Differential interference microscope

Manufactured by Zeiss

Sourced in Germany

The Differential-interference microscope is an optical microscope that uses the interference of polarized light to enhance the contrast in transparent or semi-transparent samples. It allows for the visualization of detailed structures within the sample by detecting minute differences in optical path lengths.

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Lab products found in correlation

Orca flash 4, by Hamamatsu Photonics (2 mentions)

Close spelling variants of this product

Differential interference contrast microscope (3 citations)

3 protocols using differential interference microscope

Real-time Analysis of Eh-rPrx Effects

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RAW264.7 cells were collected in the logarithmic growth phase and placed in 4-chamber glass bottom 35-mm dishes (In Vitro Scientific, Sunnyvale, CA, USA, D35C4-20-1-N) with 2.5 × 10⁵ cells/chamber. After the cells adhered and fully stretched, serum-free medium containing Eh-rPrx was added and real-time changes in cell morphology were immediately observed under a differential interference microscope (Carl Zeiss Meditec, Jena, Germany).

Li X., Zhang Y., Zhao Y., Qiao K., Feng M., Zhou H., Tachibana H, & Cheng X. (2020). Autophagy Activated by Peroxiredoxin of Entamoeba histolytica. Cells, 9(11), 2462.

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Microscopic Analysis of Ciliary Beating

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The warmed up samples were evaluated under a differential-interference microscope (Zeiss, Oberkochen, Germany) at × 1000 magnification and cilia beat was recorded with a digital high-speed video (DHSV) camera (Hamamatsu Orca Flash 4.0 Hamamatsu, Japan) with a frame rate of 400 Hz. DHSV video sequences were played back frame by frame and cilia beat frequency (CBF) was determined by calculating the mean of all recorded cilia beat cycles and the cilia beating pattern (CBP) was determined by two independent expert operators. DHSV was repeated on two different occasions.

Schultz R., Elenius V., Lukkarinen H, & Saarela T. (2020). Two novel mutations in the DNAH11 gene in primary ciliary dyskinesia (CILD7) with considerable variety in the clinical and beating cilia phenotype. BMC Medical Genetics, 21, 237.

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High-Speed Video Microscopy of Nasal Cilia

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Nasal epithelial cells were suspended in DMEM medium and evaluated under a differential-interference microscope (Zeiss, Oberkochen, Germany) at ×1,000 magnification and cilia beat was recorded with a digital high-speed video (DHSV) camera (Hamamatsu Orca Flash 4.0, Hamamatsu Japan) with a frame rate of 256 Hz. The detailed protocol for HSVM can be found in Schultz et al., 2022 (link). DHSV video sequences were played back frame by frame and cilia beat frequency (CBF) was determined by calculating the mean of all recorded cilia beat cycles and the cilia beating pattern (CBP) was determined by two independent expert operators. HSVM was repeated on two different occasions.

Schultz R., Elenius V., Fassad M.R., Freke G., Rogers A., Shoemark A., Koistinen T., Mohamed M.A., Lim J.S., Mitchison H.M, & Sironen A.I. (2022). CFAP300 mutation causing primary ciliary dyskinesia in Finland. Frontiers in Genetics, 13, 985227.

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