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Erbb2

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Erbb2 is a lab equipment product used for research purposes. It is a receptor tyrosine kinase that plays a role in the regulation of cell growth and differentiation. The core function of Erbb2 is to facilitate the study of signaling pathways and cellular processes related to this protein.

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Lab products found in correlation

Phospho erk, by Cell Signaling Technology (2 mentions) Phospho akt, by Cell Signaling Technology (2 mentions) Erk1 2, by Cell Signaling Technology (2 mentions) Egfrl858r, by Cell Signaling Technology (1 mentions) Phospho egfr y1068, by Cell Signaling Technology (1 mentions) Anti rabbit hrp antibody, by Cell Signaling Technology (1 mentions) Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Phospho s6, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Supersignal west pico, by Thermo Fisher Scientific (1 mentions) Femto chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Phospho erbb2, by Cell Signaling Technology (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Phospho egfr, by Cell Signaling Technology (1 mentions) Imagequant las 4000, by GE Healthcare (1 mentions) Cox 4, by Cell Signaling Technology (1 mentions) Hsp60, by Abcam (1 mentions) Caspase 8, by Cell Signaling Technology (1 mentions) Cd133, by Santa Cruz Biotechnology (1 mentions)

4 protocols using erbb2

1

Western Blot Analysis of Signaling Proteins

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Crushed tumors were lysed in ice-cold RIPA lysis buffer [50 mM Tris, pH 8.0, 150 mM NaCl, 5 mM MgCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, protease and phosphatase inhibitor cocktail (Thermo Scientific)]. Equal amounts of total protein were separated by SDS-PAGE and probed as indicated. Signals were detected using either SuperSignal West Pico or Femto chemiluminescent substrates (Pierce Biotechnology). Antibodies for immunoblotting against EGFRL858R (#3197), phospho-EGFR-Y1068 (#2234), phospho-Erbb2-Y1248 (#2247), AKT (#2938), phospho-AKT (#4060), ERK1/2 (#9102), phospho-ERK (#4376), S6 (#2217), phospho-S6 (#5364), GAPDH (#2118) and the secondary anti-rabbit HRP antibody (#7074) were from Cell Signaling Technology (CST). Additional antibodies include: Erbb2 (Millipore, 06-562), and SPC (Abcam, #ab90716). All the antibodies were used at the dilutions suggested by manufacturer.
Pirazzoli V., Ayeni D., Meador C.B., Sanganahalli B.G., Hyder F., de Stanchina E., Goldberg S., Pao W, & Politi K. (2015). Afatinib plus cetuximab delays resistance compared to single agent erlotinib or afatinib in mouse models of TKI-naïve EGFR L858R-induced lung adenocarcinoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(2), 426-435.
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2

Quantitative Protein Analysis by Western Blot

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Protein abundance and phosphorylation levels were quantified by Western blot
assay. Antibodies were obtained as follows; antibodies for phospho-EGFR (Y1068),
phospho-ErbB2 (Y1221/1222), Akt, phospho-Akt (T308), ERK, phospho-ERK
(T202/Y204), p38, phospho-p38 (T180/Y182), phospho-ESR (S167), JNK , phospho-JNK
(T183/Y185), FOS, phospho-FOS (S32), phospho-JUN (S63), phospho-SRF, and SRF
from Cell Signaling Technology; ESR and phospho-ESR (S118) from Upstate
technology; EGFR from Fitzgerald, ErbB2 from Millipore; Antibodies for FRA1
(FOSl1), RARA, and DUSP5 from Santa Cruz Biotechnology. Time-course samples of
EGF and HRG were run on different gels and then normalized by the relative ratio
given from the blots on the same membrane (Supplementary Figures S1-S2). Two biological replicates were used for
each time-point in each time-course. Protein bands were detected with
chemi-luminescence and quantification was performed using Image Quant LAS 4000
(GE Healthcare).
Mina M., Magi S., Jurman G., Itoh M., Kawaji H., Lassmann T., Arner E., Forrest A.R., Carninci P., Hayashizaki Y., Daub C.O., Okada-Hatakeyama M, & Furlanello C. (2015). Promoter-level expression clustering identifies time development of transcriptional regulatory cascades initiated by ErbB receptors in breast cancer cells. Scientific Reports, 5, 11999.
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3

Comprehensive Protein Expression Analysis

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Cells and homogenised tumour tissue were lysed, and total protein (30 μg) resolved by SDS-PAGE and transferred to PVDF membranes, which were probed with following antibodies: EpCAM, erbB2 (both from Sigma-Aldrich), caspase-9, caspase-8, cleaved caspase-3, VDAC, COX IV, SDHA (all from Cell Signaling), CD44, HSP60, actin (all from Abcam), CD133, PARP-1/2 (both from Santa Cruz), and SDHC (Novus Biologicals). ECL western blotting substrate (Thermo Scientific) and ChemiDoc™ XRS+ System (BioRad) were used to visualise and evaluate the blots.
Yan B., Stantic M., Zobalova R., Bezawork-Geleta A., Stapelberg M., Stursa J., Prokopova K., Dong L, & Neuzil J. (2015). Mitochondrially targeted vitamin E succinate efficiently kills breast tumour-initiating cells in a complex II-dependent manner. BMC Cancer, 15, 401.
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4

Protein Analysis of Tumor Extracts

Cited 1 time
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Tumors were isolated from euthanized, VIA-treated mice and immediately flash frozen. Tissue extracts were prepared as previously described [27 (link)]. Equal amounts of proteins (50 μg) were resolved by 4–15% gradient SDS PAGE. After transfer, membranes were probed with specific antibodies recognizing target proteins: pTyr (Sigma), ErbB2, ErbB3, Akt, pAkt473, Erk 1/2, pErk1/2 (Cell Signaling, Beverly, MA, USA), survivin, actin (Sigma, St. Louis, MO, USA), 4EBP-1, p4EBP-1, s6, ps6 (Santa Cruz Biotech, Santa Cruz, CA, USA), and then with IRDye 800 conjugated anti-rabbit or mouse IgG or Alexa Fluor 680 anti-rabbit IgG, and were visualized using the Odyssey Infrared Imaging System (LI-COR, Lincoln, NE, USA) as previously described [27 (link)].
Osada T., Hartman Z.C., Wei J., Lei G., Hobeika A.C., Gwin W.R., Diniz M.A., Spector N., Clay T.M., Chen W., Morse M.A, & Lyerly H.K. (2018). Polyfunctional anti-human epidermal growth factor receptor 3 (anti-HER3) antibodies induced by HER3 vaccines have multiple mechanisms of antitumor activity against therapy resistant and triple negative breast cancers. Breast Cancer Research : BCR, 20, 90.
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