Five week old BALB/c nude male mice weighing 20–25 g were purchased from Beijing Huafukang Bioscience Co. Inc. (Beijing, China) and used for the in vivo tumor assay. A549 cells (2 × 106) that stably transfected the miR‐195 mimics or NC were suspended in 100 μL serum‐free DMEM/Matrigel (1:1). The cells were then injected subcutaneously into the flank of each mouse. Tumor volumes were measured weekly and calculated using the formula: volume (mm3) = 1/2 (length × width2). The mice were sacrificed by cervical dislocation under anesthesia with diethyl ether after 30 days. The tumor tissues were harvested, weighed, and used to determine miR‐195 and apelin expression. The Animal Ethics Committee of Kunming Medical University (Kunming, Yunnan, China) approved all animal experiments.
Male balb c nude mice
Manufactured by Beijing Huafukang Biotechnology
Sourced in China
Male BALB/c nude mice are laboratory animals commonly used in biomedical research. They are characterized by the absence of a functional immune system, specifically a lack of mature T cells. This characteristic makes them useful for studying the role of the immune system in various disease models and for conducting xenograft studies involving human tumor cells.
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Lab products found in correlation
Sh nc, by Hanbio Biotechnology (1 mentions)
Matrigel, by BD (1 mentions)
Male kunming mice, by Beijing Huafukang Biotechnology (1 mentions)
Coumarin 6, by Merck Group (1 mentions)
Tnf α and il 1β elisa kits, by R&D Systems (1 mentions)
Curcumin, by Merck Group (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Wild type mice, by Beijing Huafukang Biotechnology (1 mentions)
Anti iba1, by GeneTex (1 mentions)
Indocyanine green icg, by Merck Group (1 mentions)
40 protocols using male balb c nude mice
1
Xenograft Tumor Assay of miR-195 in BALB/c Nude Mice
2
Xenograft Mouse Model of Gastric Cancer
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Twelve BALB/C nude male mice (age, 6–8 weeks; weight, 20 ± 2 g; Beijing Huafukang Biotechnology Co., Ltd., Beijing, China) were subcutaneously injected 200 μl of SGC7901 cell suspension (1 × 107/mL). When the mean tumor volume reached approximately 100–200 mm3, the tumor-bearing mice were randomized into two groups: SJZ group (13.2 g/kg/day SJZ decoction) and Model group (saline only). Six mice were included in each group. After 3-week treatment, mice were euthanized and tumors were immediately removed, rapidly frozen in liquid nitrogen, and stored at −80°C for subsequent analyses.
3
ASB16-AS1 Knockdown Inhibits Tumor Growth
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All animal studies were approved by the Committee on the Ethics of Animal Experiments of China-Japan Union Hospital Jilin University, and were conducted in compliance with the Animal Protection Law of the People’s Republic of China-2009 for experimental animals. We mentioned this information in the manuscript. The lentiviral plasmids (Hanbio Biotechnology Co., Ltd.) that specifically and stably expressed ASB16-AS1-targeting short hairpin RNA (sRNA; sh- ASB16-AS1) and target-free shRNA (sh-NC) were chemically produced by Hanbio Biotechnology Co., Ltd (Shanghai, China). U2OS cells were infected with the above lentiviral plasmids and then selected via Puromycin. U2OS cells stably expressing sh-ASB16-AS1 or sh-NC were subcutaneously injected into BALB/c male nude mice (Beijing HFK Bioscience Co., Ltd; Beijing, China). A vernier caliper was utilized to record the width and length of tumor xenografts. The tumor volume was determined with the following formula: volume = 1/2 × length × (width).2 (link) At the termination of the in vivo assay (4 weeks post-injection), all mice were sacrificed, and tumor xenografts were collected for further use.
4
Xenograft Tumor Model in BALB/c Mice
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BALB/c male nude mice were obtained from Beijing HFK Bioscience (Beijing, China). Cell suspensions (1 × 106 cells in 0.1 ml PBS) were subcutaneously injected into the dorsal right flank of mice (n = 5 per group) aged 4 weeks of age. The longest and shortest diameters of tumor masses were measured every 4 days for 28 days and then mice were killed. Tumor volume was calculated as follows: volume (mm3) = (shortest diameter)2 × (longest diameter) × 0.5 [43 (link)]. After tumor excision, tumor weight was recorded. Care of animals and all animal-related experiments were performed according to the institutional and national guidelines for animal experiments.
5
Xenograft Tumor Model in Nude Mice
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Male BALB/c- nude mice (weighing approximately 19 g; aged 4 weeks) were purchased from Beijing Huafukang Bioscience and maintained in pathogen-free conditions. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Huazhong University of Science and Technology. Guidelines for Experimental Animal Ethical Committee of Huazhong University of Science and Technology and Experimental animals administrative regulations of Hubei Province were followed for the welfare of the animals. Each treatment group consisted of 3 random mice who were housed in the animal centre of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China). We resuspended MHCC-97H cells and L-02 cells alone or together with PTFs in serum-free medium, combining together with matrigel in a 1:1 ratio and injected them subcutaneously into flanks of the nude mice. The length (L) and width (W) of the tumors were measured externally every 2 days as from the seventh day. Tumor volume was calculated with the formula: V=(L×W2)/2. After 14 days, all mice were sacrificed, and the tumors were excised and measured. AFP Antibody (cat#14550-1, proteintech) was used for Immunohistochemical staining of the tumors at a dilution of 1:50.
