Control shrna plasmid a

Manufactured by Santa Cruz Biotechnology

Sourced in Israel

Control shRNA Plasmid-A is a plasmid designed for use as a negative control in RNA interference (RNAi) experiments. The plasmid contains a non-targeting shRNA sequence that does not have significant sequence homology to any known genes.

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Lab products found in correlation

Transfection reagent, by Santa Cruz Biotechnology (1 mentions) Ripa lysis and extraction buffer, by Thermo Fisher Scientific (1 mentions) Nci h460, by National Collection of Authenticated Cell Cultures (1 mentions) Nci h1975, by National Collection of Authenticated Cell Cultures (1 mentions) Nvp aew541, by Selleck Chemicals (1 mentions) Gefitinib, by Cell Signaling Technology (1 mentions) Cytoselect cell invasion assay kit, by Cell Biolabs (1 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions) Annexin 5 fitc apoptosis detection kit, by Abcam (1 mentions) Pepstatin a, by Merck Group (1 mentions)

Spelling variants of this product

Control shRNA (41 citations) Control shRNA plasmid (16 citations) Control-plasmid (14 citations) ShRNA plasmids (4 citations) ShRNA Plasmid-A (3 citations) Sc-108060 control shRNA plasmid-A (2 citations)

5 protocols using control shrna plasmid a

TauT and TonEBP knockdown in HCMs

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HCMs were transfected with either TauT shRNA(h) plasmid (catalog no. sc-61648-SH, Santa Cruz, CA, USA) to block the expression of TauT or with TonEBP shRNA(h) plasmid (catalog no. sc-43968-SH, Santa Cruz) to block the expression of TonEBP using HilyMax Transfection Reagent according to the manufacturer's protocol. Control shRNA Plasmid-A (catalog no. sc-108060, Santa Cruz) was used as a negative control.

Yang Y.J., Han Y.Y., Chen K., Zhang Y., Liu X., Li S., Wang K.Q., Ge J.B., Liu W, & Zuo J. (2015). TonEBP modulates the protective effect of taurine in ischemia-induced cytotoxicity in cardiomyocytes. Cell Death & Disease, 6(12), e2025-.

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Integrin Knockdown and Fluorescent Protein Tools

Cited 1 time

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Integrin β1 shRNA plasmid (#sc-29375-SH), Integrin α3 shRNA plasmid (#sc-35684-SH), Integrin α6 shRNA plasmid (#sc-43129-SH), and control shRNA plasmid-A (#sc-108060) were purchased from Santa Cruz Biotechnology for knockdown of the target integrins. The knockdown efficiency was evaluated by western blotting.
The mGFP-paxillin expression vector was a gift from A. Kusumi (Okinawa Institute of Science and Technology, Japan). pGFP-FAK and pEGFP-vinculin were gifts from Kenneth Yamada (RRID: Addgene_50513 and Addgene¬_50515)³⁹, EGFP-talin, mVenus-Integrin-Beta1 and mRuby-Lifeact-7 was a gift from Michael Davidson (RRID: Addgene_54560). pEGFPC1/Gg Vinculin 1-258 (akaVD1) was a gift from S. Craig (RRID: Addgene_46270)^{40 (link)}. The Rac1 FRET Sensor, pCAGGS-RaichuEV-Rac1was generously provided by K. Aoki (National Institute for Basic Biology, Japan)^{41 (link)} and the RhoA FRET sensor, DORA-RhoA was generously provided by Yi I. Wu (University of Connecticut Health Center, USA)⁴². The expression vectors for Rac1/Tiam1 activation system (Lyn11-linker-FRB, YFP-FKBP, YFP-FKBP-linker-Tiam1, and pTriEx-PA-Rac1) was generously provided by T. Inoue (Johns Hopkins University, USA)^{43 (link)}.

Hu X., Roy S.R., Jin C., Li G., Zhang Q., Asano N., Asahina S., Kajiwara T., Takahara A., Feng B., Aoki K., Xu C, & Zhang Y. (2022). Control cell migration by engineering integrin ligand assembly. Nature Communications, 13, 5002.

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Investigating EGFR and IGF-1R Inhibitors in Lung Cancer Cell Lines

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The cell lines NCI-H460 and NCI-H1975 were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were grown in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), and incubated at 37°C, 5% CO₂. The EGFR inhibitor Gefitinib (#4765) was purchased from Cell Signaling Technology. The IGF-1R inhibitor NVP-AEW541 (#S1034) was purchased from Selleckchem. RIPA lysis and extraction buffer (#89900) used to lyse cells was purchased from ThermoFisher. PIK3R2-shRNA (#sc-39125-SH) and the Control shRNA Plasmid-A (#sc-108060) were purchased from Santa Cruz Biotechnology. MiR-30a-5p mimics (5′-UGUAAACAUCCUCGACUGGAAG-3′) and the negative control RNA oligo (5′-UUCUCCGAACGUGUCACGUTT-3′) were purchased from GenePharma. PIK3R2-shRNA and miR-30a-5p mimics were transfected by lipofectamine 2000 reagent (#12566014) purchased from ThermoFisher. Annexin V-FITC Apoptosis Detection Kit (#K101-25) was purchased from BioVision. The CytoSelect™ Cell Invasion Assay Kit (#CBA-110) was purchased from CELL BIOLABS, INC.

Meng F., Wang F., Wang L., Wong S.C., Cho W.C, & Chan L.W. (2016). MiR-30a-5p Overexpression May Overcome EGFR-Inhibitor Resistance through Regulating PI3K/AKT Signaling Pathway in Non-small Cell Lung Cancer Cell Lines. Frontiers in Genetics, 7, 197.

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Autophagy Modulation Protocols

Cited 1 time

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DNAJC13 shRNA plasmid(sc-77968-SH) and Control shRNA Plasmid-A(sc-108060) were from Santa Cruz Biotechnology. GFP-LC3B, GFP-LAMP1, and mRFP-LC3 constructs were prepared as previously described[70 (link)–76 (link)]). E64D (Cat. 330005), and Pepstatin A (Cat.516481) (Sigma).

Swords S., Jia N., Norris A., Modi J., Cai Q, & Grant B.D. (2023). A Conserved Requirement for RME-8/DNAJC13 in Neuronal Autolysosome Reformation. bioRxiv.

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Lentiviral Knockdown of SDF-1 in MEFs

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For stable knockdown of SDF-1 in MEFs, lentiviral shHIF1A purchased from MISSION shRNA specific for SDF-1 was used (TRCN0000184347, Sigma-Aldrich, Israel), while the Control shRNA Plasmid-A (SC-108060, Santa Cruz Biotechnology, Inc., Dallas, Texas) was used as control. HEK-293T cells were co-transfected with shSDF-1 or shControl-A plasmids. Lentiviruses-containing medium from HEK-293T transfected cells was used directly to infect MEFs. Infected cells were selected with puromycin (2 µg/ml) for two weeks.

Avraham S., Korin B., Aviram S., Shechter D., Shaked Y, & Aronheim A. (2019). ATF3 and JDP2 deficiency in cancer associated fibroblasts promotes tumor growth via SDF-1 transcription. Oncogene, 38(20), 3812-3823.

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