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Fluconazole flz

Manufactured by Pfizer
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Fluconazole (FLZ) is a laboratory product that functions as an antifungal agent. It is a synthetic triazole compound used for the treatment of various fungal infections.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Sabouraud dextrose agar, by Merck Group (2 mentions) Potato dextrose broth (pdb), by Merck Group (1 mentions) Yeast peptone dextrose broth, by Thermo Fisher Scientific (1 mentions) Crystal violet, by Merck Group (1 mentions) Ethidium bromide, by Merck Group (1 mentions) Acridine orange, by Merck Group (1 mentions) 2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions) Sabouraud dextrose broth (sdb), by Merck Group (1 mentions) 3 n morpholino propanesulfonic acid mops, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Glacial acetic acid, by Carlo Erba (1 mentions) Thymoquinone, by Merck Group (1 mentions)

3 protocols using fluconazole flz

1

Antifungal Screening of Candida Species

Cited 1 time
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Three American Type Culture Collection (ATCC) yeast species, including Candida albicans ATCC 36082 (fluconazole-resistant), Candida glabrata ATCC 2001 (fluconazole susceptible), Candida parapsilosis ATCC 22019 (fluconazole-resistant) were obtained from Oxoid, UK and Candida albicans CL1 (fluconazole-resistant) was recovered from scalp infection in this study [62 (link),63 (link),64 (link)]. Pure cultures were grown onto sabouraud dextrose agar (SDA, Merck, Germany) at 28 °C for 48 h, as described by Pfaller et al. [65 (link)]. Standard solutions of 1500 µg/mL of fluconazole (FLZ) (Pfizer, Manhattan, NY, USA) and synthetic compounds were prepared in DMSO (Sigma Aldrich, Germany).
Khan F.A., Yaqoob S., Ali S., Tanveer N., Wang Y., Ashraf S., Hasan K.A., Khalifa S.A., Shou Q., Ul-Haq Z., Jiang Z.H, & El-Seedi H.R. (2022). Designing Functionally Substituted Pyridine-Carbohydrazides for Potent Antibacterial and Devouring Antifungal Effect on Multidrug Resistant (MDR) Strains. Molecules, 28(1), 212.
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2

Antifungal Susceptibility Assay Protocol

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RPMI 1640, phosphate buffered saline (PBS), and yeast peptone dextrose broth (YPD) were obtained from (Thermo Fisher Scientific, Waltham, MA, USA); 3-N-morpholinopropanesulfonic acid (MOPS) was obtained from (Merck); propidium iodide (PI), 2′,7′-dichlorofluorescin diacetate (DCFH-DA), potato dextrose broth (PDB), sabouraud dextrose agar (SDA), sabouraud dextrose broth (SDB), amphotericin B (AFB), acridine orange (OA), ethidium bromide (EB), and crystal violet used in this study were obtained from Sigma-Aldrich, United States; glacial acetic acid was obtained from Carlo Erba Reagents, Italy; and Fluconazole (FLZ) was obtained from Pfizer.
Contreras Martínez O.I., Angulo Ortíz A, & Santafé Patiño G. (2022). Mechanism of Antifungal Action of Monoterpene Isoespintanol against Clinical Isolates of Candida tropicalis. Molecules, 27(18), 5808.
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3

Antifungal Activity of Thymoquinone and Azoles

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Aliquots of 400 uL/mL were prepared in dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA) and kept in storage at −20 °C. Thymoquinone (Sigma-Aldrich, USA) was used at concentrations of 400–0.78 µg/mL [35 (link)], Amphotericin B (AMB) (Sigma Chemical Corporation, St. Louis, MO, USA) was used at concentrations of 0.016–16 μg/mL, Fluconazole (FLZ) (Pfizer, New York, NY, USA) was used at concentrations of 0.063–64 μg/mL, and Itraconazole (ITZ) (Santa Cruz Biotech, Dallas, TX, USA) was used at concentrations of 0.016–16 μg/mL.
Nouri N., Mohammadi S.R., Beardsley J., Aslani P., Ghaffarifar F., Roudbary M, & Rodrigues C.F. (2023). Thymoquinone Antifungal Activity against Candida glabrata Oral Isolates from Patients in Intensive Care Units—An In Vitro Study. Metabolites, 13(4), 580.
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