Anti α tubulin and anti flag m2 antibodies

Anti-LC3B, anti-Bcl-xL, anti-Atg16L1, anti-Rac1, anti-Rho1, anti-Cdc42, anti-phospho-ERK, anti-EEA1 and anti-LAMP1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal antibodies reactive with LRP1 (11H4) were a kind gift from Dr. Strickland, University of Maryland School of Medicine, Baltimore. Fibronectin (EP5) and ubiquitin (P4D1) were from Santa Cruz Biotechnologies (Santa Cruz, CA, USA); anti-Cx43, anti-ERK, anti-Bcl-2 and anti-RPTPβ antibodies were from BD Biosciences (Tokyo, Japan); anti-multi ubiquitin monoclonal antibody (FK1) was from MBL (Nagoya, Japan); anti-GAPDH antibody was from GeneTex (Irvine, CA, USA) and anti-LC3 (clone 1703) antibody was from Cosmo Bio (Tokyo, Japan). Anti-RPTPα rabbit polyclonal antibodies for immunoblotting were provided by Dr. Jan Sap; anti-α-tubulin and anti-FLAG M2 antibodies, N-acetyl-l-cysteine (NAC) and ammonium chloride were from Sigma Aldrich (St. Louis, MO, USA); ProLong Gold Antifade Reagent with DAPI and DIDS were from Invitrogen (Eugene, OR, USA); an ERK inhibitor, U0126, was from Cayman Chemical (Ann Arbor, MI, USA); Rac1 inhibitor, NSC23766 was from Wako Pure Chemical Industries (Osaka, Japan); and GSH was from Merck (Darmstadt, Germany).

The following polyclonal and monoclonal antibodies were purchased: anti-USP19 antibody (A301-587A; Bethyl Laboratories, Montgomery, TX, USA); anti-HRD1 antibody (Novus Biologicals, Littleton, CO, USA); anti-α-tubulin and anti-FLAG M2 antibodies (Sigma-Aldrich); anti-c-Myc ant-HA antibodies (Roche, Indianapolis, IN, USA); anti-neomycin phosphotransferase II antibody (NPT II; clone AC113; Merck Millipore, Billerica, MA, USA); anti-K48-linked ubiquitin antibodies (clone Apu2; Merck Millipore); anti-syntaxin 6 antibody (BD Transduction Laboratories, San Jose, CA, USA) and anti-MHC class I antibody (clone EMR8-5; Hokudo, Sapporo, Japan). The anti-USP19 peptide antibody (#1) was made in a rabbit against the synthetic peptides KIDSSNREQRLED and VFYPLVSQSRWR (Operon Biotechnology, Tokyo, Japan) and was used in immunofluorescence staining (Figure S1).