The mean length of randomly selected eggs (100 eggs/experiment) deposited within 2 h after mating was estimated from the most proximal to the most distal point. Morphological measurements were taken using an OLYMPUS SZX16 stereomicroscope with Olympus cell-Sens Standard imaging software (Olympus cellSens Standard, Version 1.16, Olympus Corporation, Tokyo, Japan).
Cellsens standard
Manufactured by Olympus
Sourced in Japan, United States
CellSens Standard is an image analysis software designed for microscopy applications. It provides essential tools for capturing, processing, and analyzing images of biological samples.
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Lab products found in correlation
Bx53 microscope, by Olympus (5 mentions)
Szx16, by Olympus (3 mentions)
Image pro plus 6, by Media Cybernetics (3 mentions)
Ckx41, by Olympus (2 mentions)
Bx53f, by Olympus (2 mentions)
Ix50 inverted microscope, by Olympus (2 mentions)
Bx41 microscope, by Olympus (2 mentions)
Szx16 stereomicroscope, by Olympus (2 mentions)
Cytoflex flow cytometer, by Beckman Coulter (2 mentions)
Lsm 880 confocal microscope, by Zeiss (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Axio zoom v16, by Zeiss (2 mentions)
Dibutylphthalate polystyrene xylene (dpx), by Merck Group (2 mentions)
Plan fluorite objective, by Olympus (2 mentions)
Bx51 microscope, by Olympus (2 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
Simple stain dab, by Nichirei Biosciences (1 mentions)
Sz2 ilst, by Olympus (1 mentions)
Bp61 scale, by Sartorius (1 mentions)
Cytoselecttm 24 well wound healing assay, by Cell Biolabs (1 mentions)
101 protocols using cellsens standard
1
Egg Morphometrics After Mating
2
Measuring Secondary Branches in Fly Larvae
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L3 larvae (10 to 15 flies) were removed from the medium with tweezers, washed in 1 × PBS and placed on a drop of glycerin on a slide. The slide was then placed on a heating plate at 70 °C for 10 s in order to kill the larvae. Thereafter, the larvae were aligned with the dorsal side facing upwards, covered with a cover-slip and analyzed with an Olympus SZX16 stereomicroscope using cell-Sens Standard imaging software (Olympus cellSens Standard, Version 1.16, Olympus Corporation, Tokyo, Japan). The third thoracic segment of the larvae was measured at a magnification of 20-fold. All images were scaled and saved as Tiff files. These microscopic images were then used to measure the length of secondary branches using ImageJ software (Version 2.1.4.7, Wayne Rasband National Institutes of Health, USA) with NeuronJ plugin (version 1.4.3). First, the images were converted to an 8-bit version and the contrast was increased until the secondary branches were clearly visible (approx. 1.5–2%). All the processed images were saved as tiff files and thereafter analyzed with NeuronJ. Secondary branches were measured using the "tracking" function of NeuronJ. The previously used scaling was used as the basis for the measurement.
3
Osteocalcin Expression in Bone Regeneration
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After antigen retrieval using 0.125% trypsin (Nichirei Bioscience, Tokyo, Japan) and quenching of endogenous peroxidase, the sections were incubated with rabbit polyclonal antibodies against osteocalcin (1:100 dilution; 20277-1-AP; Proteintech, Rosemont, IL, USA) for 90 minutes at room temperature. After washing, the sections were incubated for 30 minutes at room temperature with goat anti-rabbit immunoglobulin G secondary antibody (horseradish peroxidase) (1:500 dilution; 43R-1495; Fitzgerald Industries International, Inc., North Acton, MA). Tissue sections were visualised using 3,3′-diaminobenzidine (Simple Stain DAB; Nichirei Bioscience), followed by counterstaining with haematoxylin for 3 minutes. Images were acquired under the light microscope. We measured the perimeter of the new collagen (on the bone surface) in the area of bone regeneration and the length of the collagen surface with osteocalcin-positive osteoblasts (Olympus cellSens Standard; Olympus). The active osteoblast surface of the decalcified bone (%) was measured in relation to the decalcified bone surface.
4
Floral Primordium Developmental Stages
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The floral primordium of wild-type Williams 82 during five developmental stages (stages 1–5) was observed by scanning electron microscopy. After removing the bracts, they were fixed in a formaldehyde alcohol acetic acid (FAA) fixation solution for at least 12 h. All materials were dehydrated with ethanol (30, 50, 70, 90, 95, 100%) and then dehydrated with ethanol-tert-butanol to 100% tert-butanol. The samples were dissected using a positive microscope and held in small Petri dishes with conductive tape. The floral primordium was scanned using InTouchScope Scanning Electron (JSM-IT500, Japan Electronics, Tokyo, Japan). The anatomical observation of flower organs was performed using an orthomicroscope (SZ2-ILST, Olympus Co., Tokyo, Japan) which was photographed with Olympus cellSens Standard, imaging software (Version 1.16, Olympus Co., Tokyo, Japan).
5
Larval Growth Measurement Protocol
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L1 and L3 larvae (20 larvae/experiment) were extracted from the vials 24 and 72 h, respectively, following egg deposition. Thereafter, the larvae were fixed in 70% ethanol, dried and then either weighed or placed on glass slides for further microscopic analysis. The measurement of the length of L1 and L3 larvae obtained from egg batches laid by e-nicotine- and water-exposed females was carried out from the most proximal to the most distal point of the body with an OLYMPUS SZX16 stereomicroscope using Olympus cell-Sens Standard imaging software (Olympus cellSens Standard, Version 1.16, Olympus Corporation, Tokyo, Japan). The weights of L3 larvae were recorded using Sartorius BP61-scale.