6
Xenograft Tumor Growth Assay in Mice
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Male BALB/c nude mice (4–6 weeks) were purchased from Beijing HFK Bioscience Company (Beijing, China) and maintained under specific pathogen-free (SPF) condition. CWR22rv1 cells (106) with stably infection of shRNA-GABARAPL1 or control shRNA were suspended in 50 μL RPMI1640 and 50 μL Martigel were randomly injected s.c. into the left and right flank of mice (5 mice/group). Tumor volume (cubic millimeters) was measured weekly using a caliper, applying the formula [volume = 0.52 × (width)2 × (length)] for approximating the volume of a spheroid. When the experiment ends, mice were terminally narcotized and sacrificed. This study was performed in accordance with animal use protocols approved by the Committee for the Ethics of Animal Experiments, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center (SPHMC) (protocol number 2011-004). All animals were handled in accordance with the guidelines of the Committee for the Ethics of Animal Experiments, SPHMC.
7
Nude Mice Xenograft Model for Cancer Study
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Male BALB/c nude mice aged 3 weeks were obtained from Beijing HFK Bioscience Co., Ltd. in Beijing, China. After a 1‐week adaptation period, the mice that were maintained in a temperature and humidity‐controlled and specific pathogen‐free environment in the laboratory animal facility of Zhongnan Hospital of Wuhan University, were injected into the right flank with 3 × 106 T24 cells dispersed in 0.2 mL PBS. Ten days later, mice with transplanted tumours that were approximately 3 × 3 mm were randomly assigned to one of two groups (n = 5). GW9662 (1 mg/kg body weight) and vehicle were injected intraperitoneally every other day for 2 weeks. Tumour size was measured every 2 days using a caliper, and the values were calculated as follows: tumour volume (mm3) = length × width2 × 0.5 mm3. After the mice were killed, all the tumours tissues were dissected and fixed in 4% PFA preparing for subsequent H&E staining and IF staining.
8
Subcutaneous Tumor Xenograft Model
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Male BALB/c-nude mice (18–20 g) were purchased from HFK Bioscience Co. Ltd (Beijing, China). Each mouse was subcutaneously inoculated with 1 × 106 cells. The kinetics of tumor formation was assessed by measuring the tumor sizes with a digital caliper at 3-day intervals. Tumor volume was calculated using the following formula: volume = 0.5 × length × width2. Tumor weight was measured after separation from each mouse. All applicable international, national, and institutional guidelines for the care and use of animals were followed.
9
Evaluating PDIA6 in Pancreatic Cancer
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Healthy male BALB/c nude mice (16 ± 1 g) aged 6 weeks were purchased from Beijing Huafukang Biotechnology Co., Ltd. (Beijing, China). The details of housing and husbandry conditions were: 12:12 h light/dark cycle, 22 ± 1°C (temperature), and 45% to 55% (humidity). Mice were given free access to water and food. Six mice were kept in a cage. The cancer model was established after 1 week of adaptive feeding. Forty-eight mice were randomly divided into the following groups (n = 6 each group): shRNA-NC (injected with PANC-1 or AsPC-1 cells transfected with shRNA-NC), shRNA1-PDIA6 (injected with PANC-1 or AsPC-1 cells with stable knockdown of PDIA6), shRNA2-PDIA6 (injected with PANC-1 or AsPC-1 cells with stable knockdown of PDIA6), OV-NC (injected with BxPC-3 cells transfected with OV-NC), and OV-PDIA6 (injected with BxPC-3 cells with stable overexpression of PDIA6). shRNA-NC and OV-NC served as control groups. PC cells in the logarithmic growth phase (a total of 2 × 106 cells in 50 μl of Matrigel per cell type) were subcutaneously injected into the nude mice. Every 3 days, the 2 perpendicular diameters of tumors were detected, and the tumor volume was calculated. After 21 days, the mice were euthanized by intraperitoneal injection of sodium pentobarbital (200 mg/kg), and tumor tissues were collected for the following experiments.
10
Inhibition of DLX6-AS1 Suppresses Tumor Growth
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Male BALB/c nude mice (6 weeks old, n=6) were purchased from Beijing HFK Bioscience Co. Ltd. (Beijing, China) and were maintained under pathogen-free conditions with the approval of the Affiliated Hospital of Jining Medical University. For tumor propagation analysis, 1×107 A549 tumor cells, transfected with a short hairpin RNA (shRNA) that targeted DLX6-AS1 (shDLX6-AS1) or a negative control shRNA (shNC), were subcutaneously injected into BALB/c nude mice. Tumor volume was calculated at the indicated time points using the formula, volume = πab2 (link)/6 (a, tumor length; b, tumor width). Tumors were weighed 3 weeks after injection. Animal experiments were approved by the Animal Care and Use Committee of the Affiliated Hospital of Jining Medical University and were performed in accordance with the relevant guidelines and regulations of the committee. The levels of Ki67 in tumor were detected by immunohistochemical staining.
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