Mcf 10a

The human breast cancer cell lines BT474, SKBR3, MDA-MB-453 (American Type Culture Collection, ATCC), JIMT-1 (DSMZ GmbH, Germany), and MDA-MB-231 (PerkinElmer, Inc., CT) were cultured in DMEM, RPMI 1640 or MEM (Gibco, MD) containing 10% fetal bovine serum (FBS) and penicillin-streptomycin (100 U/mL). The normal human mammary epithelial cell line MCF10A (ATCC) was cultured in Mammary Epithelial Cell Growth Medium (MEGM), including hEGF, insulin, hydrocortisone, and bovine pituitary extract (SingleQuots^TM Kit, Lonza, CA) with streptomycin-penicillin (100 U/mL). Cells were incubated at 37 °C in an atmosphere of 5% CO₂.

Human MM cell lines MSTO-211H (MSTO parent) and NCI-H226 were obtained from the American Type Culture Collection (ATCC, Rockville, MD). MSTO parent cells were stably transfected with a full-length human CD26 (MSTO-CD26) [6 (link)]. Human MM cell line JMN cells were transduced with the short hairpin RNA (shRNA)-expressing lentivirus, generating the stable cell lines JMN CD26-shRNA and JMN ctrl-shRNA [19 (link)]. For non-tumor human cells, immortalized pleural mesothelial cell line MeT-5A, mammary epithelial cell line MCF10A, fetal lung fibroblast cell line TIG-1, human umbilical vein endothelial cells (HUVEC), and human dermal microvascular endothelial cells (HDMVEC) were used. MeT-5A and MCF10A were obtained from ATCC. TIG-1 was obtained from JCRB Cell Bank (Osaka, Japan). HUVEC, HDMVEC and the culture media for MCF10A, HUVEC, HDMVEC (MEGM, EGM-2, EGM-2MV, respectively) were purchased from LONZA (Walkersville, MD). MSTO parent, MSTO-CD26, JMN ctrl-shRNA, JMN CD26-shRNA, H226 and MeT-5A were grown in RPMI 1640 medium supplemented with 10% FBS. TIG-1 was grown in DMEM medium supplemented with 10% FBS. All the cells were cultured at 37 °C in a humidified 5% CO₂ incubator.

The human breast cancer cell lines, MDA-MB-231, Hs578T, SKBR3, BT-20, MDA-MB-468, MCF-7, and T47D were purchased from ATCC (American Type Culture Collection) and cultured in Dulbecco's modified Eagle's medium (DMEM) (Lonza) supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin antibiotics. Immortalized MCF-10A cells were cultured in MEGM medium (CC-3150, Lonza) containing 100 ng/ml of cholera toxin to make a complete growth culture medium. All cell lines were grown in a 37°C humidified incubator with 5% CO₂.