Rsa 3 microstrain analyzer

Manufactured by TA Instruments

Sourced in United States

The RSA III microstrain analyzer is a laboratory instrument designed to measure and analyze microstrain levels in materials. It provides precise measurements of strain, stress, and other mechanical properties. The core function of the RSA III is to quantify the deformation characteristics of various materials under controlled conditions.

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Lab products found in correlation

Ar g2, by TA Instruments (1 mentions) Ta orchestrator software, by TA Instruments (1 mentions)

7 protocols using rsa 3 microstrain analyzer

Hydrogel Mechanical Characterization

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One millimeter thick hydrogels were
prepared at 5%, 7.5%, or 10% (w/v) then soaked overnight in PBS at
37 °C and briefly rinsed with PBS to remove any unpolymerized
material before mechanical testing. Compression testing was performed
on a RSA III microstrain analyzer (TA Instruments, New Castle, DE).
Samples were compressed at a constant rate of 0.003 mm/s. The slope
of the initial linear range was taken to be the compressive modulus
(N = 4). For rheology, 1 mm thick hydrogels were
cast between two Sigmacote-treated glass slides. Gels were then cut
out into 10 mm diameter disks with a biopsy punch and soaked in PBS
at 37 °C overnight prior to testing. Rheological measurements
were performed using coarse stainless steel platens to eliminate slippage
on an AR-G2 (TA Instruments) with 10% axial strain. Samples were swept
to establish the linear regions where G′ and G″ were reported.

Schweller R.M, & West J.L. (2015). Encoding Hydrogel Mechanics via Network Cross-Linking Structure. ACS Biomaterials Science & Engineering, 1(5), 335-344.

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Compression Testing of PEG-PQ Hydrogels

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Hydrogels (1 mm thick) were prepared from PEG-PQ or PEG-PQ(alloc) with a final polymer density of 5% (w/v) and allowed to swell overnight in PBS at 37°C. Hydrogels were briefly rinsed with PBS to remove any unpolymerized material before mechanical testing. Compression testing was performed on a RSA III microstrain analyzer (TA Instruments, New Castle, DE) using a 15 mm plate. Samples were compressed at a constant rate of 0.003 mm s⁻¹. Since, PEG-based hydrogels are isotropic, testing was only performed in one direction.^{37 (link)}

Schweller R.M., Wu Z.J., Klitzman B, & West J.L. (2017). Reducing Stiffness of Protease Sensitive and Cell Adhesive PEG Hydrogels Promotes Neovascularization In Vivo. Annals of biomedical engineering, 45(6), 1387-1398.

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Compressive Modulus of Vinyl-Acrylate Hydrogels

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Gels made 1 mm in height were swollen overnight in phosphate buffered saline (1× PBS, pH 7) at 37 °C, then rinsed with PBS. They were placed on the parallel plates of an RSA III microstrain analyzer (TA Instruments, New Castle, DE, USA) and compressed at a strain rate of 0.003 mm/sec. From the resulting stress–strain curve, the slope of the linear region immediately following the toe region (65–70% strain) was taken as the compressive modulus (Figure S1). For each soluble vinyl monomer, gels were tested with vinyl to acrylate molar ratios of 0:1, 1:1, 2:1, 3:1, and 4:1. Four hydrogels were tested per group.

Chapla R., Alhaj Abed M, & West J. (2020). Modulating Functionalized Poly(ethylene glycol) Diacrylate Hydrogel Mechanical Properties through Competitive Crosslinking Mechanics for Soft Tissue Applications. Polymers, 12(12), 3000.

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Tensile Properties of Mouse Lenses with GsMTx4

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Changes in the tensile properties of the control mouse lenses (3- to 4-weeks-old) and lenses incubated with GsMTx4 (2.44 μM for 48 h) were analyzed using an RSA III micro-strain analyzer (TA Instruments, New Castle, DE, USA) equipped with parallel plate tools, as we described previously [36 (link)]. Briefly, lens compression was carried out between 2 8-mm plates, which were attached to the parallel plate tools and mounted on actuator shafts. All measurements were performed at room temperature while the lens was submerged in the culture medium. The samples were strained at a constant rate of 0.05 mm/s for a total of 35 s until sample rupture occurred. Data were acquired and plotted in real time using TA Orchestrator software. Applied stress was calculated by dividing the measured changes in the applied force by the area. The slope values before lens rupture were calculated from the linear range of the slope and plotted in Microsoft excel. The percent change in Young’s modulus between the control and GsMTx4-incubated lenses is shown as a histogram output.

Allen A., Maddala R., Eldawy C, & Rao P.V. (2022). Mechanical Load and Piezo1 Channel Regulated Myosin II Activity in Mouse Lenses. International Journal of Molecular Sciences, 23(9), 4710.

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Assessing Lens Compressive Stiffness

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Changes in the compressive stiffness of WT and mdx^3cv mouse lenses were analyzed using an RSA III micro-strain analyzer (TA Instruments, New Castle, DE) equipped with parallel plate tools as we described earlier [6 (link)]. The samples were strained at a constant rate of 0.05 mm/s for 35 s until sample rupture occurred. Data were acquired and plotted in real-time using TA Orchestrator software. Applied stress was calculated by dividing measured changes in applied force by the area. Slope values before lens rupture were calculated from the linear range of slope[6 (link)].

Karnam S., Skiba N.P, & Rao P.V. (2020). Biochemical and biomechanical characteristics of dystrophin-deficient mdx3cv mouse lens. Biochimica et biophysica acta. Molecular basis of disease, 1867(1), 165998.

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Mechanical Properties of Compressed PSIs

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To evaluate the mechanical properties of PSIs prepared with varying compression forces, a three-point bending test was performed on a RSAIII micro-strain analyzer (TA Instruments, USA) fitted with three-point bending geometries. For each measurement, a 10-mm diameter PSI tablet was placed on the lower 3-point bending tool, and the sample was then tested with a 35 N load cell at a strain rate of 0.05 mm/s until a fracture was observed within the tablet.

Maturavongsadit P., Paravyan G., Kovarova M., Garcia J.V, & Benhabbour S.R. (2020). A new engineering process of biodegradable polymeric solid implants for ultra-long-acting drug delivery. International Journal of Pharmaceutics: X, 3, 100068.

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Quantifying Granular Packing Mechanics via Flat Indentation

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Flat-punch indentation experiments were conducted with a TA instruments RSA III microstrain analyzer and indented with a 8-mm-cylindrical flat punch at two displacement rates, 0.05 and 4 mm/s. Packings of cornstarch in water and ethanol were made by adding solvent into granular cornstarch. In the case for water, we mixed the water and first allowed it to be absorbed. Experiments were conducted after the mixture settled into granular packing.
For flat-punch contact mechanics calculations, we used a standard rigid cylindrical indenter into flat plane scenario:

F = 2 a E^{*} d,

where F is the normal force, a is the cylinder radius (4 mm), d is the indentation depth, and E ~ E*3/4, assuming ν ~ 0.5 and a very stiff cylinder (brass).
For sand particle–particle contact force scale estimate, we used a contact between two spheres:

F = \frac{4}{3} E^{*} R^{1 ∕ 2} d^{3 ∕ 2},

where F is the normal force, E ~ E*3/2, R is the effective radius (1/2 the particle radius), and d is the displacement.

Chen D.Z., Zheng H., Wang D, & Behringer R.P. (2019). Discontinuous rate-stiffening in a granular composite modeled after cornstarch and water. Nature Communications, 10, 1283.

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