Identification of natural musk and artificial musk in CPZHs were performed on an Agilent 7890B gas chromatography coupling to Agilent 7000D triple quadrupole mass spectrometry (Agilent Technologies, Santa Clara, CA, USA). The collision cell gas, nitrogen flow was set at 1.5 ml/min and the quenching gas, helium flow at 2.25 ml/min. The column initial temperature was kept at 190 ℃. GC oven temperature was increased from 190 ℃ to 260 ℃ at a rate of 15 ℃/min and held for 5 min. The remaining chromatographic conditions are the same as the method described above. To develop an MRM method based on electron impact mass spectra, a unique precursor ion was selected followed at optimum collision energy (CE) to further fragment the precursor ion into product ions. The target ion of prasterone was m/z 288 and confirmative ions was m/z 270 with RT at 8.239 min at a CE of 10 eV. The target ion of androsteron was m/z 290 and confirmative ions was m/z 275 with RT at 8.296 min at a CE of 12 eV.
Agilent 7000d
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7000D is a gas chromatograph-mass spectrometer (GC-MS) system designed for chemical analysis. It provides high-performance separation and detection capabilities for a wide range of applications, including environmental, pharmaceutical, and food analysis. The Agilent 7000D combines a gas chromatograph with a triple quadrupole mass spectrometer, enabling precise identification and quantification of chemical compounds.
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Lab products found in correlation
Agilent 7890b, by Agilent Technologies (3 mentions)
Gc ms system, by Agilent Technologies (1 mentions)
Masshunter workstation software, by Agilent Technologies (1 mentions)
Agilent 7890a, by Agilent Technologies (1 mentions)
6470 lc tq, by Agilent Technologies (1 mentions)
Hp 5ms ui, by Agilent Technologies (1 mentions)
9 protocols using agilent 7000d
1
Identifying Natural and Artificial Musk
2
Profiling Trichoderma Bioactive Compounds
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The bioactive constituents within the cell-free supernatant of Trichoderma culture filtrate were elucidated and characterized through gas chromatography mass spectroscopy (GC–MS) analysis. For this purpose, the ethyl acetate extract was obtained as previously stated in “Effect of Trichoderma isolates against the radial growth of Alternaria pathogen in vitro ” section. The resultant residues were subjected to GC–MS analysis using an Agilent 7000D instrument (Santa Clara, CA, USA) and the program conditions were programed according to Khamis et al.66 (link).
Furthermore, the volatile organic compounds (VOCs) present in the Trichoderma sample were extracted using solid phase microextraction (SPME) at 40 °C for 20 min. The extracted VOCs were then injected into a GC–MS system (Agilent Technologies) equipped with a gas chromatograph model 7890B and a mass spectrometer detector model 5977A (Agilent technologies, Santa Clara, CA, USA)67 (link).
Furthermore, the volatile organic compounds (VOCs) present in the Trichoderma sample were extracted using solid phase microextraction (SPME) at 40 °C for 20 min. The extracted VOCs were then injected into a GC–MS system (Agilent Technologies) equipped with a gas chromatograph model 7890B and a mass spectrometer detector model 5977A (Agilent technologies, Santa Clara, CA, USA)67 (link).
3
Fatty Acid Profiling of Dinoroseobacter shibae
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For the fatty acid analysis, samples were prepared from approximately 60 mg D. shibae wet cell material or 10 mg D. shibae vesicle preparation according to the highly standardized Sherlock microbial identification system (MIS) (MIDI, Microbial ID, Newark, DE, USA). Samples were dried and resolved in 40 μl tert-butylmethylether (MTBE). Following the gas chromatography-flame ionization detection (GC-FID) analysis of the MIDI system, 1 μl of the sample was injected into an Agilent 7890B gas chromatograph equipped with an Agilent 7000D mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). The injector was set to 170°C and heated to 350°C at 200°C/min and held for 5 min. The GC run started at 170°C, and the program was as follows: 3°C/min to 200°C, 5°C/min to 270, and 120°C to 300°C, hold for 2 min. The MS source temperature was set to 230°C, the electron energy was set to 70 eV, and the mass range was scanned from 40 to 600 m/z. The samples from cell materials were analyzed splitless and additionally with a split of 7.5. Data were evaluated using the MassHunter Workstation software (version B.08.00; Agilent Technologies).
4
Headspace Wheat Seedlings Volatile Analysis
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An aliquot (1 μL) of headspace wheat seedlings volatile extract was analyzed by GC/MS on an Agilent 7890A coupled with Agilent 7000D (Agilent Technologies Co., Ltd, Santa Clara, CA, USA). The mass spectrometer was equipped with an Inert Cap 5MS/NP capillary column (5% diphenyl and 95% dimethylpolysiloxane, 30 m × 0.25 mm × 0.25 μm film thickness). Analysis was performed in the splitless mode using helium as carrier gas at a constant flow rate of 1 mL/min The oven temperature was maintained at 40 °C for 1 min, increased with a rate of 5 °C/min to 60 °C, held for 5 min. and then with a rate at 10 °C/min to 250 °C, held for 5 min, and spectra were recorded at 70 eV, source temperature 250 °C. Solvent delay was 5 min. Tentative identifications were made by comparison of spectra with mass spectral databases (NIST, Wiley). The relative content of volatiles was calculated by Agtqual software according to the relative peak area.