6
Quantitative Analysis of Lesion Microenvironment
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An Olympus BX53 microscope (BX53, Olympus, Tokyo, Japan) was used to observe the maximum cut surface of the lesions.
Fig. 2 shows a schematic diagram of the measurement site on the cut surface just above the lesion.
The spinous and basal cell layers are designated as ① and ②, respectively.
Any point immediately above the lesion was designated as point A, and points on both sides 100 mm from point A were designated as points B and C, respectively. One measurement site was defined at 200 mm between points B and C. Further, 200-mm widths were set on both outer sides on the same section, and a total of three measurement sites were set as measurement ranges.
For epithelial thickness, the thicknesses of ① to ② were measured.
The number of Ki67-positive and negative cells in the basal layer, as well as the number of blood vessels in the surface lamina propria (0e10 mm) and the deep lamina propria (10e100 mm) layers of the measurement site were counted, and the area per blood vessel was measured. In the normal mucosa, the measurement range was three places with a 200-mm margin width. The margin was used as the control group and compared with the area immediately above the lesion. Measurements were taken at three locations, and the average value was used as the numerical value for the case. Measurements were performed using Olympus cell-Sens Standard (Olympus, Tokyo, Japan).
Fig. 2 shows a schematic diagram of the measurement site on the cut surface just above the lesion.
The spinous and basal cell layers are designated as ① and ②, respectively.
Any point immediately above the lesion was designated as point A, and points on both sides 100 mm from point A were designated as points B and C, respectively. One measurement site was defined at 200 mm between points B and C. Further, 200-mm widths were set on both outer sides on the same section, and a total of three measurement sites were set as measurement ranges.
For epithelial thickness, the thicknesses of ① to ② were measured.
The number of Ki67-positive and negative cells in the basal layer, as well as the number of blood vessels in the surface lamina propria (0e10 mm) and the deep lamina propria (10e100 mm) layers of the measurement site were counted, and the area per blood vessel was measured. In the normal mucosa, the measurement range was three places with a 200-mm margin width. The margin was used as the control group and compared with the area immediately above the lesion. Measurements were taken at three locations, and the average value was used as the numerical value for the case. Measurements were performed using Olympus cell-Sens Standard (Olympus, Tokyo, Japan).
7
Wound Healing Assay Using CytoSelect
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We performed wound healing assay using CytoSelect TM 24-Well Wound Healing Assay (CELL BIOLABS, San Diego, CA, USA) following the instructions in the manual. SKOV3 cell suspension is added at a density of 1 × 10 6 cells/well with insert in place and incubated 24 hours. We remove insert to make a wound field of 0.9 mm. And then samples ware incubated for 24 hours. samples were stained using Cell staining (CELL BIOLABS, San Diego, CA, USA) to visualize clearly. We monitored the wound field with microscope and measured the area of migration using OLYMPUS cell-Sens Standard (OLYMPUS, Tokyo, Japan). We repeated three times independently and analyzed the results.
8
Histopathological Evaluation of Liver Granulomas
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The livers were fixed in 10% formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin for histological examination. After staining, the histopathological changes were observed and examined under optical microscope (Olympus X71-F22PH; Olympus Corporation, Tokyo, Japan). For quantification, the total number and size of granulomas in 30 optical fields (200× magnification) of each liver section were determined using the Olympus software (CellSens Standard). The fields were chosen randomly and continuously. Data were presented as mean ± SD of five mice of each group. The pathologist was blinded to all the histopathological examinations.
9
Quantifying Tumor Cells and PD-L1 in NSCLC
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Pathological diagnosis was confirmed by a histopathologist based on hematoxylin-eosin (HE) cell staining. After a confirmed diagnosis of NSCLC, we evaluated the number of tumor cells and sample size of the first consecutive five specimens in the large GS group and small GS cohort. We scanned the HE-stained slides with a scanner (Nano zoomer® 2.0RS, HAMAMATSU, Japan). A pathologist and a cytologist, blinded to the clinical details, manually counted the number of tumor cells and evaluated the proportion of tumor cells in all nuclear cells using imaging software (NDP scan® 2.5, HAMAMATSU, Japan), and average number and proportion assessed by the two evaluators. Only viable tumor cells were counted, and those that were difficult to differentiate from non-tumor cells, such as inflammatory cells, or those with strong degeneration or necrosis were excluded. The sample size was measured by the cumulative area of individual specimens using imaging software (cellSens® standard, Olympus, Japan). PD-L1 was evaluated using the samples containing the largest number of tumor cells obtained by GS-TBB.
10
Body Length Measurement of Daphnia
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One-month-old D. pulex reared under long-day (n = 10) and short-day (n = 11) conditions were each held between a glass slide and glass cover slip, and images were captured using a light microscope (CKX41, Olympus, Tokyo, Japan), CCD camera (DP-72, Olympus) and analysis software (CellSens Standard, Olympus). Body lengths were measured from the top of the head to the proximal region of the tail-spine using ImageJ software26 (link). Measured body-lengths of adults raised under each condition were assessed by Student’s t-test.
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