L glutathione reduced

Hoechst 33258, and calcium ionophore III were obtained from Sigma (St Louis, MO, USA). Acridine orange (AO)/ethidium bromide (EB) double stain assay kit, L-glutathione (Reduced) and tunicamycin were purchased from Solarbio Science & Technology Co. (Beijing, China). Protein A/G-agarose was from Biogot Technology Co. (Nanjing, Jiangsu, China). Lipofectamine RNAiMAX and Lipofectamine 2000 were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Pronase was from Roche Diagnostics GmbH (Mannheim, Germany) and recombinant histone H3 was purchased from Active Motif (CA, USA). LysoTracker, MitoSox, MitoTracker Green, and MitoTracker Red were purchased from Thermo Fisher Scientific (Waltham, Ma, USA). ER-Red and DIOC6 (3) were acquired from Nanjing KeyGen Biotech Co. (Nanjing, Jiangsu, China). Fluo 3-AM special packaging was purchased from Dojindo Laboratorie (Kumamoto-ken, Kyushu, Japan). Thapsigargin was obtained from ACROS Organics (Belgium). LysoSensor^TM Yellow/Blue DND-160 (PDMPO) was purchased from Yeasen (Shanghai, China). DO₂, NaOD, and DCl were acquired from J&K Scientific (Beijing, China). R18 peptide was obtained from Enzo Life Sciences (New York, USA).

Fresh grass carp was purchased from Rainbow mall (116°02′E, 28°67′N) in Nanchang, Jiangxi province, China. Alcalase and protamex were provided by Novozymes (Bagsvaerd, Denmark) and Ruiyang Biotechnology Co. Ltd (Jiangsu, China), respectively. Formic acid, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS), and trifluoroacetic acid (TFA) were purchased from Sigma‐Aldrich (Sigma, St. Louis, MO). Glutathiose (GSH), ferrozine, l‐glutathione reduced, pyrogallol, and other reagents were obtained from Solarbio (Beijing, China). Sephadex G‐25 was purchased from GE Healthcare (Pittsburgh, USA). Formic acid and TFA were of chromatographic grade, while other reagents were of analytic grade.