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Cd3 h7apc sk7

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CD3 H7APC (SK7) is a fluorescently labeled antibody that binds to the CD3 antigen on human T cells. It is a useful tool for the identification and enumeration of T cells in flow cytometry analysis.

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Lab products found in correlation

Cd45ro trpe, by Beckman Coulter (2 mentions) Ctla 4 apc, by Beckman Coulter (2 mentions) Igd pe, by Beckman Coulter (2 mentions) Il 21 alexa 647, by Beckman Coulter (2 mentions) Cd127 cy5pe, by BioLegend (2 mentions) Ccr6 bv785, by Thermo Fisher Scientific (2 mentions) Cd27 bv605, by Thermo Fisher Scientific (2 mentions) Ccr7 bv605, by Thermo Fisher Scientific (2 mentions) Icos pac blue, by Thermo Fisher Scientific (2 mentions) Cd38 bv785, by Thermo Fisher Scientific (2 mentions) Cd20 apc h7, by Beckman Coulter (2 mentions) Igm pe cy5, by Beckman Coulter (2 mentions) Ki 67 alexa700, by Beckman Coulter (2 mentions) Ifng bv421 4, by BioLegend (2 mentions) Tnf α cy7pe, by Thermo Fisher Scientific (2 mentions) Il 2 alexa 700, by Thermo Fisher Scientific (2 mentions) Cd27 alexa594, by Thermo Fisher Scientific (2 mentions) Cd8 bv785, by Thermo Fisher Scientific (2 mentions) Cd19 pac blue, by Thermo Fisher Scientific (2 mentions) Cd154 pe, by Thermo Fisher Scientific (2 mentions)

3 protocols using cd3 h7apc sk7

1

Flow Cytometry Immunophenotyping Panel

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Flow cytometry was performed using the following directly conjugated antibodies: (1) BD Biosciences: CD3 H7APC (SK7), CD45RA Cy7PE (L48), CD20 APC-H7 (2H7), CD45RO TRPE (UCHL), CTLA-4 APC (BNI3), IgD PE (IA6-2), IgG APC (G18-145), IgM PE-Cy5 (G20-127), IL-21 Alexa 647 (3A3-N2.1), and Ki-67 Alexa700 (150503); (2) Beckman Coulter: CD127 Cy5PE (R34.34); (3) BioLegend: PD-1 BV711 (EH12.2H7), IFNg BV421 (4S.B3), TNF-α CY7PE (MAb-11), IL-2 Alexa-700 (MQ1-17H12), CCR6 BV785 (G034E3), CD27 Alexa594 (IA4CD27), CD27 BV605 (O323), CCR7-BV605 (G043H7), CD8 BV785 (SK1), ICOS Pac Blue (C398.4A), CD19 Pac Blue (HIB19), CD38 BV785 (HIT2), and CD154 PE (24–31); (4) Invitrogen: CD4 Cy5.5PE (S3.5) and Aqua LIVE/DEAD® amine viability dye; and (5) eBioscience: CXCR5-FITC (MU5UBEE).
Paris R.M., Milagres L.G., Moysi E., Okulicz J.F., Agan B.K., Ganesan A., Petrovas C, & Koup R.A. (2017). Lower Baseline Germinal Center Activity and Preserved Th1 Immunity Are Associated With Hepatitis B Vaccine Response in Treated HIV Infection. Pathogens & Immunity, 2(1), 66-88.
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2

Comprehensive Immunophenotyping by Flow Cytometry

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Flow cytometry was performed using the following directly conjugated antibodies: (1) BD Biosciences: CD3 H7APC (SK7), CD45RA Cy7PE (L48), CD20 APC-H7 (2H7), CD45RO TRPE (UCHL), CTLA-4 APC (BNI3), IgD PE (IA6-2), IgG APC (G18-145), IgM PE-Cy5 (G20-127), IL-21 Alexa 647 (3A3-N2.1), and Ki-67 Alexa700 (150503); (2) Beckman Coulter: CD127 Cy5PE (R34.34); (3) BioLegend: PD-1 BV711 (EH12.2H7), IFNg BV421 (4S.B3), TNF-α CY7PE (MAb-11), IL-2 Alexa-700 (MQ1-17H12), CCR6 BV785 (G034E3), CD27 Alexa594 (IA4CD27), CD27 BV605 (O323), CCR7-BV605 (G043H7), CD8 BV785 (SK1), ICOS Pac Blue (C398.4A), CD19 Pac Blue (HIB19), CD38 BV785 (HIT2), and CD154 PE (24-31); (4) Invitrogen: CD4 Cy5.5PE (S3.5) and Aqua LIVE/DEAD® amine viability dye; and (5) eBioscience: CXCR5-FITC (MU5UBEE).
Paris R.M., Milagres L.G., Moysi E., Okulicz J.F., Agan B.K., Ganesan A., Petrovas C, & Koup R.A. (2017). Lower Baseline Germinal Center Activity and Preserved Th1 Immunity Are Associated With Hepatitis B Vaccine Response in Treated HIV Infection. Pathogens & Immunity, 2(1), 66-88.
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3

Multiparametric Flow Cytometry of Tonsil Cells

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The following titrated, conjugated antibodies were used for flow cytometry analysis: CD3-H7APC (SK7, BD Biosciences), CD4-BV650 (SK3, BD Biosciences), CD8-Pacific Blue (RPA-T8, BD Pharmingen), CD19-FITC (J3-119, Beckman-Coulter), CD27-BV605 (O323, Biolegend), CD45RO-Cy5PE (UCHL1, BD Pharmingen), PD-1-BV711 (EH12.2H7, Biolegend), CD57-AF594 (NK-1, Novus Bio), IgD-PE (Goat polyclonal, Southern Biotech), CD20-BV570 (2H7, Biolegend), CD38-BV786 (HIT2, Biolegend). 1-2×106 tonsil-derived cells were thawed and rested for 2h in a cell culture incubator before staining. Cells were washed with PBS, BSA (0.5%), incubated (5 min, RT) with a viability dye (Aqua-dye, Invitrogen) and surface stained with titrated amounts of antibodies for 30 min at room temperature. After washing, cells were resuspended and fixed with 1% paraformaldehyde.
Oyler B.L., Valencia-Dávila J.A., Moysi E., Molyvdas A., Ioannidou K., March K., Ambrozak D., De Leval L., Fabozzi G., Woods A.S., Koup R.A, & Petrovas C. (2023). Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches. iScience, 26(8), 107261.
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