Small interfering RNA (siRNA) targeting APJ (siRNA-APJ, sequence: CTGACATGTTACTTCTTCA), siRNA-APJ negative control (NC), miR-124-3p mimic, NC mimic, miR-124-3p inhibitor, and NC inhibitor were synthesized by RiboBio Co. (Guangzhou, China). CTDSP1 overexpression adenovirus and the CTDSP1 overexpression adenovirus negative control (NC) were synthesized by GeneChem. (Shanghai, China). Neurons were transfected with siRNA-APJ, siRNA-APJ NC, miR-124-3p mimic, NC mimic, miR-124-3p inhibitor, NC inhibitor, CTDSP1 overexpression adenovirus, or CTDSP1 overexpression adenovirus NC using transfection reagent according to the manufacturer’s instructions (RiboBio Co, China). During this period, neurons were incubated in culture medium without the penicillin/streptomycin solution.
Mimic nc
Manufactured by RiboBio
Sourced in China, United States
The Mimic NC is a laboratory instrument designed for the analysis of nucleic acids. It utilizes next-generation sequencing technology to provide high-throughput and accurate data on various nucleic acid samples.
Automatically generated - may contain errors
Lab products found in correlation
Inhibitor nc, by RiboBio (88 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (71 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (38 mentions)
Si nc, by RiboBio (18 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (13 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (13 mentions)
Sh nc, by RiboBio (12 mentions)
Nc mimic, by Thermo Fisher Scientific (8 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (8 mentions)
Nc sirna, by RiboBio (6 mentions)
Antagomir nc, by RiboBio (6 mentions)
Pcdna3, by Thermo Fisher Scientific (5 mentions)
Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (5 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (5 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (5 mentions)
Opti mem, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Si nc, by GenePharma (4 mentions)
Mirna mimic, by Thermo Fisher Scientific (3 mentions)
Pcdna3, by RiboBio (3 mentions)
208 protocols using mimic nc
1
Modulating Neuronal APJ and miR-124-3p
2
Mir-125a-5p Modulates CD4+ T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CD4+ T lymphocytes from mice spleen were plated in 6-well plates at a density of 2 × 106 cells/well using Lipofectamine 3000, following the manufacturer’s protocols. The cells were co-cultured with either mimic Negative Controls (mimic NCs) or miR-125a-5p mimics for 48 h. After transfection, cells were collected for flow cytometry and quantitative real-time PCR (qRT-PCR). The miR-125a-5p mimics, mimic NCs were synthesized by Guangzhou RiboBio Co., Ltd. The oligonucleotide sequences of miR-125a-5p were as follows: 5′-UCCCUGAGACCCUUUAACCUGUGA-3′. The oligonucleotide sequences of mimic NCs were based on the sequences of miRNA in Caenorhabditis elegans (cel-miR-67).
3
miR-146b-5p Modulation in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
miR-146b-5p mimics, miR-146b-5p inhibitor and their negative controls mimic-NC and inhibitor-NC were provided by RiboBio Co., Ltd. (Guangzhou, China) (mimics: 5'-UGAGAACUGAAUUCCAUAGGCUGU-3'; mimic-NC: 5'-UUCUCCGAACGUGUCACGUTT-3'; inhibitor: 5'-ACAGCCUAUGGAAUUCAGUUCUCA-3'; inhibitor-NC: 5'-CAGUACUUUUGUGUAGUACAA-3'). Lipofectamine® 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used for cell transfection. Briefly, cells were seeded in a 12-well plate (1x105 cells per well) and cultured to 80% confluence. After 4-h starvation in serum-free DMEM, a 100 nM transfection mixture containing the mimics, inhibitor or the mimic-NC or inihibitor-NC (1.6 µg/well) and Lipofectamine® 2000 reagent (4 µl/well) was added for 40 min incubation at 37˚C. Fresh medium was replaced at 24 h and transfection efficacy was examined by reverse transcription-quantitative (RT-q)PCR. Subsequent experiments were performed 2 weeks after the transfection experiment.
4
Evaluating miR-43-3p Regulation in ASCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An miR-43-3p mimic, inhibitor, and corresponding negative controls (mimic-NC and inhibitor-NC) were purchased from RiboBio (miR1N0000001-1-5, miR2N0000001-1-5, Guangdong, China). The sequences of these oligonucleotides are shown in Additional file 1 : Table S3. Briefly, ASCs were seeded in six-well plates at 5 × 105 cells/well and then transfected with 50 nM mimic (mimic-NC) or 200 nM inhibitor (inhibitor-NC) using riboFECT CP Reagent (C11055-1, RiboBio) in accordance with the manufacturer’s protocol. After 48 h of incubation, ASCs were harvested for subsequent experiments.
