Aca2000 340km

We used the same experimental imaging setup as that in a previous study.³⁰ (link) Low-coherence light with a central wavelength of 540 nm, bandwidth of 10 nm, and power of 100 mW was used to illuminate the sample. Validatory experiments were conducted using 2.5-day-old chicken embryos, which served as live biological samples. Raw blood flow images with a resolution of $256\times 256\text{\hspace{0.17em}\hspace{0.17em}pixels}$ were captured using high-performance telecentric lenses (magnification: $1.7\times$ , #63-232, Edmund Optics) and a high-speed complementary metal oxide semiconductor camera (acA2000-340 km, Basler, Germany). The exposure time and sampling rate of the camera were set to $100\mu \mathrm{s}$ and 500 fps, respectively. The camera had a pixel size of $5.5\mu \mathrm{m}\times 5.5\mu \mathrm{m}$ , and thus, the imaged area was $0.83\mathrm{mm}\times 0.83\mathrm{mm}$ . Six datasets with 1024-frame raw blood flow images were employed for the data preparation to obtain the training data. All components of the imaging setup were fixed on a vibration-isolation optical platform. During the experiments, the biological samples were handled carefully in accordance with the laboratory animal protocol approved by the Institutional Animal Care and Use Committee of Foshan University.