N2a cells that transfected with pCAGGS or pCA-Trim72-flag for 48 h were fixed with 4% (v/v) paraformaldehyde for 15 min at room temperature then washed three times with PBS. The cells were permeabilized in PBS containing 0.3% Triton X-100 for 5 min at 4°C, then blocked in 5% BSA buffer for 2 h at 37°C. Cells were then incubated with anti-flag antibody (MBL, M185-3) for 1.5 h at 37°C. After washed three times with PBS, cells were then incubated with Alexa Fluor 488 goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody (Invitrogen, 11001) for 45 min at 37°C. The cells were then stained with DAPI for 10 min and again washed three times with PBS, and then imaged with a NiKon microscope (Ti2-A).
Alexa fluor 488 goat anti mouse igg h l cross adsorbed secondary antibody
Manufactured by Thermo Fisher Scientific
Alexa Fluor 488 goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody is a fluorescently labeled secondary antibody that binds to mouse immunoglobulins. It is designed for use in immunofluorescence and other antibody-based detection techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alexa fluor 488 goat anti mouse igg h l cross adsorbed secondary antibody
1
Immunofluorescence Analysis of Trim72 in N2a Cells
2
Antibody Panel for Neuronal Markers
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The antibody against Armcx1 (PA5-50911) was purchased from Invitrogen; the Armcx1 antibody (SAB2100153) was from Sigma Aldrich; the antibodies against cleaved caspase-9 (9507), β-actin (4970), and β3-tubulin (4466) were from Cell Signaling Technology; and the anti-NeuN antibody -a neuronal marker (ab104224), NeuN antibody (ab177487), GFAP antibody (ab134436), and Iba1 antibody (ab48004) were from Abcam. The secondary antibodies for western blotting, including goat anti-rabbit IgG-HRP (bs13278) and goat anti-mouse IgG-HRP (bs12478), were purchased from Bioworld. The secondary antibodies for immuno uorescence, including Alexa Fluor 488 donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody (A21206), Alexa Fluor 555 goat anti-rabbit IgG (H+L) crossadsorbed secondary antibody (A21428), Alexa Fluor 488 goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody (A11001), Alexa Fluor 555 donkey anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody (A31570), Alexa Fluor 555 goat anti-chicken IgY (H+L) secondary antibody (21437), Alexa Fluor Plus 555 donkey anti-goat IgG (H+L) highly cross-adsorbed secondary antibody (A32816), and Alexa Fluor 633 goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (A-21070) were from Invitrogen.
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