5
Modulating miR-9 Expression in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
miR-9-mimics, anti-miR-9, NC-mimics, anti-NC, ABCC1 (ATP binding cassette subfamily C member 1), siRNA (si-ABCC1) and si-NC were designed and synthesized by RiBoBio. Lipofectamine 2000 was applied for transfection. The lentiviral empty vector (NC-LV) expressing GFP and GFP with high miR-9 (LV-miR-9-5p) were constructed by Systems Biosciences. Virus establishment and cell transduction were carried out in basement membrane cells, and cells were obtained using puromycin (1μg/mL) and sorted using flow cytometry to maintain at 95% of GFP positive rate.
6
Overexpression of circADD2 in ALL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For analyze circRNA overexpression, pcDNA-based circADD2 overexpression vector and pcDNA vector were synthesized by Genepharma (Shanghai, China). Stable transfection in ALL cells was performed according to manufacturer agreement. MiR-149-5p mimics (or miR-149-5p inhibitor) and NC mimics (or NC inhibitor) were obtained from RiboBio (Guangzhou, China) and transfected with Lipofectamine 2000 (Invitrogen, United States).
7
Cultured Cell Transfection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BGC-823 and MKN-45 cells, which were provided by the cell bank of the Chinese Academy of Sciences (Shanghai, China), were cultured under ATCC recommended conditions and supplemented with 10% fetal calf serum. The medium was changed every other day, and the cells were passaged every 3–4 days. The cells in the exponential phase were selected for further experiments. The cultured cells were uniformly seeded in 6-well cultured plates at a density of 3x105/mL (2 mL per well), and cell growth was observed under an inverted microscope. When the cells reached 60–70% confluence, they were detached using 0.25% trypsin (Gibco; Thermo Fisher Scientific, Inc.). tRF-24 mimics, NC mimics, and scramble (RiboBio, Guangzhou, China), were transiently transfected using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.
8
Knockdown of SNHG3 in Prostate Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PC3 or DU145 cells at the confluence of 50%‐80% were collected and transfected with the shRNAs (short hairpin RNAs) specific to SNHG3 (sh‐SNHG3#1 and sh‐SNHG3#2) or sh‐NC (negative control) (Genepharma, SC, CA, USA). Simultaneously, miR‐577 mimics and NC‐mimics were constructed by RiboBio. The pcDNA3.1 targeting SMURF1 and empty pcDNA3.1 vector were purchased from Genechem (Shanghai, China). Transfection was performed with Lipofectamine 3000 (Invitrogen). Relevant sequences were provided in Table S1 .
9
Myomaker-miRNA Interaction Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Myomaker sequences that contain miRNA binding sites were cleaved using SacI/XhoI and cloned into the pmirGLO plasmid (Promega, Madison, WI, USA). We named the recombinant pmirGLO vectors with the Myomaker sequence as pmirGLO-Myomaker, which were also synthesized by TSINGKE (TSINGKE, Beijing, China). Meanwhile, the corresponding information of miRNA sequences was found from miRbase. miR-205 mimics (50 nM) and NC mimics (50 nM) were purchased from RIBOBIO (RIBOBIO, Guangzhou, Guangdong, China). When Hela cell density in a 48-well plate reached 70%, pmirGLO-Myomaker was co-transfected with miRNA mimic into Hela cells using Lipofectamine 3000, according to the manufacturer’s instructions. Cells were collected after 48 h; dual-luciferase activity was measured using the Dual-Luciferase Reporter Assay System kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions.
10
Exosomal miR-223-5p Regulates HVSMC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MiR-223-5p mimics, NC mimics and miR-223-5p inhibitors were purchased from Ribobio (Guangzhou, China). HVSMCs were seed into a 24-well plate at density of 1 × 105 cells/well and cultured overnight. On the next day, the cells were divided into five groups: blank group, NC group, Exos group, miRNA mimics group and miRNA inhibitor + Exos group. The cells in the NC group and miRNA mimics group were, respectively, transfected with NC mimics and MiR-223-5p mimics using Lipofectamine 2000 (Invitrogen, Carlsbad, USA) according to the manufacturer’s instructions. The cells in the Exos group were treated with 100 µg TAO plasma-derived exosomes. The cells in the miRNA inhibitor + Exos group were firstly transfected with miR-223-5p inhibitor using Lipofectamine 2000 and then were treated with 100 µg TAO plasma-derived exosomes. The cells in the blank group were without treatments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!