5
GC-MS Quantitative Analysis Protocol
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The quantitative analysis was performed on an Agilent 7890B gas chromatography system coupled with an Agilent 7000D triple quadrupole mass spectrometer (Agilent Technologies, USA). The processed samples were placed in the vials of the autosampler for injection. The chromatographic column was the DB-5UI capillary column (30 m × 0.25 mm, 0.25 µm). The separation procedure of the GC system was set at the initial temperature of 80 °C held for 1 min, then heated up to 300 °C at the speed of 10 °C/min and held for 5 min. No splitting mode was set. Helium was the carrier gas with a 1.0 mL/min flow rate. The temperature of interface, ion source, and Quadrupole temperature were set at 280 °C, 230 °C, and 150 °C, respectively.
6
GC-MS Analysis of Floral Volatiles in A. fimbriata
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To investigate the floral volatile production of A. fimbriata, we collected the newly opened flowers for gas chromatography–mass spectrometry (GC–MS) analysis, with the added 0.0825 μg of 3-octanol as an internal standard. Then, the samples were incubated at 40 °C for 30 min. The volatiles were further extracted using SPME fibre with 50/30 μm of divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) (Supelco Co.). Finally, GC–MS analysis was conducted on an Agilent 7890B gas chromatograph coupled to a mass spectrometer (Agilent 7000D) with a fused silica capillary column (HP-5MS) coated with polydimethylsiloxane (19091S-433UI) (30 m × 0.25 mm internal diameter, 0.25 μm film thickness). The oven temperature was programmed to start at 40 °C for 3 min and then ramped to 130 °C at a rate of 5 °C min–1, followed by a second ramp to 156 °C at a rate of 2 °C min–1 and the final ramp to 280 °C at a rate of 10 °C min–1. Three biological replicates were conducted for the GC–MC analysis.
7
Quantitative Analysis of Compounds
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Analytical balance, vortex, benchtop centrifuge, 50 mL centrifuge tubes, sonicator, adjustable pipettes: 10–100, 20–200, and 100–1000 µL with disposable tips, 10 mL volumetric flasks, LCMSMS: Agilent 6470-LC/TQ, GCMSMS: Agilent 7000D, Analytical Column: Poroshell EC-C18 2.7 µm (2.7 µm, 3.0 × 150 mm), HP-5MS UI 15 m, 0.25 µm, 25 mm ID Capillary column, mobile phase filtration assembly were used for the study.
8
Quantitative Analysis of Pesticide Residues by GC-MS/MS
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The content analysis of pesticide residues was performed using a GC-MS/MS system Agilent 7000D (Agilent Technologies, USA). A HP-5MA Ultra Inert capillary chromatographic column (15 m × 0.25 mm × 0.25 μm) was utilized for the GC separations. The temperature of the column was as follows: 40°C for 1 min, 40°C/min to 120°C, 5°C/min to 240°C, and 12°C/min to 300°C for 6 min. Nitrogen (purity ≥ 99.999%) was used as the carrier gas with the flow rate of 1.0 mL/min. The injection port temperature and transfer-line temperature were 280 °C. The injection volume in unshunted mode was 1 μL. The analysis in mass spectrometer was under MRM mode and the electron impact ion source was at 70 eV. The ion source temperature was maintained at 280 °C. The solvent delay was for 4 min.
9
GC-MS Analysis of Trimethylsilyl Melamine
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Analyses were performed by gas chromatograph Agilent 7890B (Agilent Technologies, USA) with column HP-5MS-UI (5% phenyldimethylpolysiloxan, 30 m × 0.25 mm × 0.25 µm). The sample injection of 2 µl was made in a split mode (3:1) at 250 °C, with a constant flow of helium of 1 mL/min (6.0 purity, Air Products, Czech Republic). Septum purge was set to 3 mL/min and a gradient temperature program was 75 °C (1 min) -10 °C/min -220 °C (2 min) -10 °C/min -260 °C (0 min) -25 °C/min -300 °C (2 min). Trimethylsilyl derivative of melamine was detected by the triple quadrupole mass spectrometer Agilent 7000D (Agilent Technologies, USA), with interface temperature set to 250 °C. Electron ionization was carried out at 70 eV at 230 °C, and collision energy of nitrogen gas was set at 10 eV (6.0 purity, Air Products, Czech Republic). Detection in the product ion mode of the parent ion m/z 327 was adapted. Optimization, validation parameters and quantification results were calculated from the peak area of the quantification ion m/z 171.
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