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5 5 ditiobis 2 nitrobenzoic acid dtnb

Manufactured by Merck Group
Sourced in Germany

5,5-ditiobis(2-nitrobenzoic) acid (DTNB) is a chemical compound used as a reagent in various laboratory applications. It is a yellow crystalline solid with the chemical formula C₁₄H₈N₂O₆S₂. DTNB is commonly used for the detection and quantification of thiol groups (-SH) in proteins and other biomolecules.

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2 protocols using 5 5 ditiobis 2 nitrobenzoic acid dtnb

1

Synthesis and Characterization of Bioactive Compounds

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Anisaldehyde, 3,5-dichloroaniline, and monohydrated hydrazine were purchased from Merck (Darmstadt, Germany), phenol, ascorbic acid, urease (from jack bean), DPPH (2,2-diphenyl-1-picrylhydrazyl), acetylthiocholine iodide (ATCI), acetylcholinesterase, and 5,5-ditiobis(2-nitrobenzoic) acid (DTNB) were from Sigma-Aldrich (Steinheim, Germany). Sodium hypochlorite, and sodium salicylate were from Daejung (Siheung City, Korea). Lithium chloride, sodium nitrite and sodium nitroprusside were from Riedel de Haën (Seelze, Germany). Urea, ethylenediaminetetraacetic acid and thiourea were from BDH Labs (Cambridge, England).
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2

Protein Expression and Purification

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Uric acids sodium salt (U2875) and 5,5′-ditio-bis 2-nitrobenzoic acid (DTNB) were obtained from Sigma-Aldrich. Restriction enzymes,
T4 DNA ligase, and isopropyl-b-D-thiogalactopyranoside (IPTG) was obtained from Fermentas. Plasmid extraction kit, gel purification kit,
were obtained from Bioneer Corp. Ni2+-NTA agarose (30210) was from Qiagen. Bacterial culture medium was from Scharlau company.
SDS- PAGE kit and protein assay kit were from Arsam Fara Zist Corp., Iran.
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