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Anti bmp2

Manufactured by ABclonal

Anti-BMP2 is a polyclonal antibody specifically designed to detect the BMP2 (Bone Morphogenetic Protein 2) protein. It is used in various research applications that involve the analysis and identification of BMP2 expression.

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2 protocols using anti bmp2

1

Antibodies Used for Cellular Analyses

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Anti-ANLN (#AMAB90660) and anti-actin (#3700) antibodies were obtained from Cell Signaling Technology. Anti-HA tag antibody (#11867423001) was obtained from Roche. Anti-TWIST1 (#A3237), and anti-BMP2 (#A0231) antibodies were purchased from ABclonal. Horse peroxidase-conjugated anti-mouse and anti-rabbit immunoglobulin G (IgG) antibodies (AP307P, AP308P) were obtained from Millipore. Horse peroxidase-conjugated anti-rat IgG (#AS028) was obtained from ABclonal.
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2

Western Blot Analysis of Protein Expression

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Total and phosphorylated protein expression levels of target genes were analyzed by Western blotting as previously described (11 (link), 15 (link), 16 (link)). The primary antibodies used in the experiment included anti-BMP2 (catalog no. A0231; Abclonal, Wuhan, China), anti-EKR1 (catalog no. 16443-1-AP; Proteintech, Wuhan, China), anti-phosphorylated EKR1 (catalog no. AP0234; Abclonal, Wuhan, China) and anti β-tubulin (catalog no. HX1829; Huaxingbio, Beijing, China). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit (catalog no. HX2030; Huaxingbio, Beijing, China) and goat anti-mouse (catalog no. HX2032; Huaxingbio, Beijing, China) were used as secondary antibodies. The membranes were then visualized using the ECL system (Tanon, Shanghai, China).
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