C57bl 6 b6

C57BL/6 (B6) and B6 Spp1^−/− mice were purchased from the Jackson Lab. Sex- (male or female) and age- (6 to 7 week old) matched animals were used for all the experiments. All mice were maintained in barrier facilities and used according to Duke University Institutional Guidelines. This study was approved by the Duke University Institutional Animal Care and Use Committee.

C57BL/6 (B6) and Bm12 mice were purchased from Jackson Laboratories. The il33^−/− mice were a gift from S. Nakae (University of Tokyo, Tokyo, Japan)(27 ). St2^−/− mice were originally generated on a BALB/c background as described(28 (link)) and obtained from Dr. Anne Sperling (University of Chicago) after they were backcrossed 7 times onto the C57BL/6 background. These mice were then backcrossed 3 additional times onto the C57BL/6 background here at the University of Pittsburgh before use in our experiments. Animals were housed in a specific pathogen-free facility maintained by the University of Pittsburgh. The studies conformed to the principles set forth by the Animal Welfare Act and the National Institutes of Health guidelines for the care and use of animals in biomedical research.

G0s2^−/− mice were generated in our laboratory using embryonic stem cells with targeted deletion of the G0S2 gene (VelociGene, Regeneron), purchased from the Knockout Mouse Project (KOMP) repository. The embryonic stem cell line VGB6 (C57BL/6NTac strain) contains the ZEN-Ub1 cassette inserted in the G0s2 gene. Embryonic stem cells were injected into the blastocoels of 3.5 d.p.c. C57BL/6 albino mouse embryos in the Genetically Engineered Mouse Core at Baylor College of Medicine. Chimeras were crossed to C57BL/6 albino mice to assess germline transmission, and then heterozygous mice were backcrossed to C57BL/6 and intercrossed to establish a colony of G0s2^−/− mice. For the infection model, we bred mice with OT-I transgenic mice to generate OT-I G0s2^—/— mice. Mice were genotyped by PCR using tail genomic DNA. Heterozygous females and homozygous males were used for the generation of null mice because G0s2^−/− females failed to nourish newborn pups. C57BL/6 (B6) and B6.SJL mice were purchased from The Jackson Laboratory (Bar Harbor, Maine, U.S.A.). Mice were maintained under specific pathogen-free conditions at the Baylor College of Medicine. All experiments were performed with the approval of the Institutional Animal Care and Usage Committee of Baylor College of Medicine.

Seven to ten-week old male C57BL/6 (B6) and C57BL/6-Tg(CLEC4C-HBEGF)956Cln/J (BDCA2-DTR) (JAX stock #014176) mice were purchased from The Jackson Laboratory. CD1d−/− and Jα18−/− mice were originally generated by Van Kaer and Taniguchi (30 (link), 31 (link)), respectively, and were kindly provided initially by Dr. Mitch Kronenberg (La Jolla Institute for Allergy and Immunology, La Jolla, CA). All mice were maintained in specific pathogen-free conditions.