Exosome samples purified from U87 were incubated on freshly cleaved mica for 5 min, washed with deionized water to remove any unbound exosomes and air-dried overnight. Samples were imaged by Dimension ICON (Bruker Instruments, CA, USA) using the tapping mode via TESP cantilever (Bruker Instruments, CA, USA) and images were recorded at 1024 samples per line at 1 Hz. Image processing was done using SPIP™ software. For exosome yield calculation, images at a size of 1 μm × 1 μm and a resolution of 512 samples per line, at 1 Hz, were used. For exosome surface roughness measurements, images were processed with SPIP™ to obtain Ra (arithmetic average of the absolute values) and Rq (root mean squared) values.
Tesp cantilever
Manufactured by Bruker
Sourced in United States
The TESP cantilever is a lab equipment product designed for use in atomic force microscopy (AFM) applications. It functions as a probe to measure and analyze surface topography and properties at the nanoscale level.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tesp cantilever
1
Exosome Characterization by Atomic Force Microscopy
2
Barnacle Adhesion Analysis by AFM
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AFM images on base plate samples of barnacles adhered to Au-coated glass were acquired of the peripheral regions, in air, using intermittent contact mode imaging conditions with a Nanoman with Dimension V controller and TESP cantilever (Bruker). Image analysis was performed with WSxM software (Horcas et al. 2007 (link)).
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