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Edta blood collecting tubes

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EDTA blood collecting tubes are used to collect and store whole blood samples. They contain the anticoagulant EDTA (Ethylenediaminetetraacetic acid) which prevents the blood from clotting. These tubes are designed to maintain the integrity of the blood sample for downstream laboratory analysis.

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Lab products found in correlation

Trucount tube, by BD (3 mentions) Facscanto 2, by BD (2 mentions) Multitest imk kit, by BD (2 mentions) Multitest cd3fitc cd8pe cd45percp cd4apc, by BD (2 mentions) Multitest cd3 fitc cd16 cd56 pe cd45percp cd19apc, by BD (2 mentions) Facscanto plus, by BD (1 mentions) Lysing solution, by BD (1 mentions) Multitest imk kit lysing solution, by BD (1 mentions)

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EDTA tubes (290 citations)

3 protocols using edta blood collecting tubes

1

Multicolor Flow Cytometric Phenotyping of T Cell Subsets

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The percentages and AC of the Tn/Tm assay were performed by the single platform technique using the ten-color flow cytometer (BD FACS Canto plus: U6573380-00541) (37 (link)). The reagents include APC-H7 Mouse Anti-Human CD3 antibody (Catalog NO: 663490), PE-Cy7 Mouse Anti-Human CD4 antibody (Catalog NO: 663493), V500-C Mouse Anti-Human CD45 antibody (Catalog NO: 662912), APC Mouse Anti-Human CD8 antibody (Catalog NO: 663524), BV421 Mouse Anti-Human CD62L antibody (Catalog NO: 563862), PerCP-Cy 5.5 Mouse Anti-Human CD95 antibody (Catalog NO: 561655), PE Mouse Anti-Human CD45RO antibody (Catalog NO: 663530), FITC Mouse Anti-Human CD45RA antibody (Catalog NO: 662840), and BD Lysing Solution (Catalog NO: 349202). The EDTA blood collecting tubes and BD Trucount tubes (Catalog NO: 340334) were also purchased from BD Biosciences, USA. The panel of phenotypic characterization of T cells was identified according to their differentiation status (Figure 2), such as naïve T cells (Tn) (CD45RA+ CD45RO- CD62L+ CD95-), T memory stem cells (Tscm) (CD45RA+ CD45RO- CD62L+ CD95+), central memory T cells (Tcm) (CD45RA- CD45RO+ CD62L+ CD95+), effector memory T cells (Tem) (CD45RA- CD45RO+ CD62L- CD95+), and terminal effector T cells (Tte) (CD45RA+ CD45RO- CD62L- CD95+) (37 (link)).
Zhang G., Liu A., Yang Y., Xia Y., Li W., Liu Y., Zhang J., Cui Q., Wang D., Liu X., Guo Y., Chen H, & Yu J. (2022). Clinical predictive value of naïve and memory T cells in advanced NSCLC. Frontiers in Immunology, 13, 996348.
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2

Flow Cytometry Analysis of Lymphocyte Subsets

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Lymphocyte subsets were assayed by using a lyse/no-wash procedure based on a single-platform technique of ten-color flow cytometry (BD FACS Canto II : U6573380-00541, USA). The reagents were BD Multitest IMK kit (catalog no.: 662965) containing BD Multitest CD3FITC/CD8PE/CD45PerCP/CD4APC and BD Multitest CD3 FITC/CD16+CD56+PE/CD45PerCP/CD19APC, and BD Multitest IMK kit lysing solution (catalog no.: 91–1087). The EDTA blood collecting tubes and trucount tubes (catalog no.: 340334) were also purchased from BD Biosciences, USA.
Liu A., Xia Y., Li W., Zhang G., Liu Y., Ye S., Zhao Z.R., Yang Y., Jia Y., Guo Y., Liu X., Chen H, & Yu J. (2022). The Predictive Value of Changes in the Absolute Counts of Peripheral Lymphocyte Subsets for Progression and Prognosis in Breast Cancer Patients. Contrast Media & Molecular Imaging, 2022, 3444360.
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3

Lymphocyte Subset Analysis by Flow Cytometry

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The detection of lymphocyte subsets were performed by using a lyse/no-wash procedure based on a single-platform technique by ten-color flow cytometry (BD FACS Canto II: U6573380-00541). The main reagents were BD Multitest IMK kit (340503) containing (BD Multitest CD3 FITC/CD8PE/CD45PerCP/CD4APC and BD Multitest CD3 FITC/CD16 + CD56PE/CD45PerCP/CD19APC), BD Multitest IMK kit lysing solution (340503); The EDTA blood collecting tubes and trucount tubes (340334) were also from BD Biosciences.
Xia Y., Li W., Li Y., Liu Y., Ye S., Liu A., Yu J., Jia Y., Liu X., Chen H, & Guo Y. (2020). The clinical value of the changes of peripheral lymphocyte subsets absolute counts in patients with non-small cell lung cancer. Translational Oncology, 13(12), 100849.
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