-CoV-2 S-RBD protein in the human serum. The assay was conducted with the use of the fully automated Roche Cobas E411 analyzer (Roche Diagnostics). The ECLIA test is a three-step procedure that uses a recombinant protein representing the receptor binding domain (RBD) of the S antigen in a double-antigen assay format and favors the detection of high-affinity antibodies against SARS-CoV-2. Serum samples are incubated with a mix of biotinylated and ruthenylated RBD antigens to produce double antigen immune-complexes. Streptavidin-coated microparticles are then added, and DAGS complexes bind to the solid phase. The reagent mixture is transferred to the measuring cell where the microparticles are magnetically captured. Electrochemiluminescence is initiated by applying voltage, and it is measured with a photomultiplier. Signal yield increases with the antibody titer. The test has a measuring range of 0.40–250 U/mL (up to 25,000 U/mL in a 1:100 dilution), and concentrations below 0.80 U/mL are regarded as negative, whereas concentrations of ≥0.80 U/mL are regarded as positive.15
10
Biochemical Markers of Fatigue
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Fatigue-associated biochemical indices in the serum were determined using an autoanalyzer (SYSMEX XT-2000iv, Sysmex corp., Kobe, Japan). The effects of RC on serum lactate, ammonia, creatine kinase (CK), glucose, lactate dehydrogenase (LDH), and FFA activity were evaluated postexercise. One hour after the last dose of the 41-day supplementation, a 15-min swimming test was performed without weight loading. After 1 h of the last intragastric administration of RC, in all mice serum samples were collected by cardiac puncture and analyzed using an autoanalyzer (SYSMEX XT-2000iv, Sysmex corp., Kobe, Japan). The hormones E2 and progesterone (PGN) were determined using the Roche Cobas e411 analyzer (Roche Diagnostics, Mannheim, Germany).
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