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Luminex platform

Manufactured by Merck Group
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Sourced in United States, Germany

The Luminex platform is a multiplex assay technology that utilizes polystyrene microspheres for the simultaneous detection and quantification of multiple analytes in a single sample. The platform employs flow cytometry principles to identify and measure various biomolecules, including proteins, nucleic acids, and small molecules.

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Lab products found in correlation

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Spelling variants of this product

Luminex xMAP platform (21 citations) Luminex Multiplex platform (9 citations) Luminex 200 platform (6 citations) Luminex FLEXMAP 3D platform (5 citations) Luminex xMAP multianalyte profiling platform (4 citations) Luminex detection platform (2 citations) Luminex 100 platform (2 citations) Luminex® xMAP Bead Array Platform (2 citations) Magpix/Luminex platform (2 citations) Luminex xMap platform multiplex assay (2 citations)

24 protocols using luminex platform

1

Cytokine Profiling of Rodent and Human Sera

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Rodent and human sera were collected, and a panel of cytokines was measured using a Luminex platform (MilliporeSigma).
Abdullahi A., Knuth C.M., Auger C., Sivayoganathan T., Parousis A, & Jeschke M.G. (2021). Adipose browning response to burn trauma is impaired with aging. JCI Insight, 6(16), e143451.
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2

Quantifying CX3CL1 in Cell-free BALF

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First-return aliquots of cell-free BALF were analyzed for CX3CL1 levels using a Luminex platform (Millipore Sigma, Burlington, MA) by the Human Immunology Core at Perelman School of Medicine.
Venosa A., Katzen J., Tomer Y., Kopp M., Jamil S., Russo S.J., Mulugeta S, & Beers M.F. (2019). Epithelial Expression of an Interstitial Lung Disease Associated Mutation in Surfactant Protein-C Modulates Recruitment and Activation of Key Myeloid Cell Populations in Mice. Journal of immunology (Baltimore, Md. : 1950), 202(9), 2760-2771.
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3

Serum Cytokine Profiling via Multiplexed Immunoassay

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Analysis of serum cytokines/chemokines was performed using a high-sensitivity immunology multiplex assay kit based on the Luminex platform (Millipore Sigma) per the manufacturer’s instructions.
Vinod N., Hwang D., Azam S.H., Van Swearingen A.E., Wayne E., Fussell S.C., Sokolsky-Papkov M., Pecot C.V, & Kabanov A.V. (2020). High-capacity poly(2-oxazoline) formulation of TLR 7/8 agonist extends survival in a chemo-insensitive, metastatic model of lung adenocarcinoma. Science Advances, 6(25), eaba5542.
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4

CCL2 Quantification in Cell-free BALF

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First-return aliquots of cell-free BALF were analyzed for CCL2 levels using a Luminex platform (Millipore Sigma, Burlington, MA) by the Human Immunology Core at Perelman School of Medicine.
Venosa A., Cowman S., Katzen J., Tomer Y., Armstrong B.S., Mulugeta S, & Beers M.F. (2021). Role of CCR2+ Myeloid Cells in Inflammation Responses Driven by Expression of a Surfactant Protein-C Mutant in the Alveolar Epithelium. Frontiers in Immunology, 12, 665818.
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5

Murine Lung Cytokine Profiling

Cited 1 time
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BAL on experimental mice was performed as previously described (52 (link)). Pulmonary cytokine measurements were performed on murine lung homogenate using a Luminex platform (MilliporeSigma) as previously described (13 (link)). IgM was measured using Bethyl Laboratories catalog E99-101 mouse IgM ELISA kit per manufacturer’s instructions. BAL fluid was diluted to 1:1,000.
Gurczynski S.J., Lipinski J.H., Strauss J., Alam S., Huffnagle G.B., Ranjan P., Kennedy L.H., Moore B.B, & O’Dwyer D.N. (2023). Horizontal transmission of gut microbiota attenuates mortality in lung fibrosis. JCI Insight, 9(1), e164572.
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6

Multiplex Cytokine Quantification

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For cytokine quantification, the Luminex® platform (Millipore, USA) was used following manufacturer’s instructions. Milliplex® Map kits (Rat Cytokine/Chemokine Panel) were used to determine the plasma concentration of interleukin (IL)-4, IL-6, IL-8, IL-10, and tumor necrosis factor (TNF)-α. Briefly, each cytokine binds to its specific antibody-coated microsphere, which contains 2 fluorochromes. This combination of fluorochromes allows for the determination of which cytokine is bound to each microsphere. Additionally, another antibody binds to the cytokine-microsphere complex, thus determining the concentration of each cytokine.
Hirotsu C., Pedroni M.N., Berro L.F., Tufik S, & Andersen M.L. (2018). Nicotine and sleep deprivation: impact on pain sensitivity and immune modulation in rats. Scientific Reports, 8, 13837.
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7

