Chromium single cell 3 reagent kit v2 v3

Manufactured by 10x Genomics

The Chromium Single Cell 3' Reagent kit v2/v3.1 is a laboratory equipment product from 10x Genomics designed for single-cell analysis. The kit contains the necessary reagents for partitioning individual cells into nanoliter-scale gel beads and performing downstream processing and library preparation for next-generation sequencing.

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Lab products found in correlation

Qubit 2.0 fluorometer, by Thermo Fisher Scientific (4 mentions) Kapa hifi ready mix, by Roche (2 mentions) Small rna rpix truseq d7xx, by Illumina (2 mentions) Fragment analyzer, by Agilent Technologies (2 mentions) Novaseq 6000, by Illumina (2 mentions) Nextseq 500, by Illumina (2 mentions)

2 protocols using chromium single cell 3 reagent kit v2 v3

Single-Cell Multimodal Sequencing Protocol

Cited 1 time

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scRNA-seq libraries were prepared using the Chromium Single Cell 3′ Reagent kit v2/v3.1 chemistry (10x Genomics) following the manufacturer’s instructions with the modifications required for recovery of ADTs and HTOs^{19 (link)}. ADT and HTO libraries were amplified using the KAPA HiFi ready mix (Roche) with sample-specific index primers (Illumina small RNA RPIx/Truseq D7xx)^{19 (link)} and purified using SPRIselect reagent. Library size and quality were analyzed using a Fragment Analyzer (Advanced Analytical) before fragmentation and after final purification. Final library concentrations were measured on a Qubit 2.0 fluorometer (Thermo Fisher). Libraries were sequenced on a NextSeq500 or NovaSeq6000 sequencer (Illumina) using the read configurations recommended by 10x Genomics (v2: R1 26 cycles, R2 98 cycles, I1 8 cycles; v3: R1 28 cycles, R2 91 cycles, I1 8 cycles).

Rückert T., Lareau C.A., Mashreghi M.F., Ludwig L.S, & Romagnani C. (2022). Clonal expansion and epigenetic inheritance of long-lasting NK cell memory. Nature Immunology, 23(11), 1551-1563.

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Multiplexed scRNA-seq Library Preparation

Cited 1 time

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scRNA-seq libraries were prepared using the Chromium Single Cell 3’ Reagent kit v2/v3.1 chemistry (10x Genomics) following the manufacturer’s instructions with the modifications required for recovery of ADTs and HTOs^{19 (link)}. ADT and HTO libraries were amplified using the KAPA HiFi ready mix (Roche) with sample-specific index primers (Illumina small RNA RPIx/Truseq D7xx)^{19 (link)} and purified using SPRIselect reagent. Library size and quality were analyzed using a Fragment Analyzer (Advanced Analytical) before fragmentation and after final purification. Final library concentrations were measured on a Qubit 2.0 fluorometer (Thermo Fisher). Libraries were sequenced on a NextSeq500 or NovaSeq6000 sequencer (Illumina) using the read configurations recommended by 10x Genomics (v2: R1 26 cycles, R2 98 cycles, I1 8 cycles; v3: R1 28 cycles, R2 91 cycles, I1 8 cycles).

Rückert T., Lareau C.A., Mashreghi M.F., Ludwig L.S, & Romagnani C. (2022). Clonal expansion and epigenetic inheritance of long-lasting NK cell memory. Nature Immunology, 23(11), 1551-1563.

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