Cytokine Profiling in Diabetes

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After an overnight fasting for at least 10 hours when DK/DKA resolved, fasting blood samples were collected and centrifuged, and sera were collected for cytokine determination. The following cytokines were measured: interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-17A/CTLA-8, IL-17F, IL-21, IL-22, IL-23, interferon-γ (IFN-γ), programmed death-1 (PD-1), PD ligand 1 (PD-L1), cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4/CD152), CD40, transforming growth factor β1 (TGFβ1), TGFβ2, and TGFβ3. All these measurements of cytokines were performed using Milliplex MAP technology in the Luminex platform (Millipore, Billerica, MA). Immunology multiplex assay kits (HTH17MAG-14K-12, TGFBMAG-64K-03, and HCKPMAG-11K-04) were obtained from MERCK (MERCK Millipore Co., Billerica, MA, USA). All the collected serum samples were mixed and diluted at a ratio of 1:2 with buffer, and all samples, standards, and quality controls were assayed in accordance with the manufacturer’s instructions. Median fluorescent intensity (MFI) data were acquired using MILLIPLEX Analyst.V5.1 software, and a 5-parameter logistic method was used to calculate cytokine concentrations.
Ying L., Zhang Y., Yin J., Wang Y., Lu W., Zhu W., Bao Y, & Zhou J. (2021). Classic Type 1 Diabetes Mellitus and Fulminant Type 1 Diabetes Mellitus: Similarity and Discrepancy of Immunological Characteristics and Cytokine Profile. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 14, 4661-4670.
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8

Biomarker Profiling in Vascular Inflammation

Cited 3 times
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Biomarkers associated with macrophage activation, vascular inflammation as well as lymphocyte activation were measured. Ten blood-based biomarkers associated with macrophage activation (sCD163, sCD14, TNFα, GM-CSF, CCL2 (monocyte chemoattractant protein-1: MCP-1)), IL-1β, IL-6, IL-8, LPS, and MMP-2) [13 (link), 17 (link), 37 (link)-40 (link)], four with vascular inflammation (MPO, sICAM-1, sVCAM-1, and CRP) [22 (link), 41 (link), 42 (link)], and four with lymphocyte activation (sCD27, IFNγ, sIL-2Rα (sCD25), and IL-10) [39 (link), 43 (link), 44 (link)] were measured as previously described [27 (link)]. ELISA assays were used to measure sCD14, sCD163, matrix metalloprotease-2 (MMP-2), (R&D Systems, Minneapolis, MN) and sCD27 (eBioscience, San Diego, CA). Plasma LPS was measured using the limulus amebocyte assay (LAL) Chromogenic Endpoint Assay. The remaining biomarkers were measured using the magnetic bead-based Luminex platform (Millipore, Darmstadt, Germany). Supplemental figure 1 lists lower limits of detection, inter-assay coefficients of variance, and the distributions of raw biomarker vaues for each assay.
Kim-Chang J.J., Donovan K., Loop M.S., Hong S., Fischer B., Venturi G., Garvie P.A., Kohn J., Rendina H.J., Woods S.P., Goodenow M.M., Nichols S.L, & Sleasman J.W. (2019). Higher soluble CD14 levels are associated with lower visuospatial memory performance in Youth with HIV (YWH). AIDS (London, England), 33(15), 2363-2374.
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9

Cytokine Profile Post-Immunization

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Serum was collected 24 h after immunization at stored at -70°C until further analysis. Cytokine protein levels were determined by cytokine-specific beads using the Luminex platform (Millipore).
Bosteels C., Fierens K., De Prijck S., Van Moorleghem J., Vanheerswynghels M., De Wolf C., Chalon A., Collignon C., Hammad H., Didierlaurent A.M, & Lambrecht B.N. (2021). CCR2- and Flt3-Dependent Inflammatory Conventional Type 2 Dendritic Cells Are Necessary for the Induction of Adaptive Immunity by the Human Vaccine Adjuvant System AS01. Frontiers in Immunology, 11, 606805.
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10

Circadian Regulation of Stress Hormones

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Mice were killed by cervical dislocation and trunk blood was collected. For the gene expression studies, mice were killed for brain dissections at 2 distinct points of the day/light cycle (+ 3 h lights on ~ 10am and + 3 h lights off ~ 10 pm). Brains were immediately removed from the skull and the hypothalamus was fresh dissected on ice, snap frozen in liquid nitrogen then transferred to storage at − 80 °C until further use. Whole blood was left to clot for 30 min before separation of serum by centrifugation at 1100 g for 15 min, which was stored at − 20 °C until subsequent analysis. Corticosterone concentrations were assayed using an EIA as per manufacturer’s instructions (#501320, Caymen Chemical, Ann Arbor, MI, USA). ACTH concentrations were determined by a commercial service provider (Cardinal Bio-Research, New Farm, QLD, Australia) using a Millipore Luminex platform with the MILIPLEX MAP mouse bone panel (MBNMAG-41K). One control sample from the ‘lights on + 3 h’ group failed and was excluded from the analysis.
Fennell K.A., Busby R.G., Li S., Bodden C., Stanger S.J., Nixon B., Short A.K., Hannan A.J, & Pang T.Y. (2020). Limitations to intergenerational inheritance: subchronic paternal stress preconception does not influence offspring anxiety. Scientific Reports, 10, 16050.
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